Eine Methode der viscosit�tsmessung der perilymphe in der meerschweinchenschnecke unter physiologischen bedingungen

Dahl, Dieter; Kleinfeldt, D

doi:10.1007/bf00300285

Cited by 3 publications

(2 citation statements)

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“…The cochlear fluid is perilymph in scala vestibuli (SV) and scala tympani (ST), and endolymph in scala media (SM). Their viscosity is similar to that of water (Kleinfeldt and Dahl, 1970). The traveling wave in the cochlea is based on fluid inertia and OC stiffness, and if the fluid in the cochlea became substantially more viscous, the cochlea’s mechanical response and wave pattern will change.…”

Section: Introductionmentioning

confidence: 78%

“…The shear stress and pressure drops produced during the perfusion were calculated using the formulae introduced in section 2.6 and results are shown in Table 1. Pure perilymph (AP) is also considered here for reference, and is taken to have a viscosity similar to that of water, 0.001 Pas (Kleinfeldt and Dahl, 1970). The viscosities for HA solution with different concentration were estimated from Maleki et al (2007).…”

Section: Discussionmentioning

confidence: 99%

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Cochlear perfusion with a viscous fluid

Wang

Olson

2016

Hearing Research

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The flow of viscous fluid in the cochlea induces shear forces, which could provide benefit in clinical practice, for example to guide cochlear implant insertion or produce static pressure to the cochlear partition or wall. From a research standpoint, studying the effects of a viscous fluid in the cochlea provides data for better understanding cochlear fluid mechanics. However, cochlear perfusion with a viscous fluid may damage the cochlea. In this work we studied the physiological and anatomical effects of perfusing the cochlea with a viscous fluid. Gerbil cochleae were perfused at a rate of 2.4 μL/min with artificial perilymph (AP) and sodium hyaluronate (Healon, HA) in four different concentrations (0.0625%, 0.125%, 0.25%, 0.5%). The different HA concentrations were applied either sequentially in the same cochlea or individually in different cochleae. The perfusion fluid entered from the round window and was withdrawnfrom basal scala vestibuli, in order to perfuse the entire perilymphatic space. Compound action potentials (CAP) were measured after each perfusion. After perfusion with increasing concentrations of HA in the order of increasing viscosity, the CAP thresholds generally increased. The threshold elevation after AP and 0.0625% HA perfusion was small or almost zero, and the 0.125% HA was a borderline case, while the higher concentrations significantly elevated CAP thresholds. Histology of the cochleae perfused with the 0.0625% HA showed an intact Reissner’s membrane, while in cochleae perfused with 0.125% and 0.25% HA Reissner’s membrane (RM) was torn. Thus, the CAP threshold elevation was likely due to the broken of RM, which likely caused by the shear stress produced by the flow of the viscous fluid. Our results and analysis indicate that the cochlea can sustain, without a significant CAP threshold shift, up to a 1.5 Pa shear stress. Beside these finding, in the 0.125% and 0.25% HA perfusion cases, a temporary CAP threshold shift was observed, perhaps due to the presence and then clearance of viscous fluid within the cochlea, or to a temporary position shift of the Organ of Corti. After 0.5% HA perfusion, a short latency positive peak (P0) appeared in the CAP wavefrom. This P0 might be due to a change in the cochlea’s traveling-wave pattern, or distortion in the cochlear microphonic.

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Section: Introductionmentioning

confidence: 78%

Section: Discussionmentioning

confidence: 99%

Cochlear perfusion with a viscous fluid

Wang

Olson

2016

Hearing Research

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Tonotopic Morphometry of the Lamina Reticularis of the Guinea Pig Cochlea with Associated Microstructures and Related Mechanical Implications

Yarin

Lukashkin

Poznyakovskiy

et al. 2013

JARO

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Morphometry of the lamina reticularis of the guinea pig cochlea was performed using scanning electron microscopy. Seventy-four geometrical parameters of the lamina reticularis, the bundles of stereocilia, and individual stereocilia, in all rows of hair cells and within the individual hair cells, were measured at ten equally spaced locations along the longitudinal direction of the cochlea. Variations of the parameters versus the longitudinal coordinate were statistically analyzed and fitted with polynomials (constant, linear, or quadratic). Our data show that a unique set of geometrical parameters of inner and outer hair cells is typical for every frequency-dependent position at the lamina reticularis. Morphology of the outer hair cell structures varies more than respective parameters of the inner hair cells. Mechanical modeling using the obtained geometrical parameters provides a novel glance at the mechanical characteristics with respect to the cochlear tonotopy.

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The sensitivity of the cochlear amplifier to changes in operating conditions

Wang¹

2019

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Eine Methode der viscosit�tsmessung der perilymphe in der meerschweinchenschnecke unter physiologischen bedingungen

Cited by 3 publications

References 0 publications

Cochlear perfusion with a viscous fluid

Cochlear perfusion with a viscous fluid

Tonotopic Morphometry of the Lamina Reticularis of the Guinea Pig Cochlea with Associated Microstructures and Related Mechanical Implications

The sensitivity of the cochlear amplifier to changes in operating conditions

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