eIF4A RNA Helicase Associates with Cyclin-Dependent Protein Kinase A in Proliferating Cells and Is Modulated by Phosphorylation

Bush, Maxwell S.; Pierrat, Olivier A.; Nibau, Cândida; Mikitová, Veronika; Zheng, Tao; Corke, Fiona; Vlachonasios, Konstantinos E.; Mayberry, Laura K.; Browning, Karen S.; Doonan, John H.

doi:10.1104/pp.16.00435

Cited by 26 publications

(32 citation statements)

References 70 publications

(104 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The helicase eIF4A is also known to be phosphorylated in response to heat and hypoxia (Webster et al ., ; Le et al ., ). It has recently been found that the phosphorylation of eIF4A in proliferating cells is mediated by CYCLIN‐DEPENDENT PROTEIN KINASE A (CDKA), and the results from this research suggest that this phosphorylation downregulates the activity of eIF4A, and therefore affects translation negatively (Bush et al ., ).…”

Section: Global Changes In Translationmentioning

confidence: 97%

Translation regulation in plants: an interesting past, an exciting present and a promising future

2017

View full text Add to dashboard Cite

SUMMARYChanges in gene expression are at the core of most biological processes, from cell differentiation to organ development, including the adaptation of the whole organism to the ever-changing environment. Although the central role of transcriptional regulation is solidly established and the general mechanisms involved in this type of regulation are relatively well understood, it is clear that regulation at a translational level also plays an essential role in modulating gene expression. Despite the large number of examples illustrating the critical role played by translational regulation in determining the expression levels of a gene, our understanding of the molecular mechanisms behind such types of regulation has been slow to emerge. With the recent development of high-throughput approaches to map and quantify different critical parameters affecting translation, such as RNA structure, protein-RNA interactions and ribosome occupancy at the genome level, a renewed enthusiasm toward studying translation regulation is warranted. The use of these new powerful technologies in well-established and uncharacterized translation-dependent processes holds the promise to decipher the likely complex and diverse, but also fascinating, mechanisms behind the regulation of translation.

show abstract

Section: Global Changes In Translationmentioning

confidence: 97%

Translation regulation in plants: an interesting past, an exciting present and a promising future

2017

View full text Add to dashboard Cite

show abstract

“…This interaction is also a rate limiting step ensuring differential translation (Bush et al, 2015). In the Arabidopsis genome, two genes encode eIF4A, EIF4A1 and EIF4A2 , but only EIF4A1 knock-out mutants exhibit altered growth and cell size in a cell type-specific manner (Bush et al, 2015; Bush et al, 2016). These growth and cell size defects are partly similar to TaMAB2 -overexpression effects supporting the conclusion that eIF4A, which is highly conserved in eukaryotes (Bush et al, 2015), is potentially a TaMAB2 substrate.…”

Section: Discussionmentioning

confidence: 99%

The MATH-BTB Protein TaMAB2 Accumulates in Ubiquitin-Containing Foci and Interacts With the Translation Initiation Machinery in Arabidopsis

et al. 2019

View full text Add to dashboard Cite

MATH-BTB proteins are known to act as substrate-specific adaptors of CUL3-based E3 ligases in the ubiquitin proteasome pathway. Their BTB domain binds to CUL3 scaffold proteins and the less conserved MATH domain targets a highly diverse collection of substrate proteins to promote their ubiquitination and subsequent degradation. In plants, a significant expansion of the MATH-BTB family occurred in the grasses. Here, we report analysis of TaMAB2, a MATH-BTB protein transiently expressed at the onset of embryogenesis in wheat. Due to difficulties in studying its role in zygotes and early embryos, we have overexpressed TaMAB2 in Arabidopsis to generate gain-of-function mutants and to elucidate interaction partners and substrates. Overexpression plants showed severe growth defects as well as disorganization of microtubule bundles indicating that TaMAB2 interacts with substrates in Arabidopsis. In tobacco BY-2 cells, TaMAB2 showed a microtubule and ubiquitin-associated cytoplasmic localization pattern in form of foci. Its direct interaction with CUL3 suggests functions in targeting specific substrates for ubiquitin-dependent degradation. Although direct interactions with tubulin could not be confimed, tandem affinity purification of TaMAB2 interactors point towards cytoskeletal proteins including tubulin and actin as well as the translation initiation machinery. The idenification of various subunits of eucaryotic translation initiation factors eIF3 and eIF4 as TaMAB2 interactors indicate regulation of translation initiation as a major function during onset of embryogenesis in plants.

show abstract

“…TOR kinase additionally signals to a variety of translation-relevant proteins including MRFs, which are partners of eIF4A helicase, the RP eS6, and the inhibitor of Pol III, MAF1 (Ahn et al, 2019;Bush et al, 2016;D. H. Lee et al, 2017).…”

Section: Tor Kinase Signalingmentioning

confidence: 99%

Translational gene regulation in plants: A green new deal

2020

View full text Add to dashboard Cite

The molecular machinery for protein synthesis is profoundly similar between plants and other eukaryotes. Mechanisms of translational gene regulation are embedded into the broader network of RNA-level processes including RNA quality control and RNA turnover. However, over eons of their separate history, plants acquired new components, dropped others, and generally evolved an alternate way of making the parts list of protein synthesis work. Research over the past 5 years has unveiled how plants utilize translational control to defend themselves against viruses, regulate translation in response to metabolites, and reversibly adjust translation to a wide variety of environmental parameters. Moreover, during seed and pollen development plants make use of RNA granules and other translational controls to underpin developmental transitions between quiescent and metabolically active stages. The economics of resource allocation over the daily light-dark cycle also include controls over cellular protein synthesis. Important new insights into translational control on cytosolic ribosomes continue to emerge from studies of translational control mechanisms in viruses. Finally, sketches of coherent signaling pathways that connect external stimuli with a translational response are emerging, anchored in part around TOR and GCN2 kinase signaling networks. These again reveal some mechanisms that are familiar and others that are different from other eukaryotes, motivating deeper studies on translational control in plants.

show abstract

eIF4A RNA Helicase Associates with Cyclin-Dependent Protein Kinase A in Proliferating Cells and Is Modulated by Phosphorylation

Cited by 26 publications

References 70 publications

Translation regulation in plants: an interesting past, an exciting present and a promising future

Translation regulation in plants: an interesting past, an exciting present and a promising future

The MATH-BTB Protein TaMAB2 Accumulates in Ubiquitin-Containing Foci and Interacts With the Translation Initiation Machinery in Arabidopsis

Translational gene regulation in plants: A green new deal

Contact Info

Product

Resources

About