Egress of budded virions of Autographa californica nucleopolyhedrovirus does not require activity of Spodoptera frugiperda HSP/HSC70 chaperones

Lyupina, Yu. V.; Orlova, Olga V.; Abaturova, Svetlana B.; Beljelarskaya, Svetlana N.; Lavrov, Andrey; Mikhailov, Victor S.

doi:10.1016/j.virusres.2014.08.002

Cited by 11 publications

(14 citation statements)

References 40 publications

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“…Others used VER155008 to investigate stress granule formation (Bounedjah et al, 2014), the pathogenicity and virulence of Entamoeba histolytica (Santos et al, 2015) to confirm a role for HSP70 in the infection cycle of baculoviruses. When VER-155008 was added early in infection, the synthesis of viral proteins, genome replication and the production of budded virions were markedly inhibited by the chemical tool indicating the dependence of virus reproduction on HSP70 (Lyupina et al, 2014). Other studies used VER155008 to provide a link between HSP70 and the NEK protein kinase family in mitotic progression (O’regan et al, 2015).…”

Section: Hsp70 Probesmentioning

confidence: 99%

Chemical Tools to Investigate Mechanisms Associated with HSP90 and HSP70 in Disease

Shrestha

Patel

Chiosis

2016

Cell Chemical Biology

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The chaperome is a large and diverse protein machinery composed of chaperone proteins and a variety of helpers, such as the co-chaperones, folding enzymes and scaffolding and adapter proteins. Heat shock protein 90s and 70s (HSP90s and HSP70s), the most abundant chaperome members in human cells, are also the most complex. As we have learned to appreciate, their functions are context dependent and manifested through a variety of conformations that each recruit a subset of co-chaperone, scaffolding and folding proteins and which are further diversified by the post-translational modifications each carry, making their study through classic genetic and biochemical techniques quite a challenge. Chemical biology tools and techniques have been developed over the years to help decipher the complexities of the HSPs and this review will provide an overview of such efforts with focus on HSP90 and HSP70.

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Section: Hsp70 Probesmentioning

confidence: 99%

Chemical Tools to Investigate Mechanisms Associated with HSP90 and HSP70 in Disease

Shrestha

Patel

Chiosis

2016

Cell Chemical Biology

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“…6c, Supplementary Table 6), that is thought to regulate host Hsp70 [108,109] among the top 5% viral genes expressed in our study. S. frugiperda Hsp70 has been reported to be a required gene to express AcMNPV genes and complete the infection cycle [110]. Even though the infected hemolymph transcriptomes in our study contain the transcripts potentially coding for Hsp70 (TR12464|c0_g1_i1, DN38479_c0_g1_i1, Supplementary Table 3), it was not significantly induced during the time of sampling (Supplementary Table 4).…”

Section: Viral-host Co-transcriptional Interactionsmentioning

confidence: 65%

A tale of two transcriptomic responses in agricultural pests via host defenses and viral replication

