Let > 2 be an integer and T = {1, 2, . . . , }. We show the existence of the principal eigenvalues of linear periodic eigenvalue problem −Δ 2 ( − 1) + ( ) ( ) = ( ) ( ), ∈ T, (0) = ( ), (1) = ( + 1), and we determine the sign of the corresponding eigenfunctions, where is a parameter, ( ) ≥ 0 and ( ) ̸ ≡ 0 in T, and the weight function changes its sign in T. As an application of our spectrum results, we use the global bifurcation theory to study the existence of positive solutions for the corresponding nonlinear problem.