Eicosanoids and Keratinocytes in Wound Healing

Sivamani, Raja K

doi:10.1089/wound.2014.0523

Cited by 32 publications

(26 citation statements)

References 49 publications

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“…Reepithelialization plays a key role during the wound healing and can be characterized by keratinocytes migration or proliferation, or both processes over the affected area. [41,42] To evaluate whether PBM is able to speed up the wound closure, we performed an in vitro wound model monitoring the wound closure at 24, 48 and 72 hours after scratch injury. This assay is a well-established method for investigating keratinocyte function and has been used to measure cell migration in response to a scratch injury that can mimic chronic wounds and also the skin excision after surgery.…”

Section: Pbm-induced Metabolic Activation Of Hacat Cellsmentioning

confidence: 99%

Photobiomodulation therapy promotes in vitro wound healing in nicastrin KO HaCaT cells

Tricarico

Zupin

Ottaviani

et al. 2018

Journal of Biophotonics

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Mutations in NCSTN gene (encoding for nicastrin protein) are associated with hidradenitis suppurativa (HS), a chronic inflammatory disease involving hair follicles. HS is clinically handled with drugs but the most severe cases are treated with surgery. Photobiomodulation (PBM) therapy, already used in the treatment of skin diseases such as acne, herpes virus lesions, ultraviolet damage, vitiligo, hypertrophic scar, keloid, burn, psoriasis and diabetic chronic wounds, could be beneficial as an adjuvant supportive treatment to promote and foster the healing process after skin excision in HS. The effects of PBM therapy in promoting the wound closure are evaluated in a HaCaT cells NCSTN-/-, assessing cell metabolism, migration rate, proliferation and cell cycle progression. In our experimental model, PBM exerts a potent action on metabolism of mutated keratinocytes, incrementing adenosine triphosphate (ATP) production at 2 hours, while after 24 hours an increase of metabolism with a decrement of intracellular ATP levels were recorded. Moreover, PBM speeds up the wound closure, inducing cells' migration without affecting their proliferation.Based on our findings, we suggest the use of PBM in HS patients, who undergo major surgery with large skin excision.

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Section: Pbm-induced Metabolic Activation Of Hacat Cellsmentioning

confidence: 99%

Photobiomodulation therapy promotes in vitro wound healing in nicastrin KO HaCaT cells

Tricarico

Zupin

Ottaviani

et al. 2018

Journal of Biophotonics

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“…Wound healing is divided into inflammatory, proliferative, and remodeling phases [ 1 ] that result in scar formation. Scarring involves recruitment of cells, growth factors, cytokines, and eicosanoids along with the release of enzymes to elicit extracellular matrix formation [ 1 , 2 ]. Pathological alterations of each phase are related to wound exacerbation or an inefficient healing process [ 3 ].…”

Section: Introductionmentioning

confidence: 99%

Lipoxin A4 encapsulated in PLGA microparticles accelerates wound healing of skin ulcers

et al. 2017

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Lipoxin A4 (LXA4) is involved in the resolution of inflammation and wound healing; however, it is extremely unstable. Thus, to preserve its biological activities and confer stability, we encapsulated LXA4 in poly-lactic-co-glycolic acid (PLGA) microparticles (LXA4-MS) and assessed its application in treating dorsal rat skin lesions. Ulcers were sealed with fibrin adhesive and treated with either LXA4-MS, unloaded microparticles (Un-MS), soluble LXA4, or PBS/glue (vehicle). All groups were compared at 0, 2, 7, and 14 days post-lesions. Our results revealed that LXA4-MS accelerated wound healing from day 7 and reduced initial ulcer diameters by 80%. Soluble LXA4, Un-MS, or PBS closed wounds by 60%, 45%, and 39%, respectively. LXA4-MS reduced IL-1β and TNF-α, but increased TGF-β, collagen deposition, and the number of blood vessels. Compared to other treatments, LXA4-MS reduced inflammatory cell numbers, myeloperoxidase (MPO) concentration, and metalloproteinase-8 (MMP8) mRNA in scar tissue, indicating decreased neutrophil chemotaxis. In addition, LXA4-MS treatment increased macrophages and IL-4, suggesting a positive impact on wound healing. Finally, we demonstrated that WRW4, a selective LXA4 receptor (ALX) antagonist, reversed healing by 50%, indicating that LXA4 must interact with ALX to induce wound healing. Our results show that LXA4-MS could be used as a pharmaceutical formulation for the treatment of skin ulcers.

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“…Platelet aggregation contributes to the development of atherosclerosis and acute platelet thrombus formation, followed by artery embolization. In the case of inflammation, arachidonic acid is released and metabolized by platelets to form prostaglandin, endoperoxides and thromboxane A 2 , leading to platelet activation and aggregation (gadek-Michalska et al 2013, Sivamani 2014. Thus, it is necessary to evaluate natural extracts or isolated active principles capable of inhibiting enzymes that act in the blood clotting cascade, such as thrombin, as well as in platelet aggregation, such as phospholipases A 2 and proteases.…”

Section: Resultsmentioning

confidence: 99%

Jabuticaba (Plinia jaboticaba) skin extracts as inhibitors of phospholipases A2 and proteases

Marques

Braga

César

et al. 2019

An. Acad. Bras. Ciênc.

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The phenolic extracts of jabuticaba skin flour (JSF) were characterized by HPLC, and evaluated for their modulating action upon phospholipases A 2 and proteases of snake venom, aiming at their possible use in the treatment of the various diseases associated with the action of venom toxins. Two types of extracts were prepared from JSF: aqueous and methanolic. These extracts, evaluated at different ratios, (venom: extract, m/m), significantly inhibited the phospholipase activity induced by the venom of Bothrops moojeni and Crotalus durissus terrificus, except for Bothrops atrox venom. The greatest hemolysis inhibitory action was observed for the methanolic extract, when incubated with venoms of B. moojeni and C. durissus terrificus, with inhibitions between 21 and 100%. Thrombolysis induced by venoms of B. moojeni and C. durissus terrificus was inhibited by both extracts, ranging from 32 to 83% and 51 to 83% for the aqueous and methanolic extracts, respectively. Both extracts extended coagulation time, induced by the venoms of B. moojeni and Lachesis muta muta. Inhibitory actions are related to phenolic compounds, such as gallic, syringic and p-coumaric acids, besides catechin, epigallocatechin gallate, epicatechin; resveratrol and quercetin, present in the extracts of jabuticaba skin flour, confirming their potential for nutraceutical use.

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Eicosanoids and Keratinocytes in Wound Healing

Cited by 32 publications

References 49 publications

Photobiomodulation therapy promotes in vitro wound healing in nicastrin KO HaCaT cells

Photobiomodulation therapy promotes in vitro wound healing in nicastrin KO HaCaT cells

Lipoxin A4 encapsulated in PLGA microparticles accelerates wound healing of skin ulcers

Jabuticaba (Plinia jaboticaba) skin extracts as inhibitors of phospholipases A2 and proteases

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