Eicosanoids in Exhaled Breath Condensate and BAL Fluid of Patients With Sarcoidosis

Piotrowski, Wojciech J.; Antczak, Adam; Marczak, Jerzy; Nawrocka, Agnieszka; Kurmanowska, Zofia; Górski, Paweł

doi:10.1378/chest.07-0215

Cited by 43 publications

(49 citation statements)

References 34 publications

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“…However, sarcoidosis patients with active disease as measured by BAL lymphocytes of >18% (lymphocyte alveolitis) had significantly higher levels of 8-isoprostanes in both BALF and EBC compared to nonactive sarcoid patients. This is consistent with the observation of Psathakis et al [13] and Piotrowski [14] who found that 8-isoprostane is increased in EBC from patients with active sarcoidosis and correlates with serum angiotensin-converting enzyme (sACE) [13], thus implicating the measurement of 8-isoprostane as an index of disease activity.…”

Section: Discussionsupporting

confidence: 89%

Correlation between Eicosanoids in Bronchoalveolar Lavage Fluid and in Exhaled Breath Condensate

et al. 2011

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Abstract. Exhaled breath condensate (EBC) has been increasingly used as a new and non-invasive method to study airway inflammation. In this study we have compared the concentrations of lipid mediators in EBC with concentrations in bronchoalveolar lavage fluid (BALF). We included 37 patients undergoing bronchoscopy (12 sarcoidosis, 12 COPD, 6 lung cancer, 5 chronic cough, 1 Wegener's granulomatosis, 1 sclerodermia). Patients were not allowed to have exacerbation or any change in concomitant medication for at least 4 weeks prior to the study. In all patients, EBC was collected immediately prior to the bronchoscopy. The levels of cys-LTs, LTB4, 8-isoprostane were significantly higher in BALF compared to EBC (p < 0.0001, p < 0.001, p < 0.0001 for cys-LTs, LTB4, 8-isoprostane respectively). Moreover, there was a strong positive correlation between both leukotriene B4 and 8-isoprostane in BALF and EBC (r = 0.53 and r = 0.79, p < 0.01, respectively) in patients with sarcoidosis and COPD but there was no correlation between eicosanoids BALF and EBC in patients with chronic cough and lung cancer. This is the first study to compare EBC and BALF in different lung diseases which demonstrated significant correlations between the levels of eicosanoids in BALF and EBC in patients with COPD and sarcoidosis. EBC may be useful in measuring inflammation in several inflammatory lung diseases.

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Section: Discussionsupporting

confidence: 89%

Correlation between Eicosanoids in Bronchoalveolar Lavage Fluid and in Exhaled Breath Condensate

et al. 2011

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“…Therefore, the concentration of lipids mediators is the highest in bronchus supplying the lobe with the lung cancer [21] and is much smaller in EBC due to the significant dilution of the sample [22,29]. Nevertheless, although concentration of cys-LTs were higher in BALf, Akiyama found significant positive correlation with cys-LTs levels in EBC of patients with idiopathic pulmonary fibrosis [29] whereas Piotrowski demonstrated such positive correlation for 8-isoprostane and LTB4 in patients with sarcoidosis [4]. Similarly, in our study, the concentration of LTB4, cys-LTs and 8-isporostane may reflect their concentration in BALf in patients with diagnosed NSCLC.…”

Section: Discussionmentioning

confidence: 97%

“…Various gases and non-volatile compounds have been detected and studied in the EBC that may reflect the concentrations within the extracellular epithelial lining fluid in the airways. Biomarkers found in EBC were used to monitor asthma [1,2], COPD [1] and other inflammatory diseases [4,14] while in patients with lung cancer were poorly investigated.…”

Section: Discussionmentioning

confidence: 99%

“…The collection of exhaled breath condensate (EBC) is an easy to perform, non-invasive and repeatable method that samples volatile and non-volatile substances directly from the respiratory tract [1,2]. The method offered a new insight into the pathology of airways inflammation [1,[3][4][5] however, in lung cancer the usefulness of EBC was poorly investigated.…”

Section: Introductionmentioning

confidence: 99%

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Eicosanoids in Exhaled Breath Condensate and Bronchoalveolar Lavage Fluid of Patients with Primary Lung Cancer

2012

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Abstract. Although eicosanoids are involved in lung carcinogenesis they were poorly investigated in exhaled breath condensate (EBC) and bronchoalveolar lavage fluid (BALf) in patients with primary lung cancer. In this study 17 patients with diagnosed non-small cell lung cancer, 10 healthy smokers and 12 healthy nonsmokers were included. The levels of cys-LTs, 8-isoprostane, LTB4 and PGE2 were measured before any treatment in the EBC of all patients and in BALf of patients with lung cancer by enzyme linked immunosorbent assay. 8-isoprostane, LTB4, cys-LTs and PGE2 were detectable in the EBC and BALf. There were no significant differences between healthy smokers and nonsmokers in concentrations of all measured mediators. Compared with both healthy controls, patients with diagnosed lung cancer displayed higher concentrations of cys-LTs (p < 0.05) and LTB4 (p < 0.05) in EBC. In patients with lung cancer, the mean concentrations of all measured mediators were significantly higher in BALf compared with EBC and there was a significant, positive correlation between concentration of cys-LTs, LTB4 and 8-isoprostane in BALf and their concentrations in the EBC (r = 0.64, p < 0.05, r = 0.59, p < 0.05, r = 0.53, p < 0.05 respectively). Since cys-LT, LTB4 and 8-isoprostane concentrations in EBC from patients with lung cancer reflect their concentrations in BALf, they may serve as a possible non-invasive method to monitor the disease and to assess the effectiveness of therapy.

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“…The most frequently used biological materials are serum and BAL fluid, but some new possibilities emerged recently, for instance, exhaled breath condensate (EBC) analysis (Piotrowski et al, 2007;Psathakis et al, 2004). These markers include substances that are directly produced by granuloma cells (angiotensin converting enzyme, ACE) or that result from their metabolic activity (increased serum and urinary calcium due to increased rate of hydroxylation of vitamin D), or acute phase reactants (CRP), or various immunological markers involved in initiation and propagation of granulomatous inflammation (cytokines, soluble receptors, lipid peroxidation products, other mediators).…”

Section: Laboratory Markersmentioning

confidence: 99%

Prognostic Factors in Sarcoidosis

Piotrowski¹

2011

Sarcoidosis Diagnosis and Management

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Eicosanoids in Exhaled Breath Condensate and BAL Fluid of Patients With Sarcoidosis

Cited by 43 publications

References 34 publications

Correlation between Eicosanoids in Bronchoalveolar Lavage Fluid and in Exhaled Breath Condensate

Correlation between Eicosanoids in Bronchoalveolar Lavage Fluid and in Exhaled Breath Condensate

Eicosanoids in Exhaled Breath Condensate and Bronchoalveolar Lavage Fluid of Patients with Primary Lung Cancer

Prognostic Factors in Sarcoidosis

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