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Higuchi, Akon; Yoshida, Makoto; Ohno, Takeshi; Asakura, Tetsuo; Hara, Mariko

doi:10.1023/a:1008130223190

Cited by 23 publications

(10 citation statements)

References 18 publications

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“…casting and LB methods, are a strong factor affecting cell growth. The concentration and production of interferon-¯per unit number of cells on the LB membranes were found to be higher than those on the cast membranes made of the same proteins except collagen [21].…”

Section: Introductionmentioning

confidence: 77%

“…collagen, bronectin, laminin, and vitronectin) [21,22]. The cell density of the broblast cells cultured on the LB membranes was found to be higher than that on the cast membranes made of bronectin, vitronectin, and collagen-blended membranes.…”

Section: Introductionmentioning

confidence: 92%

“…In our previous studies [19][20][21][22], cell growth and production of interferon-¯were investigated for broblasts (NB1-RGD) cultured on Langmuir-Blodgett (LB) and cast membranes prepared from the extracellular matrix proteins (e.g. collagen, bronectin, laminin, and vitronectin) [21,22].…”

Section: Introductionmentioning

confidence: 99%

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Enhanced production of carcinoembryonic antigen by CW-2 cells cultured on polymeric membranes immobilized with extracellular matrix proteins

Hara¹,

Adachi²,

Higuchi³

2003

Journal of Biomaterials Science, Polymer Edition

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Cell growth and the production of carcinoembryonic antigen (CEA) were investigated in human colorectal adenocarcinoma tumor (CW-2) cells cultured on extracellular matrix (ECM) protein membranes, heat-treated poly(vinyl alcohol-co-ethylamine) (PVA-EA) membranes, and PVA-EA membranes containing immobilized ECM proteins. The highest concentration of CEA was found in the cell culture media of CW-2 cells on collagen (COL)-immobilized PVA-EA membranes. This is explained by the flexible mobility of COL on the COL-immobilized PVA-EA membranes causing a specific cell response for the production of CEA. An inverse relationship was observed between either the cell density or the CEA concentration in the cell culture media and the amount of fibronectin (FN) adsorbed on the COL-immobilized membranes. The CEA concentration in the cell culture media was directly related to the cell density, which, in turn, is inversely related to the amount of FN secreted by CW-2 cells. These findings indicate that cells tend to attach to the surface by secreting ECM proteins such as FN when they are grown on substrates that provide weak cell attachment.

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Section: Introductionmentioning

confidence: 77%

Section: Introductionmentioning

confidence: 92%

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Enhanced production of carcinoembryonic antigen by CW-2 cells cultured on polymeric membranes immobilized with extracellular matrix proteins

Hara¹,

Adachi²,

Higuchi³

2003

Journal of Biomaterials Science, Polymer Edition

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“…Silk fibroin is a natural polymer, produced by silk worms, that can be mass produced, and has been used as a textile fiber. Recently, many studies have reported silk use in nontextile fields, especially the biomedical field, as a substrate for enzyme immobilization,1, 2 bone‐compatible materials,3, 4 antithrombogenic material,5, 6 wound dressing,7, 8 drug carrier,9 and as a cell culture substrate 10–13. These findings indicate that silk fibroin will be a good basic component for new biomedical material development.…”

