Elasticity-based identification of tumor margins using Brillouin spectroscopy

Troyanova‐Wood, Maria; Meng, Zhaokai; Yakovlev, Vladislav V.

doi:10.1117/12.2213268

Cited by 7 publications

(7 citation statements)

References 29 publications

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“…The high-frequency longitudinal elastic modulus can, therefore, be calculated directly from the Brillouin shift for backscattered signal collection as

M^{'} = \frac{1}{4} ρ {(\frac{normalΔ ν_{B} λ_{0}}{n})}^{2}

. Brillouin elastography (also known as Brillouin spectroscopy), an emerging spectroscopic technique, [11,12] is capable of assessing the elasticity for biomedical applications on the level of tissue [13–15] and individual cells. [16,17]…”

Section: Introductionmentioning

confidence: 99%

Optical assessment of changes in mechanical and chemical properties of adipose tissue in diet‐induced obese rats

Troyanova‐Wood

Gobbell

Meng

et al. 2017

Journal of Biophotonics

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Obesity is becoming a leading cause of health problems world-wide. Obesity and overweight are associated with the structural and chemical changes in tissues; however, few methods exist that allow for concurrent measurement of these changes. Using Brillouin and Raman microspectroscopy, both the mechanical and chemical differences can be assessed simultaneously. We hypothesized that Brillouin spectroscopy can measure the adipose tissues’ stiffness, which increases in obesity. Samples of brown and white adipose tissues obtained from control and diet-induced obese adult rats were analyzed. The results show that both adipose tissues of the obese group exhibit a greater high-frequency longitudinal elastic modulus than the control samples, and that the brown fat is generally stiffer than white adipose. The Raman spectra indicates that the lipids’ accumulation in adipose tissue outpaces the fibrosis, and that the high-fat diet has a greater effect on the brown adipose than the white fat. Overall, the powerful combination of Brillouin and Raman microspectroscopies successfully assessed both the mechanical properties and chemical composition of adipose tissue simultaneously for the first time. The results indicate that the adipose tissue experiences an obesity-induced increase in stiffness and lipid content, with the brown adipose tissue undergoing a more pronounced change compared to white adipose.

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“…The high-frequency longitudinal elastic modulus can, therefore, be calculated directly from the Brillouin shift for backscattered signal collection as

M^{'} = \frac{1}{4} ρ {(\frac{normalΔ ν_{B} λ_{0}}{n})}^{2}

Section: Introductionmentioning

confidence: 99%

Optical assessment of changes in mechanical and chemical properties of adipose tissue in diet‐induced obese rats

Troyanova‐Wood

Gobbell

Meng

et al. 2017

Journal of Biophotonics

Self Cite

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show abstract

“…Reports of Brillouin applications in biology include characterization of β-amyloid plaques 21 , 22 and atherosclerotic plaque 23 in mouse models, melanoma diagnosis 24 , 25 , and a wide range of experiments in the ophthalmological field, in which the technique shows great promise. Clinically viable devices for in vivo assessment of cornea stiffness have already been tested in humans 26 , 27 .…”

Section: Introductionmentioning

confidence: 99%

Assessment of myocardial viscoelasticity with Brillouin spectroscopy in myocardial infarction and aortic stenosis models

Villalba-Orero

Riobóo

Gontán

et al. 2021

Sci Rep

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Heart diseases are associated with changes in the biomechanical properties of the myocardial wall. However, there is no modality available to assess myocardial stiffness directly. Brillouin microspectroscopy (mBS) is a consolidated mechanical characterization technique, applied to the study of the viscoelastic and elastic behavior of biological samples and may be a valuable tool for assessing the viscoelastic properties of the cardiac tissue. In this work, viscosity and elasticity were assessed using mBS in heart samples obtained from healthy and unhealthy mice (n = 6 per group). Speckle-tracking echocardiography (STE) was performed to evaluate heart deformation. We found that mBS was able to detect changes in stiffness in the ventricles in healthy myocardium. The right ventricle showed reduced stiffness, in agreement with its increased compliance. mBS measurements correlated strongly with STE data, highlighting the association between displacement and stiffness in myocardial regions. This correlation was lost in pathological conditions studied. The scar region in the infarcted heart presented changes in stiffness when compared to the rest of the heart, and the hypertrophied left ventricle showed increased stiffness following aortic stenosis, compared to the right ventricle. We demonstrate that mBS can be applied to determine myocardial stiffness, that measurements correlate with functional parameters and that they change with disease.

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“…Other eye pathologies that result in mechanical changes, like glaucoma, could also benefit from the development of ocular Brillouin devices. Additional pathologies that might benefit from Brillouin microscopy are cardiovascular diseases 48 and several cancer types 94,95 as mechanical differences have already been shown to be relevant for prognosis and overall On the other hand, the shear (G) and Young (E) moduli (red) refer to volume conserving deformations. As a consequence, in biological matter similarly to other liquids, gels or colloidal suspensions, K and M assume values in the GPa range at any frequencies, while G and E are almost vanishing (kPa range) for quasi-static deformations (low frequencies) where the system reacts as a liquid.…”

Section: Future Potential Of Brillouin Microscopy In Biology and Medi...mentioning

confidence: 99%

Brillouin microscopy: an emerging tool for mechanobiology

et al. 2019

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The role and importance of mechanical properties of cells and tissues in cellular function, development as well as disease has widely been acknowledged, however standard techniques currently used to assess them exhibit intrinsic limitations. Recently, a new type of optical elastography, namely Brillouin microscopy, has emerged as a nondestructive, label-and contact-free method which can probe the viscoelastic properties of biological samples with diffraction-limited resolution in 3D. This has led to increased attention amongst the biological and medical research communities, but also to debates about the interpretation and relevance of the measured physical quantities. Here, we review this emerging technology by describing the underlying biophysical principles and discussing the interpretation of Brillouin spectra arising from heterogeneous biological matter. We further elaborate on the technique's limitations as well as its potential for new insights in biology in order to guide interested researchers from various fields.

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Elasticity-based identification of tumor margins using Brillouin spectroscopy

Cited by 7 publications

References 29 publications

Optical assessment of changes in mechanical and chemical properties of adipose tissue in diet‐induced obese rats

Optical assessment of changes in mechanical and chemical properties of adipose tissue in diet‐induced obese rats

Assessment of myocardial viscoelasticity with Brillouin spectroscopy in myocardial infarction and aortic stenosis models

Brillouin microscopy: an emerging tool for mechanobiology

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