Elastase, α<sub>1</sub>‐proteinase inhibitor, and interleukin‐8 in pre‐dialyzed and hemodialyzed patients with chronic kidney disease

Polańska, Bożena; Augustyniak, Daria; Makulska, Irena; Niemczuk, Maria; Zwołińska, Danuta; Jankowski, A

doi:10.1111/j.1442-200x.2010.03162.x

Cited by 10 publications

(16 citation statements)

References 40 publications

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“…In CKD, plasma elastase levels are elevated, [39][40][41] and primed neutrophils are associated with inflammation and oxidative stress. 28 Our findings indicate that regular exercise does not exacerbate the activation state of resting neutrophils (although transient spontaneous neutrophil activation occurs during each exercise bout).…”

Section: Effects Of Regular Exercisementioning

confidence: 99%

Evidence for Anti-Inflammatory Effects of Exercise in CKD

Viana

Kosmadakis

Watson

et al. 2014

Journal of the American Society of Nephrology

160

128

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CKD is associated with a complex state of immune dysfunction characterized by immune depression, predisposing patients to infections, and immune activation, resulting in inflammation that associates with higher risk of cardiovascular disease. Physical exercise may enhance immune function and exert antiinflammatory effects, but such effects are unclear in CKD. We investigated the separate effects of acute and regular moderate-intensity aerobic exercise on neutrophil degranulation (elastase release), activation of T lymphocytes (CD69 expression) and monocytes (CD86 and HLA-DR expression), and plasma inflammatory markers (IL-6, IL-10, soluble TNF-receptors, and C-reactive protein) in patients with predialysis CKD. A single 30-minute (acute) bout of walking induced a normal pattern of leukocyte mobilization and had no effect on T-lymphocyte and monocyte activation but improved neutrophil responsiveness to a bacterial challenge in the postexercise period. Furthermore, acute exercise induced a systemic anti-inflammatory environment, evidenced by a marked increase in plasma IL-10 levels (peaked at 1 hour postexercise), that was most likely mediated by increased plasma IL-6 levels (peaked immediately postexercise). Six months of regular walking exercise (30 min/d for 5 times/wk) exerted anti-inflammatory effects (reduction in the ratio of plasma IL-6 to IL-10 levels) and a downregulation of T-lymphocyte and monocyte activation, but it had no effect on circulating immune cell numbers or neutrophil degranulation responses. Renal function, proteinuria, and BP were also unaffected. These findings provide compelling evidence that walking exercise is safe with regard to immune and inflammatory responses and has the potential to be an effective anti-inflammatory therapy in predialysis CKD.

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Section: Effects Of Regular Exercisementioning

confidence: 99%

Evidence for Anti-Inflammatory Effects of Exercise in CKD

Viana

Kosmadakis

Watson

et al. 2014

Journal of the American Society of Nephrology

160

128

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“…There were no significant differences between B-CAPD and CT patients in reference to all three measured indicators (Table 2). The differences between CT patients and controls have been published previously (Polańska et al 2010). …”

Section: Resultsmentioning

confidence: 72%

“…The patients with autoimmune disease had complete clinical and biochemical remission. Additionally, for comparative purposes the previously published conservatively treated (CT) group was included (Polańska et al 2010). The CT group consisted of 13 patients, 6 females (46 %) and 7 males (54 %), aged 4–17 years (mean 12 ± 4.5 years), The causes of CKD in the CT group were: chronic glomerular nephritis ( n = 4), hydronephrosis ( n = 1), polycystic kidney disease ( n = 4), kidney hypoplasia ( n = 1), chronic interstitial nephritis ( n = 1), and posterior urethral valves ( n = 2).…”

Section: Methodsmentioning

confidence: 99%

“…One of the major and natural inhibitors of elastase is α 1 PI (Korkmaz et al 2005a), which protects the surrounding tissues from enzyme-mediated destruction. Neutrophil elastase, both in the free form and in enzymatically, inactive complex with α 1 PI, may be a good indicator of the neutrophil activity (Polańska et al 2004) as well as the degree of inflammatory reactions in acute and chronic diseases, including kidney disorders requiring dialysis (Donovan et al 1993; Polańska et al 2006, 2010; Shutov et al 1999). IL-8 is the major chemotactic factor for neutrophils which also participates in their activation (Baggiolini and Clark-Lewis 1992) and degranulation (Segura et al 1998).…”

Section: Introductionmentioning

confidence: 99%

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Elastase, α1-Proteinase Inhibitor, and Interleukin-8 in Children and Young Adults with End-Stage Kidney Disease Undergoing Continuous Ambulatory Peritoneal Dialysis

Polańska

Augustyniak

Makulska

et al. 2013

Arch. Immunol. Ther. Exp.

