Eisenbestimmung im Serum (Plasma) mit Tripyridyl-triazin (TPTZ) und Citratpuffer. Bewertung der Methode sowie Anpassung an einen SMAC II und RA-1000

Abstract

This method for iron estimation deserves special attention. 1. The specificity for iron is high. The influence ofcopper is approximately 2% only in comparison to methods using ferrozine (up to 15%). Hemoglobin-iron does not disturb the measurement ofplasma or serum iron. 2. The reaction is performed in citrate buffer at a pH from 2.1 to 2.5. This pH is optimal for the liberation of iron from transferrin. At this pH with serum, no precipitation occurs. Thus f a direct photometry is possible without previous protein precipitation. Since this pH is also optimal for colour development the complete reaction can be accomplished in one step and automation ofanalysis can easily be performed. 3. This fest can be performed at Iow cost. Compared to commercial tests, the price can be reduced between V A andV, ö .

Automated Determination of Serum (Plasma) and Urine Iron: A Comparative Investigation of Chromogens. Improved Tripyridyltriazine Micromethod

“…The colour complexes of tripyridyl-s-triazine and ference-S were found to be stable for at least 72 h, and for bathophenanthroline sulphonate and ferrozine for at least 36 and 24 h, respectively. The interference of copper in the tripyridyls-triazine/citrate method was found to be less than 2.0% in a plasma with various concentrations of added copper chloride, as described in detail elsewhere (13).…”

“…Adaptation to other automated systems The tripyridyl-s-triazine/citrate method was adapted to a Technicon SMAC II and Technicon RA 1000 (13), Eppendorf Epos and Abbot Spectrum automated analysers. The method can be run at any commonly used temperature.…”