Egyptian Olea europaea leaves bioactive extract: Antibacterial and wound healing activity in normal and diabetic rats

Abstract

Background and aim In vitro activity evaluation of Egyptian Olea europaea leaves extracts, and in vivo healing activity assessment of the newly prepared ointment of Olea europaea leaves extracts mingled with Shea butter. Experimental procedure Different extraction methods and solvents were used to extract Egyptian Olea europaea bioactive agent(s). Antibacterial, scavenging activity and in-vivo evaluation of wound repair potentiality of Olea europaea extract were examined in normal and diabetic experimental rat models with induced circular excisions. Results and conclusion Olive leaves extract of Tanta was selected as the most active agent against Methicillin-resistant S. aureus (MRSA), with MIC value 15.6 μg/ml. Moreover, checkerboard dilution technique approved that the interaction between Tanta LEM crude extract and Ciprofloxacin was synergistic. Scavenging activity of the extract against DPPH free radicals was 87.55% at concentration of 50 μg/ml. In vivo normal and diabetic experimental rats treated with Shea butter: Tanta LEM extract (1:3 w/v) showed the maximum wound contraction and healing activity.

Combination of Two Kinds of Medicated Microparticles Based on Hyaluronic Acid or Chitosan for a Wound Healing Spray Patch

“…This data appears in contrast to other literature studies in which OLE was found to be active both as an antibacterial and an accelerator of wound healing. The concentrations tested were higher than that applied in the present work [37]. In another work, it was shown in vivo that wound healing was promoted by olive leaf extracts and that this effect was greater the greater was the scavenging ability of the extract [38].…”

Synergistic Effect of Polyphenol-Rich Complex of Plant and Green Propolis Extracts with Antibiotics against Respiratory Infections Causing Bacteria

“…Anti-staphylococcal activity is characteristic for all three extracts. Compared to some published studies, the MIC value of complex exceeds that of individual extracts, e.g., 2.68 mg/mL of olive leaf extract reported by Pereira et al and 2.5 mg/mL by Karygianni et al, compared to 0.78 mg/mL in our study [ 21 , 23 ]; some other authors reported higher activity, e.g., a MIC value of 15.6 μg/mL of olive extract against MRSA [ 82 ], or similar results, such as MIC values for propolis in the range of 0.39–0.78 mg/mL [ 41 ]. In general, data about propolis and olive leaf extract activity against S. aureus vary widely among studies due to different collection sites and extraction methods [ 22 , 28 , 29 , 44 , 48 , 83 ].…”

Silver/Snail Mucous PVA Nanofibers: Electrospun Synthesis and Antibacterial and Wound Healing Activities

“…PVA/AgNPs-SM nanofiber samples with standard dimensions (1 cm × 1 cm) were added to Pseudomonas aeruginosa suspension (10 2 CFU/mL). After 24 h of incubation, the nanofibers were collected and examined under an electron microscope [ 24 ].…”