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Abstract

The plant Scoparia dulcis Linn. belongs to the family Scrophulariaceae, is a native of tropical America, commonly called a sweet broom. The traditional healers identified its uses as anti-diabetic, antipyretic, antihypertensive, diuretic, and they have developed many promising traditional medicines and formulations from this plant. The main objective of this present study reveals about the phytoconstituents present in the leaves extract by preliminary phytochemical study, isolation of active compounds by column chromatography, and characterize the isolated compound by spectral studies like UV, IR, NMR, and MASS spectra. The preliminary phytochemical study reveals that the plant possesses more phytochemicals such as carbohydrates, alkaloids, tannins, phenols, flavonoids, vitamin C, and amino acids. The quantitative estimation shows that it consist of a considerable quantity of flavonoids, Phenols, Vitamin C. The methanol extract was prepared and applied in column chromatography to isolate the phytoconstituents. A dull white crystalline compound was isolated in the fraction of Acetone: Ethanol (60:40), (40:60) ratio. This compound was subjected to spectral studies. The spectral studies indicate the presence of -OH, C=O, and C-H groups from the NMR signal. It shows the presence of methyl proton and α -CH 2 proton. Based on the spectral studies, the chemical nature of the isolated compound may be a heptadeconic acid with the molecular formula CH 3 -(CH 2 ) 15 -COOH. Future studies also can go for further identification of new compounds and their medicinal values. INTRODUCTION: Phytochemicals are the complex mixture of chemicals naturally synthesized by plants, and they are a very important base for phytotherapy and nutritional sciences. Some are completely characterized, and some are incompletely characterized and have recently been subject to scientific scrutiny 1 . Abundant of photochemical are present in plant sources without knowing their chemical nature and therapeutic values.