EgSeverin and Eg14-3-3zeta from Echinococcus granulosus are potential antigens for serological diagnosis of echinococcosis in dogs and sheep

Zhang, Xiaoli; Wei, Chenxi; Lv, Yajie; Mi, Rongsheng; Guo, Baoqiang; Rahman, Sajid Ur; Zhang, Yehua; Cheng, Long; Jia, Hao; Huang, Yan; Han, Xiangan; Gong, Hui; Chen, Zhaoguo

doi:10.1016/j.micpath.2023.106110

Cited by 1 publication

(1 citation statement)

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“…Cystic echinococcosis is a cosmopolitan disease with an annual incidence ranging from <1 to 200 per 100,000 in humans and a mortality rate of 2% which may be increased if sufficient care management is not provided [ 3 ]. Every year, CE causes the loss of about 1 million disability-adjusted life years (DALYs) and USD 3 billion in expenses, including treatment and livestock losses [ 4 ]. Elimination or control of CE is difficult, thus active surveillance of the disease should be considered a realistic goal by detecting the parasite in livestock particularly in sheep herds [ 5 ].…”

Section: Introductionmentioning

confidence: 99%

Sensitive detection of specific cell-free DNA in serum samples from sheep with cystic echinococcosis

Hadipour,

Darani,

Talebzadeh

et al. 2023

PLoS Negl Trop Dis

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Background Developing more sensitive methods for the diagnosis of echinococcosis is essential. In this study PCR assay for sensitive detection of specific cell-free DNA (cfDNA) of Echinococcus granulosusin sensu lato in the sera of the sheep naturally infected with echinococcosis was investigated. Methods To extract cfDNA from 35 infected sheep, the modified phenol-chloroform method was used for two different volumes (0.5 and 2 ml) of serum samples. From each extracted sample, two DNA volumes (5 and 10 μl) were amplified using both standard PCR and semi-nested PCR targeting NADH dehydrogenase subunit I. Results Standard and semi-nested PCR on 0.5 ml of serum samples detected Echinococcus DNA in 8 and 12 out of 35 sheep, respectively; however, using 2 ml of serum samples, they detected 24 and 27 samples. By increasing the volume of template DNA, the PCRs could detect 29 and 33 out of 35 samples. The results were confirmed by sequencing of randomly selected PCR amplicons and comparing them with GenBank databases. Conclusions Larger volumes of serum for DNA extraction, greater volumes of DNA template for PCR, and employing a semi-nested PCR protocol, increased the sensitivity of PCR to 95%. This approach can also be applied to the diagnosis of echinococcosis in humans.

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Section: Introductionmentioning

confidence: 99%