Einfluss der Sporulation auf die Produktion einer thermostabilen α‐Amylase in <i>Bacillus caldolyticus</i>

Brokamp, Christian; Weigel, Christoph; Jamrath, T.; Popović, Milan

doi:10.1002/cite.200650199

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Experiments In Bioreactors1

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2009

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“…This duration correlates with the time needed for sporulation reported in literature [32]. The observed sporulation might be decreased by reducing the Mn concentration in the fermentation broth [33].…”

Section: Experiments In Bioreactorssupporting

confidence: 71%

Dual feeding strategy for the production of α-amylase by Bacillus caldolyticus using complex media

et al. 2009

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In this study, the objective was to investigate an exponential feeding strategy for fed-batch production of thermostable alpha-amylase (E.C. 3.2.1.1.) from the Bacillus caldolyticus (DSM405). The parameters for establishing compositions of feed media and feeding rate were obtained by statistical analysis of batch and continuous shake flask experiments. These parameters were casitone to starch ratio of 2.67g(casitone)g(starch)(-1), maintenance coefficient 0.174g(casitone)g(DW)(-1)h(-1), cell yield 0.62g(DW)g(casitone)(-1) and mu(opt)=0.2h(-1). The exponentially fed fermentation resulted in yield of 120Uml(-1) alpha-amylase that was thermostable up to 105 degrees C. Results of the exponentially fed fermentation have been discussed in the light of a feed-back controlled fed-batch fermentation reported earlier by the authors. A comparison of the temperature and pH effects on amylase produced by B. caldolyticus and on several other commercially available amylases has also been presented.

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Section: Experiments In Bioreactorssupporting

confidence: 71%