Eicosanoid Production following One Bout of Exercise in Middle-Aged African American Pre- and Stage 1 Hypertensives

Abstract

Endothelial dysfunction and a sedentary lifestyle may be involved in the development of hypertension which is proliferative among middle-aged African Americans (AA). Signaling molecules derived from the oxidation of 20-carbon fatty acid molecules known as eicosanoids influence vascular tone. The relationship between aerobic fitness and eicosanoid formation following exercise in middle-aged African American hypertensives is unknown. Purpose. To determine the relationship between aerobic capacity and eicosanoid formation after a bout of moderate-intensity exercise in middle-aged AA hypertensives. Methods. Ten sedentary hypertensive AA underwent 50 min of aerobic exercise at 65% VO2max. Urine was collected for 24 hr on two occasions, prior to testing and immediately following the bout of exercise. Urinary metabolites of prostacyclin (6-keto PGF1α) and thromboxane (11-dTXB2) were measured during the day and night periods by high-performance liquid chromatography (HPLC). Results. 6-keto PGF1α levels significantly increased (P = .04) following the bout of exercise compared to the control day. There was a significant relationship (r = .49, P < .05) between 6-keto PGF1α levels and VO2max during the exercise day. Conclusion. Based on this preliminary study, there appears to be a relationship between aerobic capacity and exercise-induced 6-keto PGF1α production in middle-aged hypertensive AAs. AAs with lower VO2max had lower 6-keto PGF1α formation.

Exercise training improves endothelial function in young prehypertensives

“…It has been suggested that 6-keto-PGF 1 α is acutely elevated after a single bout of exercise and chronically elevated by long-term submaximal exercise training. 26,27 In the present study prehypertensives exhibited ∼31% reduced plasma concentration of 6-keto-PGF 1 α when compared with normotensives (Figure 2). Resistance and endurance training increased levels of 6-keto-PGF 1 α by 19% and 22%, respectively (Figure 2).…”

Eicosanoid and Eicosanoid-Related Inflammatory Mediators and Exercise Intolerance in Heart Failure with Preserved Ejection Fraction

“…Most data examining eicosanoids with exercise traits have focused on peak VO 2 , with early studies demonstrating signi cant associations of prostaglandin PGI 2 and its hydration product 6-keto-PGF1a with VO 2 max. 38,39 We too found signi cant associations of prostaglandins and prostaglandin-like metabolites with peak VO 2 , but in contrast to the early studies that demonstrated positive associations with VO 2 max, prostaglandin metabolites including 15-R PGF2a and 6ß-PGI1 were negatively associated with % predicted peak VO 2 in our sample in keeping with the hypothesis that in ammation promotes development of exercise intolerance in HFpEF. However, prostaglandins, while long regarded as pro-in ammatory metabolites, are known to exert additional anti-in ammatory and vasodilatory effects that are context dependent and may explain the discordant ndings.…”

MS-based targeted metabolomics of eicosanoids and other oxylipins: Analytical and inter-individual variabilities

“…However, short term or long term controlled dietary interventions have reported significant modulation of systemic COX-and LOX-derived oxylipin levels following intake of polyphenol-rich foods in healthy humans (138,139) even though interindividual variability sometimes hamper such demonstration (140). Other general life style habits such as smoking (141,142), alcohol consumption (143) or physical activity (144,145) can also influence systemic oxylipin patterns through an increased inflammatory state caused by the combined effects of inhaled mediators and/or oxidative stress.…”