Eignung verschiedener15N-Verbindungen als Tracer für Körpermarkierung und für Untersuchungen des Protein- und Aminosäuremetabolismus

Krawielitzki, K.; Schadereit, R.; Herrmann, U.

doi:10.1080/10256018608623665

Isotopenpraxis Isotopes in Environmental and Health Studies

1986

DOI: 10.1080/10256018608623665

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Eignung verschiedener¹⁵N-Verbindungen als Tracer für Körpermarkierung und für Untersuchungen des Protein- und Aminosäuremetabolismus

K. Krawielitzki¹,

R. Schadereit²,

U. Herrmann³

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Cited by 4 publications

(1 citation statement)

References 30 publications

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“…But the question how exactly the metabolism of the chosen tracer amino acid reflects the metabolism of the whole body N, is of course relevant (Krawielitzki et al, 1986).…”

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confidence: 99%

Estimation of protein and amino acid metabolism using [15N] and [14C] labeled tracer amino acids

Krawielitzki¹,

Kreienbring²,

Schadereit³

et al. 1996

J. Anim. Feed Sci.

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The possibilities of estimating parameters for whole body protein metabolism (synthesis and breakdown) by the help of the end product method and a compartment model using l5 N-and/or 14 C labeled amino acids (AA) as tracers are demonstrated. First, the fundamentals of the method, the choice of tracer AA and the determinable parameters are described. The influence of endogenous and exogenous factors on protein synthesis and breakdown is demonstrated using selected results of tracer experiments on rats. Differences relating to animal species and the effect of age, body weight, protein intake, ^-agonist administration and training, are shown in detail.The main conclusions are: -the tracer method in combination with modeling provides parameters of protein metabolism on intact animals; -the estimated data are relative values and they reflect the main processes of protein and/or AA metabolism with sufficient accuracy; -the method makes it possible to assess the influence of different endogenous and exogenous factors on the protein synthesis rate.KEY WORDS: amino acid, protein, metabolism model, isotope tracer 188 KRAWIELITZKI K. ET AL.

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“…But the question how exactly the metabolism of the chosen tracer amino acid reflects the metabolism of the whole body N, is of course relevant (Krawielitzki et al, 1986).…”

mentioning

confidence: 99%

Estimation of protein and amino acid metabolism using [15N] and [14C] labeled tracer amino acids

Krawielitzki¹,

Kreienbring²,

Schadereit³

et al. 1996

J. Anim. Feed Sci.

Self Cite

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The Influence of Feeding Frequency on Nitrogen Turnover and Whole Body Protein Synthesis in Adult Rats (Estimation using [¹⁴C]- and [¹⁵N]- Labelled Leucine)

Krawielitzki¹,

Schreurs

Kreienbring³

et al. 1997

Isotopes in Environmental and Health Studies

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Untersuchungen zur Dosisabhängigkeit der¹⁵N-Inkorporation in Organproteine neugeborener Ratten nach Pulsmarkierung mit verschiedenen Tracern

Wutzke

Zhukova²,

Sorokina³

et al. 1987

Archiv für Tierernaehrung

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A short-chain 15N-peptide mixture characterized by an average chain length of 2.3 was obtained when 15N-labeled yeast protein has hydrolyzed enzymatically by thermitase from Thermoactinomyces vulgaris. Fifteen newborn Wistar-rats were given a single pulse of [15N]glycine. [15N]H4Cl and [15N]yeast protein-thermitasehydrolysate (YPTH) in a dosage of 50 mg 15N excess kg-1 by gastric tube. In comparison with [15N]glycine the 15N-incorporation rates of brain, muscle and liver were approximately 150% higher after [15N]YPTH-application. Uniform labeling, high 15N-enrichment, almost complete absorption, avoidance of imbalances and the low price make this tracer substance superior to other tracers conventionally used for organ labeling.

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Eignung verschiedener¹⁵N-Verbindungen als Tracer für Körpermarkierung und für Untersuchungen des Protein- und Aminosäuremetabolismus

Cited by 4 publications

References 30 publications

Estimation of protein and amino acid metabolism using [<sup>15</sup>N] and [<sup>14</sup>C] labeled tracer amino acids

Estimation of protein and amino acid metabolism using [<sup>15</sup>N] and [<sup>14</sup>C] labeled tracer amino acids

The Influence of Feeding Frequency on Nitrogen Turnover and Whole Body Protein Synthesis in Adult Rats (Estimation using [¹⁴C]- and [¹⁵N]- Labelled Leucine)

Untersuchungen zur Dosisabhängigkeit der¹⁵N-Inkorporation in Organproteine neugeborener Ratten nach Pulsmarkierung mit verschiedenen Tracern

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Eignung verschiedener15N-Verbindungen als Tracer für Körpermarkierung und für Untersuchungen des Protein- und Aminosäuremetabolismus

Cited by 4 publications

References 30 publications

Estimation of protein and amino acid metabolism using [<sup>15</sup>N] and [<sup>14</sup>C] labeled tracer amino acids

Estimation of protein and amino acid metabolism using [<sup>15</sup>N] and [<sup>14</sup>C] labeled tracer amino acids

The Influence of Feeding Frequency on Nitrogen Turnover and Whole Body Protein Synthesis in Adult Rats (Estimation using [14C]- and [15N]- Labelled Leucine)

Untersuchungen zur Dosisabhängigkeit der15N-Inkorporation in Organproteine neugeborener Ratten nach Pulsmarkierung mit verschiedenen Tracern

Contact Info

Product

Resources

About

Eignung verschiedener¹⁵N-Verbindungen als Tracer für Körpermarkierung und für Untersuchungen des Protein- und Aminosäuremetabolismus

The Influence of Feeding Frequency on Nitrogen Turnover and Whole Body Protein Synthesis in Adult Rats (Estimation using [¹⁴C]- and [¹⁵N]- Labelled Leucine)

Untersuchungen zur Dosisabhängigkeit der¹⁵N-Inkorporation in Organproteine neugeborener Ratten nach Pulsmarkierung mit verschiedenen Tracern