Pantha

Subbaiah

et al. 2020

Preprint

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BackgroundAutographa californica Multiple Nucleopolyhedrovirus (AcMNPV) is a baculovirus with a high potential for its use as a biopesticide against arthropod pests. The budded form of the virus causes a systemic infection when it escapes the midgut to enter the hemolymph of susceptible hosts. Yet, the specific molecular processes underlying the biocidal activity of AcMNPV on its insect hosts are largely unknown.ResultsIn this study, we describe the transcriptional responses in two major pests, Spodoptera frugiperda and Trichoplusia ni, to determine the host-pathogen responses during AcMNPV infection, concurrently with the viral response to the host. We assembled species-specific de novo reference transcriptomes of the hemolymph to identify key transcripts that respond during pathogenesis in these arthropod models where genomic resources are sparse. We found that the suppression of transcriptional processes related to chitin, a metabolite critical for basement membrane stability and tracheal development are central in establishing a systemic infection. Synergistic transcriptional support was observed to suggest suppression of immune responses and induction of oxidative stress indicating disease progression in the host. The entire AcMNPV core genome was expressed in the host hemolymph and viral genes predominantly associated with the budded virus replication, structure, and movement were more abundant than those associated with the occlusion-derived virus. Genes known to directly arrest host cell cycle and development were among the most abundant AcMNPV transcripts in infected hosts. Interestingly, several of the host genes (e.g. Chitin synthase) that were targeted by the pathogen as revealed by our study are also targets of several chemical insecticides currently used commercially to control arthropod pests.ConclusionsOur results reveal an extensive overlap between biological processes represented by genes differently expressed in both hosts, as well as convergence on highly abundant viral genes expressed in the two hosts, providing an overview of the host-pathogen transcriptomic landscape during systemic infection. Given the diversity of AcMNPV strains that infect a wide range of insect hosts, our study provides a framework where pathogen strains could be selected to target specific host genes that facilitates modulation of the infection strength and specificity of the susceptible hosts.

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“…Imaginal discs growth factor has a signal peptide that directs it to secretory pathways, as reported in Drosophila melanogaster Meigen (Diptera: Drosophilidae) larvae (Kawamura et al, 1999;Pesch et al, 2016). other physical stresses (Li et al, 2012;Liu et al, 2017;Lyupina et al, 2014;Singh et al, 2015;Q. Zhang & Denlinger, 2010).…”

Section: Discussionmentioning

confidence: 92%

“…Considering the function of HSP 70 as chaperones that protect against stressors (J. Gu et al, 2012;Li et al, 2012;Liu et al, 2017;Lyupina et al, 2014;Shu et al, 2011;Singh et al, 2015;Q. Zhang & Denlinger, 2010), their presence in D. saccharalis suggests a similar function in response to temperature variations and others environmental stressors.…”

Section: Discussionmentioning

confidence: 99%

Protein and volatile contents in the mandibular gland of the sugarcane borerDiatraea saccharalis(Lepidoptera: Crambidae)

Borges

Teixeira

Martínez

et al. 2022

Arch Insect Biochem Physiol

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The sugarcane borer Diatraea saccharalis (Lepidoptera: Crambidae) is an important sugarcane pest and mechanical injuries caused through the mandibles can allow pathogen infections. The mandibles of D. saccharalis, as well as other insects, are associated with mandibular glands with a possible function in food intake and mouthparts lubrication; however, the chemical composition of the secretion is poorly known and its elucidation is important for the comprehensive understanding of plant–insect interactions. This study characterized some proteins and volatiles in the mandibular glands of D. saccharalis larvae. MALDI‐TOF/TOF mass spectrometry allowed the identification of 24 predicted proteins within 10 functional classes, including the transport and metabolism of carbohydrates, lipids, amino acids, and nucleotides; Posttranslational protein modifications; energy conversion; intracellular trafficking; transcription; translation; and cytoskeleton function. Metabolites identified from GC/MS analysis revealed the presence of hydrocarbons classified as alcohols, ether, alkanes, and esters with differences in their relative abundance. Linolenic acid, the most abundant metabolite found in this gland, when conjugated with amino acids, can be an elicitor in the plant‐herbivore interaction. The results suggest the occurrence of digestive and defensive biochemical components, which may contribute to understanding of the multifunctional roles of the mandibular gland secretion of D. saccharalis larvae during feeding activity.

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Egress of budded virions of Autographa californica nucleopolyhedrovirus does not require activity of Spodoptera frugiperda HSP/HSC70 chaperones

Cited by 11 publications

References 40 publications

Chemical Tools to Investigate Mechanisms Associated with HSP90 and HSP70 in Disease

Chemical Tools to Investigate Mechanisms Associated with HSP90 and HSP70 in Disease

A tale of two transcriptomic responses in agricultural pests via host defenses and viral replication

Protein and volatile contents in the mandibular gland of the sugarcane borerDiatraea saccharalis(Lepidoptera: Crambidae)

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