Eicosapolyenoic fatty acids alter oxylipin gene expression and fatty acid hydroperoxide profiles in tomato and pepper roots

Dye, Sara M.; Yang, Jun; Bostock, Richard M.

doi:10.1016/j.pmpp.2019.101444

Cited by 12 publications

(23 citation statements)

References 53 publications

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“…Although there is striking overlap between AA/ANE and some MAMP-induced immune responses, there are also distinct differences in how AA/ANE potentially interact with immune signaling and defense. A previous gene expression study in tomato revealed AA-root treatment strongly induces local and systemic expression of several key oxylipin pathway genes (Dye, 2020). Here we show upregulation of the same subset of genes in response to ANE root treatment.…”

Section: Discussionmentioning

confidence: 99%

“…Fatty acid sodium salts (Na-FA; Nu-Chek Prep, Elysian, MN) were prepared and stored as previously described (Dye, 2020). A proprietary formulation of A. nodosum extract (ANE; APH-1011-00, Acadian Seaplants, Ltd, Nova Scotia, Canada) was diluted with deionized water (diH 2 O) to a 10% working concentration, which was used to prepare treatment dilutions.…”

Section: Methodsmentioning

confidence: 99%

“…For root/crown disease assays, P. capsici isolate PWB-53 (Hensel) was used for inoculation. Tomato seedlings were individually inoculated with 5 mL of zoospore suspension (0.5×10 4 per mL) as described in (Dye, 2020). Seventy-two hours post inoculation (hpi), disease incidence was rated on the basis of seedling collapse at the crown.…”

Section: Methodsmentioning

confidence: 99%

“…Using the standardized starting concentration, the overall motility status of the replicate, and the sum of encysted and vibrating zoospores and the number of lysed zoospores was calculated. Pathogen cultures were maintained and zoospore suspensions prepared as previously described (Dye, 2020) Literature cited Ali, N, Ramkissoon, A, Ramsubhag, A, and Jayaraj, J. (2016).…”

Section: Ane Zoospore Motility Assaymentioning

confidence: 99%

“…As the predominant polyunsaturated fatty acid in A. nodosum , AA may contribute to ANE’s biological activity. The ability of these EPs to induce resistance to diverse pathogens and trigger phytoalexin accumulation, lignin, reactive oxygen species, and programmed cell death has been shown in solanaceous and other plant families (Araceli et al, 2007; Bostock et al, 1981; Cook et al, 2018; Dye, 2020; Knight et al, 2001).…”

Section: Introductionmentioning

confidence: 99%

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Overlapping local and systemic defense induced by an oomycete fatty acid MAMP and brown seaweed extract in tomato

Lewis

Stevens

Little

et al. 2022

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Eicosapolyenoic fatty acids are integral components of oomycete pathogens that can act as microbe-associated molecular patterns (MAMPs) to induce disease resistance in plants. Defense inducing eicosapolyenoic fatty acids include arachidonic (AA) and eicosapentaenoic acids, and are strong elicitors in solanaceous plants with bioactivity in other plant families. Similarly, extracts of the brown seaweed, Ascophyllum nodosum, used in sustainable agriculture as a biostimulant of plant growth, may also induce disease resistance. A. nodosum, similar to other macroalgae, is rich in eicosapolyenoic fatty acids, which comprise as much as 25% of total fatty acid composition. We investigated the response of roots and leaves from AA or a commercial A. nodosum extract (ANE) on root-treated tomatoes via RNA sequencing, phytohormone profiling, and disease assays. AA and ANE significantly altered transcriptional profiles relative to control plants, inducing numerous defense-related genes with both substantial overlap as well as differences in gene expression patterns. Root treatment with AA and, to a lesser extent, ANE also altered both salicylic acid and jasmonic acid levels while inducing local and systemic resistance to oomycete and bacterial pathogen challenge. Thus, our study highlights overlap in both local and systemic defense induced by AA and ANE, with potential for inducing broad-spectrum resistance against pathogens.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Ane Zoospore Motility Assaymentioning

confidence: 99%