Section: Introductionmentioning

confidence: 99%

Sulfation of silk fibroin by sulfuric acid and anticoagulant activity

Tamada

2003

J of Applied Polymer Sci

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Silk fibroin (Bombyx mori) was sulfated with an aqueous sulfuric acid solution. The sulfated fibroin was characterized by Fourier transform infrared spectroscopy (FTIR), amino acid analysis, and gel filtration chromatography (GFC). Maximum yield was obtained at around 2-4 h, and it decreased at 6 h and more. The molecular size decreased and dispersed with sulfation, and the molecular weight was estimated at around 10,000 by GFC using protein standards. The amino acid composition indicated that the crystal region of the fibroin molecule remained in sulfated fibroin until a sulfation reaction time of 4 h. The incorporation of sulfate groups was confirmed by FTIR and the amount of sulfate groups introduced for 4 h sulfation was estimated in 0.3 mmol/g by acidimetric titration. The efficiency of sulfation was calculated at 15.7%. Blood coagulation was prevented by 20 mg of sulfated fibroin in 1 mL of blood, while original fibroin did not show the effect. This result indicates that sulfate group introduction results in addition of anticoagulant function to silk fibroin. Although a variety of polymer backbones have been used for synthesis of sulfated polymers as anticoagulant materials, no reports are available concerning a sulfated polymer based on a protein backbone. Sulfated fibroin is a new type of anticoagulant material having a protein backbone.

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“…15 There have been very few prior studies of pure collagen or collagen/phospholipid LB films at vapor/liquid interfaces or on a solid support. 16,17 At 25 1C, a 0.1 wt% acetic solution of type I collagen (pH 3.0) was spread onto the surface of ultrapure water in a LB trough and successfully transferred onto silicon-coated glass by horizontal deposition. 16 Ghannam et al used a different method to incorporate collagen at the air/water interface by injecting a collagen solution in 0.5M acetic acid into the aqueous subphase with or without a phospholipid spread monolayer on the surface.…”

Section: Introductionmentioning

confidence: 99%

Biocomposite films synthesized at a fluid/fluid interface

Rathman¹,

Sun²

2005

Faraday Disc.

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In the synthesis of mesostructured particles and films, the cooperative self-organization of amphiphilic molecules in the presence of reactive species is a key factor in the reaction mechanism. This paper presents a method for preparing structured collagen films synthesized at fluid/fluid interfaces. This work is an extension of previous efforts in our group to synthesize structured silica films in a reaction system confined at the interface between two immiscible fluid phases, providing an additional level of control over the structural evolution that occurs during reaction. Synthesis at a liquid/liquid interface was shown to provide excellent control over the mesostructure of the final product, avoiding a major problem encountered in many film synthesis techniques in which the reaction occurs at a liquid/solid interface: namely, the undesired effect of the solid surface on the film structure. The focus of this paper is the synthesis of structured composite films containing amphiphilic phospholipids and collagen. These films provide a way to pattern cell growth on biocompatible surfaces and a model system for studying the self-assembly mechanism of lipids and collagen. Self-assembled monolayers and bilayers composed of phosphatidylethanolamine (PE) lipids and collagen were investigated to determine how regularly patterned films can be prepared in a manner that preserves the bioactive properties of the collagen. Mixtures of PEs and acid-soluble collagen were spread on an aqueous subphase in a Langmuir trough. Surface pressure-area compression isotherms for the composite lipid/collagen monolayers provide information about interactions between these components. Langmuir-Blodgett (LB) techniques were utilized to transfer the composite films onto freshly cleaved mica. The mica-supported films were characterized by atomic force microscopy. The lipid/collagen ratio in the composite films was found to be the most important factor in determining how the collagen is assembled and distributed. The temperature and pH of the aqueous subphase, the process for spreading collagen on the subphase, the deposition speed, and the deposition pressure are factors that can be used to selectively control the film patterning. For most of these experimental factors, the range over which a highly structured uniform film can be fabricated over significant length scales is generally very narrow. Based on the experimental results and understanding of the fundamental interactions involved, a mechanism for the co-self-assembly of phospholipids and collagen is suggested. The adhesion and growth of Chinese hamster ovary (CHO) cells on the patterned film surfaces demonstrates the biocompatibility of these composite films.

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Cited by 23 publications

References 18 publications

Enhanced production of carcinoembryonic antigen by CW-2 cells cultured on polymeric membranes immobilized with extracellular matrix proteins

Enhanced production of carcinoembryonic antigen by CW-2 cells cultured on polymeric membranes immobilized with extracellular matrix proteins

Sulfation of silk fibroin by sulfuric acid and anticoagulant activity

Biocomposite films synthesized at a fluid/fluid interface

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