EHTIC study: Evaluation of a new hemostatic agent based on tissue factor in skin grafting procedures

Rojas, Santiago; Caz, M. Dolores Pérez del; Vico, Juan Ramón Esteban; Villaverde, Eloísa; Llinas, Abel; Martínez, José Vicente; Brage, Carlos; Valero, Jordi Feo; Rodríguez, Alba González; Barreiro, Juan Garcia; López-Suso, Eugenia; Fernández-Cañamaque, J.L.; López, Ramón; Murat, J

doi:10.1016/j.burns.2017.01.007

Cited by 6 publications

(3 citation statements)

References 25 publications

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“…For example, sodium citrate may cause aggregation of platelets, resulting in inaccurate platelet counts that impact the clinical outcome [ 12 , 18 , 19 ]. Meanwhile, xenogeneic thrombin and other coagulants may trigger immunogenic reactions [ 20 , 21 ]. On the other hand, due to the presence of anticoagulants, an additional thrombin mixing and spraying kit were further required after using a commercial PRP preparation kit, during the PRP application, especially in wound repair.…”

Section: Introductionmentioning

confidence: 99%

A Novel and Convenient Method for the Preparation and Activation of PRP without Any Additives: Temperature Controlled PRP

Miao

et al. 2018

BioMed Research International

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Platelet rich plasma (PRP) is a concentrate of autologous platelets which contain enrichment growth factors (GFs). However, the addition of exogenous anticoagulant and procoagulant may result in clinical side effects and raise the price of PRP. Herein, we report a novel method named temperature controlled PRP (t-PRP), in which exogenous additives are dispensable in the preparation and activation process. Human blood samples were processed by a two-step centrifugation process under hypothermic conditions (4°C) to obtain t-PRP and rewarming up to 37°C to activate t-PRP. Contemporary PRP (c-PRP) was processed as the control. t-PRP showed a physiological pH value between 7.46 and 7.48 and up to 6.58 ± 0.45-fold significantly higher platelet concentration than that of whole blood compared with c-PRP (4.06-fold) in the preparation process. Meanwhile, t-PRP also maintained a stable GF level between plasma and PRP. After activation, t-PRP demonstrated natural fiber scaffolding, which trapped more platelet and GFs, and exhibited a slow release and degradation rate of GFs. In addition, t-PRP exhibited the function of promoting wound healing. t-PRP is a novel and convenient method for the preparation and activation of PRP without any additives. Compared to c-PRP, t-PRP reflects more physiologic characteristics while maintaining high quality.

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Section: Introductionmentioning

confidence: 99%

A Novel and Convenient Method for the Preparation and Activation of PRP without Any Additives: Temperature Controlled PRP

Miao

et al. 2018

BioMed Research International

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“…No adverse events were observed and donor sites healed as expected. Other benefits of TT-173 reported were reduced cost compared to fibrin sealants, ease of manufacturing, and no human or animal components which decreases the risk of pathogen transmission (Rojas et al, 2017 ).…”

Section: Hemostasismentioning

confidence: 99%

Advancements in Regenerative Strategies Through the Continuum of Burn Care

et al. 2018

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Burns are caused by several mechanisms including flame, scald, chemical, electrical, and ionizing and non-ionizing radiation. Approximately half a million burn cases are registered annually, of which 40 thousand patients are hospitalized and receive definitive treatment. Burn care is very resource intensive as the treatment regimens and length of hospitalization are substantial. Burn wounds are classified based on depth as superficial (first degree), partial-thickness (second degree), or full-thickness (third degree), which determines the treatment necessary for successful healing. The goal of burn wound care is to fully restore the barrier function of the tissue as quickly as possible while minimizing infection, scarring, and contracture. The aim of this review is to highlight how tissue engineering and regenerative medicine strategies are being used to address the unique challenges of burn wound healing and define the current gaps in care for both partial- and full-thickness burn injuries. This review will present the current standard of care (SOC) and provide information on various treatment options that have been tested pre-clinically or are currently in clinical trials. Due to the complexity of burn wound healing compared to other skin injuries, burn specific treatment regimens must be developed. Recently, tissue engineering and regenerative medicine strategies have been developed to improve skin regeneration that can restore normal skin physiology and limit adverse outcomes, such as infection, delayed re-epithelialization, and scarring. Our emphasis will be centered on how current clinical and pre-clinical research of pharmacological agents, biomaterials, and cellular-based therapies can be applied throughout the continuum of burn care by targeting the stages of wound healing: hemostasis, inflammation, cell proliferation, and matrix remodeling.

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“…Even twice-daily enoxaparin prophylaxis for venous thromboembolism may increase clinically relevant bleeding after plastic and reconstructive surgery [18]. Intraoperatively, the use of topical anticoagulants is associated with increased survival of replanted digits, skin grafts, and flaps during free-tissue transfer [24]. Perhaps the oldest anticoagulant, the leech Hirudo medicinalis has proven efficacy in reducing venous congestion of skin flaps in plastic surgery, thereby preventing loss of the flap [25,26].…”

Section: Introductionmentioning

confidence: 99%

Local Pro- and Anti-Coagulation Therapy in the Plastic Surgical Patient: A Literature Review of the Evidence and Clinical Applications

et al. 2019

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The risks of systemic anti-coagulation or its reversal are well known but accepted as necessary under certain circumstances. However, particularly in the plastic surgical patient, systemic alteration to hemostasis is often unnecessary when local therapy could provide the needed adjustments. The aim of this review was to provide a summarized overview of the clinical applications of topical anti- and pro-coagulant therapy in plastic and reconstructive surgery. While not a robust field as of yet, local tranexamic acid (TXA) has shown promise in achieving hemostasis under various circumstances, hemostats are widely used to halt bleeding, and local anticoagulants such as heparin can improve flap survival. The main challenge to the advancement of local therapy is drug delivery. However, with increasingly promising innovations underway, the field will hopefully expand to the betterment of patient care.

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EHTIC study: Evaluation of a new hemostatic agent based on tissue factor in skin grafting procedures

Cited by 6 publications

References 25 publications

A Novel and Convenient Method for the Preparation and Activation of PRP without Any Additives: Temperature Controlled PRP

A Novel and Convenient Method for the Preparation and Activation of PRP without Any Additives: Temperature Controlled PRP

Advancements in Regenerative Strategies Through the Continuum of Burn Care

Local Pro- and Anti-Coagulation Therapy in the Plastic Surgical Patient: A Literature Review of the Evidence and Clinical Applications

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