Eighteen new oleaginous yeast species

Abstract

Of 1600 known species of yeasts, about 70 are known to be oleaginous, defined as being able to accumulate over 20 % intracellular lipids. These yeasts have value for fundamental and applied research. A survey of yeasts from the Phaff Yeast Culture Collection, University of California Davis was performed to identify additional oleaginous species within the Basidiomycota phylum. Fifty-nine strains belonging to 34 species were grown in lipid inducing media, and total cell mass, lipid yield and triacylglycerol profiles were determined. Thirty-two species accumulated at least 20 % lipid and 25 species accumulated over 40 % lipid by dry weight. Eighteen of these species were not previously reported to be oleaginous. Triacylglycerol profiles were suitable for biodiesel production. These results greatly expand the number of known oleaginous yeast species, and reveal the wealth of natural diversity of triacylglycerol profiles within wild-type oleaginous Basidiomycetes.

The Oleaginous Yeast Meyerozyma guilliermondii BI281A as a New Potential Biodiesel Feedstock: Selection and Lipid Production Optimization

“…Yeasts are a promising source of microbial oil (Poli et al, 2013 , 2014b ; Sitepu et al, 2014 ), since these microorganisms can accumulate up to 70% of their dry weight as lipids (Angerbauer et al, 2008 ). Although several oleaginous yeasts have been described in literature as potential producers of oil as raw material for biodiesel (Ageitos et al, 2011 ; Duarte et al, 2013 ; Poli et al, 2013 , 2014a ; Garay et al, 2016 ), biofuel production from yeast oil is not yet a reality, mainly due to the high cost of microbial oil production. Therefore, efforts are necessary to assess new lipid-producing yeasts and to enhance their lipid production using low cost raw materials to turn yeast oil applications cost-effective.…”

Use of the “DNAChecker” Algorithm for Improving Bioinformatics Research

“…However, we utilize multiple N's in the beginning and the end of the sequence to provide the starting and ending points for the program to read the sequence. This creates a suitable measure of the significantly more important and neater sequence, which is improved than the earlier efforts [15][16][17].…”

A comprehensive genome-scale model forRhodosporidium toruloidesIFO0880 accounting for functional genomics and phenotypic data

“…In contrast, the growth 6 associated maintenance (GAM) was condition-dependent with a value of 140.98 mmol gDW -1 7 under carbon limited and 276.37 mmol gDW -1 under nitrogen limited conditions. In yeast 7.6, 8 NGAM is not modeled (though an earlier S. cerevisiae model [62] reported an NGAM value of 1 9 mmol gDW -1 ) and the GAM value is 59.28 mmol gDW -1 . The GAM value quantifies growth-10 associated energy costs that are not captured in the biomass equation, alluding to higher energy 11 demands for R. toruloides growth compared to S. cerevisiae.…”

“…metabolite pool, the coefficients of twelve cofactors and prosthetic groups were set to a small 15 number of 10 -4 so as to impose a biosynthesis requirement on the in silico model, resulting in 16 0.06% of the total biomass by weight. A similar measure was also adopted for the biomass 17 reactions in other models such as those for S. cerevisiae models, including yeast 7.6 [62]. as these ions are known to be essential (Supplementary Materials 2).…”