Eine automatische Methode zur Bestimmung proteolytischer Enzyme

Reisner, Walter; Uhlig, H.

doi:10.1007/bf00521708

Cited by 6 publications

(1 citation statement)

References 4 publications

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“…Several authors have recently published the automation of the method of Kunitz (2) and Hagihara (3,4) with the aid of an AutoAnalyzer. Falck (5) Reisner et al (7) states that, in general, within a day the range did not exceed + 2% at a sampling rate of 20 samples per hour. Apparently the number of samples per hour includes tile calibrating solutions; the number of unknowns per hour will be considerably lower.…”

Section: Automation: Continuous Flow System or Discrete Systemmentioning

confidence: 99%

Automated determination of protease for laundry compounds

Detmar¹,

Vogels²

1971

J Americ Oil Chem Soc

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For the determination of the activity of protease for laundry washing compounds preference is given to casein as substrate and measurement of the supernatant at 275 nm after precipitation with trichloroacetic acid. Since sampling rate and precision with the AutoAnalyzer are in this case very low, an automated system based on the principle of the mechanized test tube has been developed. The sampling rate is one sample per minute; the coefficient of variation of a single sample is 2.3% without relation to a standard solution and 1.6% if results are related to a standard solution run simultaneously. If the activity of a sample is determined in threefold, the coefficients of variation are 1.6% resp. 1.1%.

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Section: Automation: Continuous Flow System or Discrete Systemmentioning

confidence: 99%

Automated determination of protease for laundry compounds

Detmar¹,

Vogels²

1971

J Americ Oil Chem Soc

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Continuous determination of protease during the fermentation using the autoanalyzer adm 300

Lupin

Körner

1991

Acta Biotechnologica

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A method for automatic determination of proteolytic activity during fermentation is described. Therefore the autoanalyzer ADM 300 of the PRUEFGERAETEWERK MEDINGEN F.R.G. is connected to a fermenter. After filtration of the biomass, the culture filtrate is diluted and then incubated with casein solution (0.75%, p H 8.0, 55OC); low moleculare cleavage products are separated from higher molecular ones by dialysis. The absorbence is measured a t 600 nm after addition of alkaline copper tartrate and FOLIN solution. By use of different dilution rates, a range of 0 -32 units ml-1. may be measured. The coefficient of variation is 5.5.

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Eine verbesserte Methode zur automatischen Bestimmung proteolytischer Enzyme in Waschmitteln nach dem Prinzip der kontinuierlichen Durchflußanalyse

Pfeiffer

Lange

1973

Fette, Seifen, Anstrichm.

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Das Verfahren baut auf den erprobten Techniken der kontinuierlichen Durchflußanalyse auf: Caseinlösung wird kontinuierlich mit enzymhaltigen Probensegmenten bei 50° C inkubiert. Anschließend wird das nichthydrolysierte Casein mit Trichloressigsäure gefällt, abfiltriert und im Filtrat das freigesetzte Tyrosin bei alkalischem pH‐Wert und 290 nm gemessen. Durch Verwendung einer speziellen Fällungsvorrichtung wird erstmals eine Alterung des Caseinniederschlages erreicht, bevor dieser kontinuierlich abfiltriert wird. Eine synchron führende Parallelstrecke gestattet für jede Probe eine individuelle Blindwertbestimmung, wodurch die unmittelbare Automatisierung cines bereits in der Praxis erprobten manuellen Verfahrens ermöglicht wird, ohne spezielle Substrate oder Analysemethoden anwenden zu müssen.

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Eine automatische Methode zur Bestimmung proteolytischer Enzyme

Cited by 6 publications

References 4 publications

Automated determination of protease for laundry compounds

Automated determination of protease for laundry compounds

Continuous determination of protease during the fermentation using the autoanalyzer adm 300

Eine verbesserte Methode zur automatischen Bestimmung proteolytischer Enzyme in Waschmitteln nach dem Prinzip der kontinuierlichen Durchflußanalyse

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