Eksplorasi Bakteri Antagonis Asal Jaringan Dan Rizosfer Tanaman Karet Untuk Menekan Pertumbuhan Bakteri Proteolitik Pada Bahan Olahan Karet (Bokar)

Abstract

Latex is a mainstay of agriculture commodity and one of the most important sources of Indonesia's foreign exchange earnings. However, the latex storage spreads odor to the air. The goal of this research is to explore antagonistic bacteria from rubber plant tissue and rhizosfer. These antagonistic bacteria can suppress the growth of proteolitic microbe that caused odor in rubber slab. The proteolitic bacteria are isolated from various shelf life of rubber slab. Antagonistic bacteria are isolated from rubber plant tissue and rhizosfer. The ability of antagonistic bacteria to suppress the growth of proteolitic bacteria is done through inhibition test in agar nutrient medium (NA). The diameter of clear zone formed is the indication of antagonistic bacteria ability in suppressing proteolitic bacteria activity. From this research, found 2 bacteria isolate that has the potency to be developed as antagonistic bacteria to suppress proteolitic bacteria activity in the storage process. Both of these antagonistic bacteria are isolates D 1 U 1 that comes from the leaves of rubber plant, and isolate A 2 U 2 that comes from the root of rubber plant. These isolates produce the widest average of inhibition zone with diameter of 11.37 mm and 11.29 mm.

Study of combination of liquid organic waste on antifungal activity Bacillus sp. DB12

“…A wider diameter of the clear zone indicates that the antifungal compound has good activity [26]. According to Gofar et al ( 2019) the presence of a clear zone is an indication of the ability of antagonistic bacteria to inhibit the activity of pathogenic fungi [27]. Prihatiningsih and Djatmiko (2016) reported that the formation of a clear zone indicated that the antagonistic bacteria produced secondary metabolic compounds in the form of antibiotics, toxins or enzymes [28].…”

Keragaman dan Resistensi Antibiotik Isolat Bakteri Tanah di Dalam dan Luar TPU Bonoloyo, Surakarta Jawa Tengah

“…Hasil penelitian (Putra et al, 2023), mendapatkan total bakteri tanah rizosfer di area TPU sebanyak 1,0 x 10 7 CFU/g. Jumlah ini lebih banyak dibanding total bakteri rizosfer di luar area TPU, misalnya dari rizosfer tanaman kemiri sunan sebanyak 7,6 x 10 5 CFU/g (Amaria et al, 2019), rizosfer tanaman karet sebanyak 2,7 x 10 4 CFU/g (Gofar et al, 2014), dan rizosfer tanaman caisim sebanyak 4,3 x 10 4 CFU/g (Hindersah et al, 2018). Kemelimpahan bakteri rizosfer di TPU tersebut menunjukkan bahwa dekomposisi jenazah berpengaruh terhadap mineral tanah yang diperlukan untuk pertumbuhan bakteri (Utomo et al, 2018 (Całkosiński et al, 2015).…”