Ein neues knospendes Bakterium aus der Ostsee

Ahrens, Renate; Moll, Georg

doi:10.1007/bf00407714

Cited by 66 publications

(55 citation statements)

References 13 publications

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“…Geodermatophilaceae are an actinobacterial family (Normand, 2006) in the order Geodermatophiliales (Sen et al, 2014) that comprises three genera: Geodermatophilus, Blastococcus and Modestobacter initially isolated from desert soils (Luedemann, 1968), sea water (Ahrens and Moll, 1970) and Antarctic regolith (Mevs et al, 2000), respectively. These three genera have a complex life cycle and produce remarkably resistant enzymes such as esterases (Essoussi et al, 2010;Jaouani et al, 2012;Normand et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

Stone-dwelling actinobacteria Blastococcus saxobsidens, Modestobacter marinus and Geodermatophilus obscurus proteogenomes

Sghaier¹,

Hezbri²,

Ghodhbane‐Gtari³

et al. 2015

The ISME Journal

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The Geodermatophilaceae are unique model systems to study the ability to thrive on or within stones and their proteogenomes (referring to the whole protein arsenal encoded by the genome) could provide important insight into their adaptation mechanisms. Here we report the detailed comparative genome analysis of Blastococcus saxobsidens (Bs), Modestobacter marinus (Mm) and Geodermatophilus obscurus (Go) isolated respectively from the interior and the surface of calcarenite stones and from desert sandy soils. The genome-scale analysis of Bs, Mm and Go illustrates how adaptation to these niches can be achieved through various strategies including 'molecular tinkering/ opportunism' as shown by the high proportion of lost, duplicated or horizontally transferred genes and ORFans. Using high-throughput discovery proteomics, the three proteomes under unstressed conditions were analyzed, highlighting the most abundant biomarkers and the main protein factors. Proteomic data corroborated previously demonstrated stone-related ecological distribution. For instance, these data showed starvation-inducible, biofilm-related and DNA-protection proteins as signatures of the microbes associated with the interior, surface and outside of stones, respectively.

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Section: Introductionmentioning

confidence: 99%

Stone-dwelling actinobacteria Blastococcus saxobsidens, Modestobacter marinus and Geodermatophilus obscurus proteogenomes

Sghaier¹,

Hezbri²,

Ghodhbane‐Gtari³

et al. 2015

The ISME Journal

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“…The genus Blastococcus was first proposed by Ahrens & Moll (1970) Lee, 2006), which were isolated from the Baltic Sea, limestone and a beach sand, respectively. In this study, we report a novel species isolated from Camptotheca acuminata, a traditional Chinese medicinal plant.…”

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confidence: 99%

Blastococcus endophyticus sp. nov., an actinobacterium isolated from Camptotheca acuminata

Zhu

Zhang

Qin

et al. 2013

International Journal of Systematic and Evolutionary Microbiology

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A novel endophytic actinobacterium, designated strain YIM 68236 T , was isolated from healthy leaves of Camptotheca acuminata. and characterized by using a polyphasic approach. Cells of this strain occurred singly, in pairs or in tetrads. It grew at 10-45 6C, at pH 5.0-8.0 (optimum pH 7.0) and in the presence of 0-3 % (w/v) NaCl. The DNA G+C content was 71.6 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain YIM 68236 T belongs to the genus Blastococcus. However, it differed from its closest relatives, Blastococcus aggregatus DSM 4725 T , Blastococcus saxobsidens DSM 44509 T and Blastococcus jejuensis DSM 19597 T in many phenotypic characteristics. Moreover, the DNA-DNA relatedness values between the novel isolate and the three above-mentioned type strains were 49.0±1.6 %, 46.1±3.2 % and 39.8±1.5 %, respectively. Based on comparative analysis of physiological and chemotaxonomic data, strain YIM 68236 T represents a novel species of the genus Blastococcus, for which the name Blastococcus endophyticus sp. nov. is proposed. The type strain is YIM 68236 T (5CCTCC AA 209045 T 5DSM 45413 T 5KCTC 19998 T ).The genus Blastococcus was first proposed by Ahrens & Moll (1970) Lee, 2006), which were isolated from the Baltic Sea, limestone and a beach sand, respectively. In this study, we report a novel species isolated from Camptotheca acuminata, a traditional Chinese medicinal plant.Healthy leaves of C. acuminata were washed in running water to remove soil particles and sterilized by the established procedure (Li et al., 2008). After being surfacesterilized, the samples were sliced into pieces, followed by plating on TWYE agar (containing 0.25 g yeast extract, 0.5 g K 2 HPO 4 and 18 g agar l 21 tap water, pH 7.2) containing nalidixic acid (25 mg l 21 ), nystatin (50 mg l 21 ) and cycloheximide (50 mg l 21 ) to repress growth of Gramreaction-negative bacteria and fungi. The plates were incubated at 28 u C for 4-6 weeks until the outgrowth of endophytic actinobacterial strains was discerned. Colonies originating from leaf segments were selected and pure cultures were obtained by repeated streaking on medium YIM 38 [10 g malt extract, 4 g yeast extract, 4 g glucose, vitamin mixture (containing 0.5 mg each of thiamine-HCl, riboflavin, niacin, pyridoxine-HCl, inositol, calcium pantothenate and p-aminobenzoic acid and 0.25 mg biotin), 20 g agar; pH 7.2] . The purified strain YIM 68236 T was maintained on tryptic soy agar (TSA) slants at 4 u C and as 20 % (v/v) glycerol suspensions at 280 u C.Extraction of genomic DNA, PCR amplification and sequencing of the 16S rRNA gene were carried out as described by Li et al. (2007). Multiple alignments with sequences of the most closely related actinobacteria were carried out using the CLUSTAL X 1.8 program (Thompson et al., 1997). Phylogenetic trees were reconstructed by the

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“…These three genera share the remarkable morphological feature of the formation of sporangia of up to 100 mm in diameter that divide in all three planes. A few years before, Ahrens & Moll (1970) isolated and described a bacterium from the Baltic sea that formed budding structures, Blastococcus aggregatus. Recently, Urzi et al (2004) published an emended description of genus Blastococcus and the genus now comprises two species, Blastococcus aggregatus and Blastococcus saxobsidens.…”

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confidence: 99%

Geodermatophilaceae fam. nov., a formal description

Normand

2006

International Journal of Systematic and Evolutionary Microbiology

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The family name ‘Geodermatophilaceae’ was first published by Normand et al. (1996) to contain the genera Geodermatophilus and Blastococcus, but a formal description and the designation of the type genus were not included, thus making the name invalid. Since then, the genus Modestobacter has been described as a member of the family ‘Geodermatophilaceae’ by Mevs et al. (2000). We hereby formally describe the family ‘Geodermatophilaceae’ which includes the recently described genus Modestobacter. The type genus of the family is Geodermatophilus.

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Ein neues knospendes Bakterium aus der Ostsee

Cited by 66 publications

References 13 publications

Stone-dwelling actinobacteria Blastococcus saxobsidens, Modestobacter marinus and Geodermatophilus obscurus proteogenomes

Stone-dwelling actinobacteria Blastococcus saxobsidens, Modestobacter marinus and Geodermatophilus obscurus proteogenomes

Blastococcus endophyticus sp. nov., an actinobacterium isolated from Camptotheca acuminata

Geodermatophilaceae fam. nov., a formal description

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