Eisen- und Kobalt-Resorption am perfundierten Dünndarmsegment

Förth, W.

doi:10.1007/978-3-642-95076-6_21

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“…Thus even though the concentration of the iron doses used were similar to those employed by other workers (e.g. Wheby et al 1964;Jacobs et al 1966;Forth, 1968;Huebers et al 1983), the total load of iron presented to the intestinal lumen over the perfusion period was much greater. In addition, in the present study for technical and anatomical reasons only the jejunum and ileum were perfused (since the superior mesenteric artery supplies only the lower half of the duodenum and not the whole duodenum (Greene, 1955)) whereas in most other studies with isolated preparations the duodenum or upper jejunum was used, this being the region of the intestine exhibiting the most rapid rate of iron absorption (Forth & Rummel, 1973).…”

Section: Studies On Iron Absorptionmentioning

confidence: 81%

“…Studies of iron absorption from ligatedloops of rat intestine have indicated that followng instillation of the test dose of59Fe into the lumen, its appearance in either the venous outflow from the portal vein or the carcass is rapid, with linear rates of absorption being observed within 2-10 min of dosing (Wheby, Jones & Crosby, 1964;Forth, 1968).…”

Section: Studies On Iron Absorptionmentioning

confidence: 99%

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Glucose Uptake and Iron Absorption by an Isolated, Vascularly and Luminally Perfused Preparation of Rat Small Intestine

Davies¹,

Coppen

1988

Exp Physiol

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SUMMARYSome characteristics of an isolated vascularly and luminally perfused rat intestinal preparation are described. Over 60 to 90 min perfusion, the vascular perfusion medium (VPM) 'arterial' pressure remained stable and between animals it varied between 22 and 47 mmHg while the VPM flow rate remained constant at 1-3 ml/min. Hypersecretion, as monitored by changes in the concentration of phenol red in the luminal perfusion medium (LPM), did not generally occur. Histological examination of the tissue post-perfusion showed that normal morphology was retained for at least 1 h. Total glucose uptake ranged between 85 and 155 ,umol/(g dry wt. h) with greater uptake from the VPM than the LPM. Approximately 50 % of the glucose taken up by the intestine appeared as lactate in the perfusion media, most of which was released to the VPM. The addition of phloridzin (5 x 10-4 M) to the LPM significantly reduced the uptake of glucose from the LPM and its transfer to the VPM. Iron absorption was studied by adding FeCl2 (with 5"Fe and ascorbate, 100 ,ug/ml) to the LPM to give a final concentration of 10-100 ,ug iron/ml. The appearance of iron in the VPM was investigated over a period of 1 h. At the two lowest concentrations (10 and 25 ,ug/ml) there was a lag of 20-40 min before iron appeared in the VPM. At the higher doses (50 and 100 #tg/ml) the lag was 10 min. During the final 20 min the appearance of iron in the VPM plateaued to dose-dependent constant rates. The ratio of the specific radioactivity of "9Fe in the two perfusates (LPM :VPM) was unity when the initial iron concentration was 25 ,ug/ml or above. At the lowest dose (10 ,ug/ml) this ratio was 2 5 indicating that during transfer, iron taken up from the LPM had mixed with a small labile endogenous iron pool. Both amounts of iron retained by the intestinal tissue and transferred to the VPM increased exponentially with increasing luminal iron dose. The amount of iron retained by the tissue was always greater than that transferred to the VPM.

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