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Peritoneal dialysis is one of the main modality of treatment in end-stage kidney diseases (ESKD) in children. In our previous work in chronic kidney disease patients, in pre-dialyzed period and on hemodialysis, the neutrophils were highly activated. The aim of this study was to assess an inflammatory condition and neutrophil activation in ESKD patients undergoing continuous ambulatory peritoneal dialysis (CAPD). Thirteen CAPD patients without infection, both sexes, aged 2.5–24 years, and group of healthy subjects (C) were studied. For comparative purposes the conservatively treated (CT) group of ESKD patients was included. Neutrophil elastase in complex with α1-proteinase inhibitor (NE-α1PI; ELISA), α1-proteinase inhibitor (α1PI; radial immunodiffusion) and interleukin-8 (IL-8; ELISA) were measured in the blood samples from CAPD, CT, and C group and in the peritoneal dialysate fluid (PDF) samples of patients on CAPD. A significantly increased plasma NE-α1PI levels (median 176.5 μg/L, range 85.2–373.2 μg/L; p < 0.00005), serum IL-8 (median 18.6 pg/mL, range 15.73–35.28 pg/mL; p < 0.05), and slightly decreased serum α1PI (median 1,540 mg/L, range 1,270–1,955; p ≤ 0.05) compared to the control groups were found. There were no significant differences of analyzed parameters between CAPD and CT patients. The concentration ratio of NE-α1PI, α1PI and IL-8 in blood/PDF was 29.97, 8.24, and 4.48, respectively. There were significantly positive correlations between serum and PDF concentration of α1PI and IL-8 (r = 0.613, p < 0.05; r = 0.59; p < 0.005, respectively). The results of our study demonstrate that neutrophils are highly activated in non-infected CAPD patients. The pivotal marker of this activation is NE-α1PI. It may contribute to chronic inflammation and tissues injury.

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“…The ability of NE to inactivate the complement components C3 and C5a, immunoglobulin, proteinase inhibitors, clotting factors and adhesion molecules have a direct impact on the course of inflammatory reactions [9]. Secondly, the concentration of neutrophil elastase forming a complex with α 1 -proteinase inhibitor (NE-α 1 PI) correlates with the released free NE elastase and can be used as a measure for the activity of neutrophils during an inflammatory response in different diseases [15][16][17][18].…”

Section: Introductionmentioning

confidence: 99%

Clinical immunology Assessment of neutrophil elastase plasma levels in children with intermediate uveitis

Polańska¹,

Turno-Kręcicka²,

Kaźmierowska-Niemczuk³

2012

cejoi

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Elastase, α₁‐proteinase inhibitor, and interleukin‐8 in pre‐dialyzed and hemodialyzed patients with chronic kidney disease

Cited by 10 publications

References 40 publications

Evidence for Anti-Inflammatory Effects of Exercise in CKD

Evidence for Anti-Inflammatory Effects of Exercise in CKD

Elastase, α1-Proteinase Inhibitor, and Interleukin-8 in Children and Young Adults with End-Stage Kidney Disease Undergoing Continuous Ambulatory Peritoneal Dialysis

Clinical immunology Assessment of neutrophil elastase plasma levels in children with intermediate uveitis

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Elastase, α1‐proteinase inhibitor, and interleukin‐8 in pre‐dialyzed and hemodialyzed patients with chronic kidney disease

Cited by 10 publications

References 40 publications

Evidence for Anti-Inflammatory Effects of Exercise in CKD

Evidence for Anti-Inflammatory Effects of Exercise in CKD

Elastase, α1-Proteinase Inhibitor, and Interleukin-8 in Children and Young Adults with End-Stage Kidney Disease Undergoing Continuous Ambulatory Peritoneal Dialysis

Clinical immunology Assessment of neutrophil elastase plasma levels in children with intermediate uveitis

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Product

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Elastase, α₁‐proteinase inhibitor, and interleukin‐8 in pre‐dialyzed and hemodialyzed patients with chronic kidney disease