Glucose detection is considered a significant area and has remained the topic of considerable attention. Remarkable technological advancements have been observed in diabetes monitoring in the past decades. This continual progress helps to track recent trends in development as well as identify challenging issues in glucose sensor construction. Thus, a comprehensive synopsis of the most recent advancements and developments in the study of nickel (Ni) nanostructure-based sensors for efficient trace-level glucose detection, following non-enzymatic and electrochemical methods, is provided in this review. Moreover, this review is intensively focused on the methodologies for the structure, morphology, preparation, and enforcement of a variety of Ni nanostructures, including Ni nanosheets with metals, Ni nanospheres with metals/mixed metals, Ni-metal nanocomposites, metal nanoparticles-decorated Ni nanowires, Ni nanoparticles, Ni-decorated metal nanotube arrays, Ni nanoneedles and nanorods with metals, nanoporous, nanoplates, nanocoated Ni with metal composites, and Ni-composed hybrid nanostructures. Various demonstrations and categorizations are provided on Ni-based nanostructures for a clear understanding for diverse readers.