2021
DOI: 10.3389/fcimb.2021.703413
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Eimeria bovis Macromeront Formation Induces Glycolytic Responses and Mitochondrial Changes in Primary Host Endothelial Cells

Abstract: Eimeria bovis is an intracellular apicomplexan parasite that causes considerable economic losses in the cattle industry worldwide. During the first merogony, E. bovis forms large macromeronts with >140,000 merozoites I in host endothelial cells. Because this is a high-energy demanding process, E. bovis exploits the host cellular metabolism to fulfill its metabolic requirements. We here analyzed the carbohydrate-related energetic metabolism of E. bovis–infected primary bovine umbilical vein endothelial c… Show more

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Cited by 3 publications
(5 citation statements)
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“…By the combination of WB- and FACS-based analyses, we here demonstrated that N. caninum- infected host endothelial cells were arrested at the DNA synthesis stage when harboring immature meronts with no more than 8 tachyzoites onwards. One plausible explanation for cell cycle arrest in S-phase is that during this phase not only protein-, amino sugar- and pentose phosphate-synthesis are up-regulated but also metabolic pathways, such as glycolysis/gluconeogenesis and nucleotide sugar metabolism, all of them fundamental for both, host cell maintenance and intracellular parasite proliferation ( Regidor-Cerrillo et al, 2020 ; Velásquez et al, 2021 ; Vélez et al, 2021 ). Given that tachyzoite production is an energy- and building block-demanding process, the transient or continuous deprivation of essential molecules could indeed induce an energy-lacking status in N. caninum -infected host cells, finally leading to a block in DNA synthesis and/or mitotic process.…”
Section: Discussionunclassified
“…By the combination of WB- and FACS-based analyses, we here demonstrated that N. caninum- infected host endothelial cells were arrested at the DNA synthesis stage when harboring immature meronts with no more than 8 tachyzoites onwards. One plausible explanation for cell cycle arrest in S-phase is that during this phase not only protein-, amino sugar- and pentose phosphate-synthesis are up-regulated but also metabolic pathways, such as glycolysis/gluconeogenesis and nucleotide sugar metabolism, all of them fundamental for both, host cell maintenance and intracellular parasite proliferation ( Regidor-Cerrillo et al, 2020 ; Velásquez et al, 2021 ; Vélez et al, 2021 ). Given that tachyzoite production is an energy- and building block-demanding process, the transient or continuous deprivation of essential molecules could indeed induce an energy-lacking status in N. caninum -infected host cells, finally leading to a block in DNA synthesis and/or mitotic process.…”
Section: Discussionunclassified
“…The viability of plasmatocytes from D. suzukii L3 larvae was assessed by 3D holotomography [ 38 ]. Freshly isolated cells were incubated in an imaging medium at room temperature until more than 90% of the cells were dead.…”
Section: Resultsmentioning
confidence: 99%
“…D. suzukii robust cellular immune responses might have facilitated its rapid worldwide spread by allowing it to overcome pathogen infections in newly colonized environments [ 55 , 56 , 57 ]. Such immunological diversity can help invasive species deal with unfamiliar pathogens [ 10 , 11 , 12 , 13 , 14 , 15 , 16 , 17 , 18 , 19 , 20 , 21 , 22 , 23 , 24 , 25 , 26 , 27 , 28 , 29 , 30 , 31 , 32 , 33 , 34 , 35 , 36 , 37 , 38 , 39 , 40 , 41 , 42 , 43 , 44 , 45 , 46 , 47 , 49 , 50 , 51 , 52 , 53 , 54 , 55 , 56 , 57 , 58 ], as recently shown for the invasive harlequin ladybird Harmonia axyridis [ 48 , 55 , 56 , 57 ...…”
Section: Discussionmentioning
confidence: 99%
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“…Eimeria bovis is a species of protozoan that has the environment as an important part of its evolutionary cycle, for example, with the maintenance of its infective pre-parasitic forms (sporulated oocysts) that are deposited by means of the faeces of infected animals [8,9]. Thus, control mechanisms are always sought, both parasitic and in the environment, which is an important premise from a scienti c point of view.…”
Section: Introductionmentioning
confidence: 99%