Ekspresi Relatif mRNA BRLF1 Epstein-Barr Virus dari Biopsi Jaringan Tumor dalam Blok Parafin sebagai Petanda Biologi Patogenesis Karsinoma Nasofaring

Abstract

Nasopharyngeal carcinoma (NPC) is a malignant tumor of epithelial cells of the nasopharynx and is a multifactorial disease that is geographically endemic in the world. The undifferentiated carcinoma is the most common type of NPC and strongly associated with Epstein-Barr virus (EBV) infection. BRLF1 lytic gene is required for the switch from latent to lytic infection. The pathogenesis of NPC is more directly reflected by carcinoma-specific viral transcriptional activity at the site of primary tumor. The aim of this study was to determine the relative expression of mRNA BRLF1 EBV in NPC WHO-3 patient as a potential biomarker of NPC pathogenesis, particularly in tumor progressivity in NPC. This research designed by an analytical observasional research with a cross sectional study. The samples were the tissue biopsies formalin-fixed embedded paraffin (FFPE) of 24 NPC patients at Pathology Anatomy Departement, RSUD. Prof. dr. Margono Soekarjo, Purwokerto. The relative expression of mRNA BRLF1 EBV was determined by RT-qPCR technique and calculated by Livak & Schmittgen formula (2 -ΔCt ). The relative expression of mRNA BRLF1 EBV in NPC WHO-3 patients on advanced staging was approximately start from 99.04415959 to 1097.496026. In NPC WHO-3 patients, the relative expression of mRNA BRLF1 EBV in early stage (n = 5; 544,72420 + 142,614733) increased of 1.8 times compared to late stage (n = 17; 395,68612 + 293,172201), however it was no significance differences statistically (n = 17; df =1; p=0,13). Therefore, the relative expression of mRNA BRLF1 EBV from tissue biopsies FFPE are not potensial biomarker of NPC pathogenesis, particularly in tumor progressivity on advanced staging. Key Words : Biomarker pathogenesis, NPC, Relative expression of mRNA BRLF1 EBV BRLF1 AbstrakKarsinoma nasofaring (KNF) merupakan tumor ganas pada sel epitel nasofaring dan merupakan penyakit multifaktor yang bersifat endemik. Tipe KNF tidak berdiferensiasi (KNF WHO-3) telah terbukti konsisten dengan infeksi EBV. Gen litik BRLF1 berperan sebagai mediator transisi dari fase laten menjadi litik. Pengukuran aktivitas mRNA EBV di lokasi tumor primer perlu dilakukan karena lebih mencerminkan patogenesis KNF yang sesungguhnya dari pada diagnosis serologi dan pengukuran DNA EBV di sirkulasi darah. Penelitian ini bertujuan untuk mengetahui nilai ekspresi relatif mRNA BRLF1 Epstein-Barr Virus pada beberapa tingkat stadium tumor penderita KNF sebagai petanda biologi potensial dalam patogenesis KNF. Metode penelitian yang digunakan adalah metode deskriptif yang berbentuk studi seran lintang. Sampel penelitian ini adalah biopsi jaringan tumor dalam blok parafin penderita KNF sebanyak 24 sampel. Sampel tersebut telah didiagnosis pasti sebagai KNF WHO-3 dari hasil pemeriksaan Patologi Anatomi pada Poli Patologi Anatomi, RSUD Prof. Dr. Margono Soekarjo, Purwokerto serta memenuhi kriteria inklusi sampel. Analisis statistik dilakukan untuk membandingkan ekspresi relatif mRNA BRLF1 stadium yang berbeda. Nilai ekspresi relatif mRNA BRLF1 EBV pada 24 sampel ...

Family Support: A Caregiver Experience in Caring for Nasopharynx Cancer Patients in Yogyakarta

“…Nasopharyngeal cancer is a carcinoma that occurs in the nasopharynx epithelial cells, which are located above the throat and behind the nose. Nasopharyngeal cancer is also a multifactorial disease that is endemic [1]. Risk factors in patients with nasopharyngeal cancer are associated with non-viral exposure and have a strong association with salted fish.…”