Elastic property of sickle cell anemia and sickle cell trait red blood cells

Abstract

. Significance: We introduce a model for better calibration of the trapping force using an equal but oppositely directed drag force acting on a trapped red blood cell (RBC). We demonstrate this approach by studying RBCs’ elastic properties from deidentified sickle cell anemia (SCA) and sickle cell trait (SCT) blood samples. Aim: A laser trapping (LT) force was formulated and analytically calculated in a cylindrical model. Using this trapping force relative percent difference, the maximum (longitudinal) and minimum (transverse) radius rate and stiffness were used to study the elasticity. Approach: The elastic property of SCA and SCT RBCs was analyzed using LT technique with computer controlled piezo-driven stage, in order to trap and stretch the RBCs. Results: For all parameters, the results show that the SCT RBC samples have higher elastic property than the SCA RBCs. The higher rigidity in the SCA cell may be due to the lipid composition of the membrane, which was affected by the cholesterol concentration. Conclusions: By developing a theoretical model for different trapping forces, we have also studied the elasticity of RBCs in SCT (with hemoglobin type HbAS) and in SCA (with hemoglobin type HbSS). The results for the quantities describing the elasticity of the cells consistently showed that the RBCs in the SCT display lower rigidity and higher deformability than the RBCs with SCA.

The radiation response measurement of a single and multiple cell ionization of neuroblastoma cells by infrared laser trap

“…Let us recall that in RT, the therapeutic ratio is the maximum radiation dose by which the death of cancer cells is locally controlled and the minimum radiation dose by which cells in normal tissues have low acute and late morbidity [ 35 ]. Therefore, since TRD is decreased with the increased mass of N2a and 4T1 cells, we believe that the chain ionization mechanism [ 20 ] brings about this reduction in TRD ( Fig. 9(b) ).…”

“…Therefore, in this study, we use high-power LT to better understand the therapeutic efficacies of carcinoma cells. LT is an advanced technique that utilizes radiation force to capture, translate and manipulate microscopic particles [ 10 , 20 ]. This new experimental method has played a revolutionary role in the areas of physical and biological sciences.…”

“…This new experimental method has played a revolutionary role in the areas of physical and biological sciences. It was recently demonstrated that the ionization energy (IE) and the charge developed could be determined at the single-cell level for BT20 breast cancer [ 19 ] and RBCs cells [ 18 , 20 , 21 ]. Further, in a high-power trap (50 mW), the blood cells undergo hemolysis as they become charged, and are ejected, which is not the case in a low-power trap.…”

Respon Fisiologis dan Hematologi Kelinci Rex yang diberi Pakan Mengandung Indigofera zollingeriana

“…Eritrosit kurang dari normal menyebabkan terjadinya anemia sedangkan eritrosit meningkat menyebabkan terjadinya polisitemia (Muhammed et al, 2021). Menurunnya eritrosit pada kelinci menyebabkan rendahnya pertumbuhan pada kelinci.…”

The Effectiveness of Suffruticosol B. in Treating Lung Cancer by Laser Trapping Technique

“…LT has the potential to help determine a better therapeutic ratio based on the cellular level, to eradicate radiation-induced tissue toxicity while advancing the sterilization of the cancerous cells 7,8 [7,8]. This technique provides unique means to control the dynamics of small particles and manipulate biological samples at the micron level 17,18 [17,18]. In the physical and biological sciences, this experiment has played a fundamental role.…”