Elaboration of antimicrobial polymeric materials by dispersion of well-defined amphiphilic methacrylic SG1-based copolymers

Benkhaled, Belkacem Tarek; Hadiouch, Slim; Olleik, Hamza; Perrier, Josette; Ysacco, Cédric; Guillaneuf, Yohann; Gigmès, Didier; Maresca, Marc; Lefay, Catherine

doi:10.1039/c8py00523k

Cited by 33 publications

(41 citation statements)

References 54 publications

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“…Cells were routinely grown on 25 cm 2 flasks in Dulbecco's modified essential medium (DMEM) supplemented with 10% fetal calf serum (FCS), 1% l-glutamine, and 1% antibiotics (Invitrogen©, Carlsbad, CA, USA), and maintained in a 5% CO 2 incubator at 37 • C. For innocuity testing, CHO cells grown on flasks were trypsinized, diluted in culture media, and seeded onto 96-well plates (Greiner Bio-One©, Austria) at 20,000 cells per well [16]. After seeding, cells were left to reach confluence for 48 h. Confluent cells were then exposed to increasing doses of compounds 1-7 or ascorbic acid for 24 h at 37 • C before evaluation of the cell viability using a resazurin-based in vitro toxicity assay kit (Sigma-Aldrich), as previously described [17,18].…”

Section: Cell Innocuity and Protective Effect Against H 2 O 2 -Mediatmentioning

confidence: 99%

Naphtho-Gamma-Pyrones Produced by Aspergillus tubingensis G131: New Source of Natural Nontoxic Antioxidants

Carboué¹,

Maresca

Herbette

et al. 2019

Biomolecules

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Seven naphtho-gamma-pyrones (NγPs), including asperpyrone E, aurasperone A, dianhydroaurasperone C, fonsecin, fonsecinone A, fonsecin B, and ustilaginoidin A, were isolated from Aspergillus tubingensis G131, a non-toxigenic strain. The radical scavenging activity of these NγPs was evaluated using ABTS assay. The Trolox equivalent antioxidant capacity on the seven isolated NγPs ranged from 2.4 to 14.6 μmol L−1. The toxicity and ability of the NγPs to prevent H2O2-mediated cell death were evaluated using normal/not cancerous cells (CHO cells). This cell-based assay showed that NγPs: (1) Are not toxic or weakly toxic towards cells and (2) are able to protect cells from oxidant injuries with an IC50 on H2O2-mediated cell death ranging from 2.25 to 1800 μmol mL−1. Our data show that A. tubingensis G131 strain is able to produce various NγPs possessing strong antioxidant activities and low toxicities, making this strain a good candidate for antioxidant applications in food and cosmetic industries.

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Section: Cell Innocuity and Protective Effect Against H 2 O 2 -Mediatmentioning

confidence: 99%

Naphtho-Gamma-Pyrones Produced by Aspergillus tubingensis G131: New Source of Natural Nontoxic Antioxidants

Carboué¹,

Maresca

Herbette

et al. 2019

Biomolecules

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“…Bacterial resistance is considered as a major public health concern, particularly when considering hospitals where nosocomial infections are prevalent in intensive care units [1,2]. The World Health Organization proposed that “we are heading towards a post-antibiotic era in which common infections and minor injuries can once again kill”.…”

Section: Introductionmentioning

confidence: 99%

Comparative Structure–Activity Analysis of the Antimicrobial Activity, Cytotoxicity, and Mechanism of Action of the Fungal Cyclohexadepsipeptides Enniatins and Beauvericin

Olleik

Nicoletti

Lafond

et al. 2019

Toxins

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Filamentous fungi, although producing noxious molecules such as mycotoxins, have been used to produce numerous drugs active against human diseases such as paclitaxel, statins, and penicillin, saving millions of human lives. Cyclodepsipeptides are fungal molecules with potentially adverse and positive effects. Although these peptides are not novel, comparative studies of their antimicrobial activity, toxicity, and mechanism of action are still to be identified. In this study, the fungal cyclohexadepsipeptides enniatin (ENN) and beauvericin (BEA) were assessed to determine their antimicrobial activity and cytotoxicity against human cells. Results showed that these peptides were active against Gram-positive bacteria, Mycobacterium, and fungi, but not against Gram-negative bacteria. ENN and BEA had a limited hemolytic effect, yet were found to be toxic at low doses to nucleated human cells. Both peptides also interacted with bacterial lipids, causing low to no membrane permeabilization, but induced membrane depolarization and inhibition of macromolecules synthesis. The structure–activity analysis showed that the chemical nature of the side chains present on ENN and BEA (either iso-propyl, sec-butyl, or phenylmethyl) impacts their interaction with lipids, antimicrobial action, and toxicity.

