Egr1-EGFP transgenic mouse allows in vivo recording of Egr1 expression and neural activity

Wang, Guangyu; Xie, Hong; Hu, Yi; Chen, Qinan; Liu, Chenhui; Liu, Kaiyuan; Yan, Yuze; Guan, Ji‐Song

doi:10.1016/j.jneumeth.2021.109350

Cited by 7 publications

(11 citation statements)

References 38 publications

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“…However, these potential differences in decay kinetics and expression levels do not completely mask the correlation between IEG expression levels and reporter proteins. In post-mortem histological stainings the expression of GFP in these mouse lines overlaps well with the expression levels of the IEGs (Barth et al, 2004;Okuno et al, 2012;Wang et al, 2021;Xie et al, 2014;Yassin et al, 2010). Thus, reporter protein levels reflect a filtered version of IEG expression levels, and the two are likely related by a monotonic function.…”

Section: Discussionmentioning

confidence: 73%

“…It is well established that both neuronal activity and plasticity are linked to the expression of immediate early genes (Dudek, 2008; Mahringer et al, 2019; Minatohara et al, 2015; Wang et al, 2021; Yap and Greenberg, 2018). Comparably little, however, is known about how specific functional characteristics of neurons relate to the expression of immediate early genes.…”

Section: Discussionmentioning

confidence: 99%

“…Ever since the discovery that the expression of the transcription factor c-Fos can be induced by electrical or chemical stimulation in neurons (Greenberg and Ziff, 1984), the expression of IEGs has been used as a marker for neuronal activity (Bullitt, 1990;Guzowski et al, 1999;Jarvis et al, 2000;Knapska and Kaczmarek, 2004;Minatohara et al, 2015;Morgan et al, 1987;Ramírez-Amaya et al, 2005;Reijmers et al, 2007;Wang et al, 2021). Based on the discovery that certain forms of episodic memory can be reactivated by artificially activating an ensemble of neurons characterized by high IEG expression levels during memory acquisition (Denny et al, 2014;Garner et al, 2012;Liu et al, 2012;Ramirez et al, 2013), it has been speculated that IEG expression is related not simply to neuronal activity per se, but to the induction of activity-dependent plasticity (Holtmaat and Caroni, 2016;Josselyn et al, 2015;Kaplan et al, 1996).…”

Section: Introductionmentioning

confidence: 99%

“…IEG products play a critical role in synaptic and neuronal plasticity during learning (Chowdhury et al, 2006; Fleischmann et al, 2003; Gandolfi et al, 2017; Jones et al, 2001; Messaoudi et al, 2007; Rial Verde et al, 2006; Shepherd and Bear, 2011; Shepherd et al, 2006; Tzingounis and Nicoll, 2006; Vazdarjanova et al, 2006; Veyrac et al, 2014; Waung et al, 2008) and are necessary for long-term memory consolidation (Bozon et al, 2003; Fleischmann et al, 2003; Guzowski, 2002; Guzowski and McGaugh, 1997; Guzowski et al, 2000; Jones et al, 2001; Ploski et al, 2008; Yasoshima et al, 2006). Ever since the discovery that the expression of the transcription factor c-Fos can be induced by electrical or chemical stimulation in neurons (Greenberg and Ziff, 1984), the expression of IEGs has been used as a marker for neuronal activity (Bullitt, 1990; Guzowski et al, 1999; Jarvis et al, 2000; Knapska and Kaczmarek, 2004; Minatohara et al, 2015; Morgan et al, 1987; Ramírez-Amaya et al, 2005; Reijmers et al, 2007; Wang et al, 2021). Based on the discovery that certain forms of episodic memory can be reactivated by artificially activating an ensemble of neurons characterized by high IEG expression levels during memory acquisition (Denny et al, 2014; Garner et al, 2012; Liu et al, 2012; Ramirez et al, 2013), it has been speculated that IEG expression is related not simply to neuronal activity per se , but to the induction of activity-dependent plasticity (Holtmaat and Caroni, 2016; Josselyn et al, 2015; Kaplan et al, 1996).…”

Section: Introductionmentioning

confidence: 99%

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Functional correlates of immediate early gene expression in mouse visual cortex

Mahringer

Zmarz

Okuno

et al. 2020

Preprint

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The expression of immediate early genes (IEGs) in visual cortex can be triggered by visual input and is associated with certain forms of neuronal plasticity. How IEG expression in cortical neurons relates to neuronal activity or experience-dependent changes of neuronal activity, however, is still unclear. Using three transgenic mouse lines that express GFP under the control of different IEG promoters (c-fos, egr1 or Arc), we recorded both neuronal activity and IEG expression levels in primary visual cortex before and after a mouse’s first visual exposure, and subsequent visuomotor learning. We found that expression levels of all three IEGs correlated positively with neuronal activity, and that different IEGs are preferentially expressed in different functional cell types. Neurons with strong motor-related activity preferentially expressed EGR1 while neurons that developed strong visually driven activity preferentially expressed Arc. Our findings suggest that during functional development of visual cortex different IEGs are preferentially expressed in neurons that receive different functional types of input.

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Section: Discussionmentioning

confidence: 73%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Functional correlates of immediate early gene expression in mouse visual cortex

Mahringer

Zmarz

Okuno

et al. 2020

Preprint

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“…As newborn neurons are actively involved in memory formation and extinction, we labelled newborn neurons with the retrovirus, which specifically integrates into proliferating cells to express mCherry in newborn neurons, but not other cells. To ask if the newborn neurons are engaged by the learning task, we employed EGR1‐EGFP transgenic mice (Wang et al, 2021; Xie et al, 2014) to label the memory‐activated granule cells in dentate gyrus (DG). As the retrovirus (RV) labels neurons right at birth, we utilized the RV to label the newborn neurons in DG.…”

Section: Resultsmentioning

confidence: 99%

Suv39h1 regulates memory stability by inhibiting the expression of Shank1 in hippocampal newborn neurons

Chen

Ding

Guo

et al. 2022

Eur J of Neuroscience

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Adult newborn neurons are involved in memory encoding and extinction, but the neural mechanism is unclear. We found the adult newborn neurons at 4 weeks are recruited by learning and subjected to epigenetic regulations, consequently reducing their ability to be re‐recruited later. After removal of the epigenetic blockage, Suv39h1 KO mice showed an increased recruiting number of aged newborn neurons and enhanced flexibility in learning tasks. Besides NRXN1, we found SHANK1, the synaptic scaffold protein, is one of the major targets of Suv39h1, regulating memory stability. Expression of Shank1 is transiently engaged to enhance synaptogenesis during learning and is strongly suppressed by Suv39h1 from 5 h after learning. Exogenously overexpression of Shank1 in dentate gyrus increased the density of mushroom spines and decreased the persistency of old memories. Our study indicated the activity‐regulated epigenetic modification in newly matured newborn neurons in hippocampus insulates temporally distinct experiences and stabilizes old memories.

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