Einfluss der Morphologie auf Stofftransport und ‐umsatz in <i>Aspergillus niger</i>‐Pellets

Hille, A.; Neu, Thomas R.; Hempel, D. C.; Horn, Harald

doi:10.1002/cite.200600018

Cited by 11 publications

(7 citation statements)

References 19 publications

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“…Additionally, cultivation in loose mycelia form can often result in fouling of reactor internals, such as the sensors. Mass transport in the pellets can be one of the disadvantages of pellet morphology which might also affect the productivity [30].…”

Section: Morphology Of Penicillium Sp During Fermentationsmentioning

confidence: 99%

Applicability of a single‐use bioreactor compared to a glass bioreactor for the fermentation of filamentous fungi and evaluation of the reproducibility of growth in pellet form

Soerjawinata

Schlegel

Fuchs

et al. 2021

Engineering in Life Sciences

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The implementation of single-use technologies offers several major advantages, e.g. prevention of cross-contamination, especially when spore-forming microorganisms are present. This study investigated the application of a single-use bioreactor in batch fermentation of filamentous fungus Penicillium sp. (IBWF 040-09) from the Institute of Biotechnology and Drug Research (IBWF), which is capable of intracellular production of a protease inhibitor against parasitic proteases as a secondary metabolite. Several modifications to the SU bioreactor were suggested in this study to allow the fermentation in which the fungus forms pellets. Simultaneously, fermentations in conventional glass bioreactor were also conducted as reference. Although there are significant differences in the construction material and gassing system, the similarity of the two types of bioreactors in terms of fungal metabolic activity and the reproducibility of fermentations could be demonstrated using statistic methods. Under the selected cultivation conditions, growth rate, yield coefficient, substrate uptake rate, and formation of intracellular protease-inhibiting substance in the single-use bioreactor were similar to those in the glass bioreactor.

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Section: Morphology Of Penicillium Sp During Fermentationsmentioning

confidence: 99%

Applicability of a single‐use bioreactor compared to a glass bioreactor for the fermentation of filamentous fungi and evaluation of the reproducibility of growth in pellet form

Soerjawinata

Schlegel

Fuchs

et al. 2021

Engineering in Life Sciences

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“…A higher surface-to-volume ratio in smaller pellets and different peripheral hyphae structures are assumed to support nutrient uptake [ 56 ]. For hairy and compact pellet structures, even if pellet size remained identical, completely different oxygen consumption rates were shown [ 54 ]. Thus, the different pellet morphology of the wild type might be the reason for the observed limitations.…”

Section: Discussionmentioning

confidence: 99%

“…Besides changes in the nutrient uptake and oxygen supply [ 54 , 58 – 60 ], pellet formation leads to different grades of heterogeneity of the cells accumulated in the pellets [ 61 – 63 ]. The use of isoenzymes was seen for several pathways in the wild type strain, which might be explained by higher cell heterogeneity within the pellets.…”

Section: Discussionmentioning

confidence: 99%

Proteomic Analysis of Anti-Cancerous Scopularide Production by a Marine Microascus brevicaulis Strain and Its UV Mutant

et al. 2015

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The marine fungus Microascus brevicaulis strain LF580 is a non-model secondary metabolite producer with high yields of the two secondary metabolites scopularides A and B, which exhibit distinct activities against tumour cell lines. A mutant strain was obtained using UV mutagenesis, showing faster growth and differences in pellet formation besides higher production levels. Here, we show the first proteome study of a marine fungus. Comparative proteomics were applied to gain deeper understanding of the regulation of production and of the physiology of the wild type strain and its mutant. For this purpose, an optimised protein extraction protocol was established. In total, 4759 proteins were identified. The central metabolic pathway of strain LF580 was mapped using the KEGG pathway analysis and GO annotation. Employing iTRAQ labelling, 318 proteins were shown to be significantly regulated in the mutant strain: 189 were down- and 129 upregulated. Proteomics are a powerful tool for the understanding of regulatory aspects: The differences on proteome level could be attributed to limited nutrient availability in the wild type strain due to a strong pellet formation. This information can be applied for optimisation on strain and process level. The linkage between nutrient limitation and pellet formation in the non-model fungus M. brevicaulis is in consensus with the knowledge on model organisms like Aspergillus niger and Penicillium chrysogenum.

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“…However, in practice, pellets have an almost spherical geometry and a spherically symmetrical density distribution. Morphological investigations on equatorial sections have also shown that the filament distribution and the mass density within a pellet can vary considerably radially [25,92,135]. In addition, recent microscopic measurements confirm that the pellet density can vary significantly depending on the radius, see Figure 5.…”

Section: Fluidmechanical Models To Describe the Pellet Morphology Andmentioning

confidence: 96%

Challenges of influencing cellular morphology by morphology engineering techniques and mechanical induced stress on filamentous pellet systems—A critical review

Böl

Schrinner

Tesche

et al. 2020

Engineering in Life Sciences

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Filamentous microorganisms are main producers of organic acids, enzymes, and pharmaceutical agents such as antibiotics and other active pharmaceutical ingredients. With their complex cell morphology, ranging from dispersed mycelia to dense pellets, the cultivation is challenging. In recent years, various techniques for tailor‐made cell morphologies of filamentous microorganisms have been developed to increase product formation and have been summarised under the term morphology engineering. These techniques, namely microparticle‐enhanced cultivation, macroparticle‐enhanced cultivation, and alteration of the osmolality of the culture medium by addition of inorganic salts, the salt‐enhanced cultivation, are presented and discussed in this review. These techniques have already proven to be useful and now await further proof‐of‐concept. Furthermore, the mechanical behaviour of individual pellets is of special interest for a general understanding of pellet mechanics and the productivity of biotechnological processes with filamentous microorganisms. Correlating them with substrate uptake and finally with productivity would be a breakthrough not to be underestimated for the comprehensive characterisation of filamentous systems. So far, this research field is under‐represented. First results on filamentous pellet mechanics are discussed and important future aspects, which the filamentous expert community should deal with, will be presented and critically discussed.

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Einfluss der Morphologie auf Stofftransport und ‐umsatz in Aspergillus niger‐Pellets

Abstract: Sowohl der Stofftransport als auch der Stoffumsatz in Pilzpellets von

Cited by 11 publications

References 19 publications

Applicability of a single‐use bioreactor compared to a glass bioreactor for the fermentation of filamentous fungi and evaluation of the reproducibility of growth in pellet form

Applicability of a single‐use bioreactor compared to a glass bioreactor for the fermentation of filamentous fungi and evaluation of the reproducibility of growth in pellet form

Proteomic Analysis of Anti-Cancerous Scopularide Production by a Marine Microascus brevicaulis Strain and Its UV Mutant

Challenges of influencing cellular morphology by morphology engineering techniques and mechanical induced stress on filamentous pellet systems—A critical review

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