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“…Mycobacterium smegmatis (mc2155, ATCC 700084) was used as model of Mycobacterium species. Bacteria were cultured as previously described [ 15 , 16 , 17 , 18 ] Briefly, most of the bacterial strains were routinely grown on Luria Bertani (LB) agar plates and LB broth at 37 °C in aerobic condition except H. pylori that was grown in BHI in micro-aerobic condition using microaerobic BD GasPak generator (Sigma-Aldrich, Lyon, France). M. smegmatis was cultured in Middlebrook 7H10 agar plate and Middlebrook 7H10 broth at 37 °C in aerobic condition.…”

Section: Methodsmentioning

confidence: 99%

“…Antimicrobial activity of berberine and its derivatives was evaluated by determination of their minimal inhibitory concentration (MIC) using two-fold serial dilutions in bacterial liquid media following the National Committee of Clinical Laboratory Standards (NCCLS, 1997) as previously described [ 15 , 16 , 17 , 18 ]. Briefly, for most of the bacteria, single colonies of the different bacterial strains cultured on specific agar plates were used to inoculate 3 mL of Mueller-Hinton (MH), except C. difficile , C. perfringens , H. pylori , E. faecalis , and S. pyogenes that were cultured in 3 mL of BHI and or M. smegmatis that was cultured in 3 mL of Middlebrook 7H10 broth.…”

Section: Methodsmentioning

confidence: 99%

Synthesis and Evaluation of the Antibacterial Activities of 13-Substituted Berberine Derivatives

et al. 2020

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The biological activities of berberine, a natural plant molecule, are known to be affected by structural modifications, mostly at position 9 and/or 13. A series of new 13-substituted berberine derivatives were synthesized and evaluated in term of antimicrobial activity using various microorganisms associated to human diseases. Contrarily to the original molecule berberine, several derivatives were found strongly active in microbial sensitivity tests against Mycobacterium, Candida albicans and Gram-positive bacteria, including naïve or resistant Bacillus cereus, Staphylococcus aureus and Streptococcus pyogenes with minimal inhibitory concentration (MIC) of 3.12 to 6.25 µM. Among the various Gram-negative strains tested, berberine’s derivatives were only found active on Helicobacter pylori and Vibrio alginolyticus (MIC values of 1.5–3.12 µM). Cytotoxicity assays performed on human cells showed that the antimicrobial berberine derivatives caused low toxicity resulting in good therapeutic index values. In addition, a mechanistic approach demonstrated that, contrarily to already known berberine derivatives causing either membrane permeabilization, DNA fragmentation or interacting with FtsZ protein, active derivatives described in this study act through inhibition of the synthesis of peptidoglycan or RNA. Overall, this study shows that these new berberine derivatives can be considered as potent and safe anti-bacterial agents active on human pathogenic microorganisms, including ones resistant to conventional antibiotics.

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Elaboration of antimicrobial polymeric materials by dispersion of well-defined amphiphilic methacrylic SG1-based copolymers

Abstract: Towards a versatile and easy method of elaboration of solid polymeric antimicrobial materials.

Cited by 33 publications

References 54 publications

Naphtho-Gamma-Pyrones Produced by Aspergillus tubingensis G131: New Source of Natural Nontoxic Antioxidants

Naphtho-Gamma-Pyrones Produced by Aspergillus tubingensis G131: New Source of Natural Nontoxic Antioxidants

Comparative Structure–Activity Analysis of the Antimicrobial Activity, Cytotoxicity, and Mechanism of Action of the Fungal Cyclohexadepsipeptides Enniatins and Beauvericin

Synthesis and Evaluation of the Antibacterial Activities of 13-Substituted Berberine Derivatives

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