Elasticity boundary conditions required for cell mechanotaxis on microelastically-patterned gels

Topography Design Concept of a Tissue Engineering Scaffold for Controlling Cell Function and Fate Through Actin Cytoskeletal Modulation

“…A wide variety of cell types, such as human MSCs, 61,105,106 mouse ES cells, 107 fibroblasts, 18,108,109 and glioma cells, 110 have been reported to respond to the ECM elasticity within the range of in vivo tissue elastic modulus from 0.1 kPa in the brain to 100 kPa in pre-calcified bone. 111 This property of cells can be used to specify strength of the cytoplasm-ECM link (Fig.…”

Electroporation of adherent cells with low sample volumes on a microscope stage

“…This suggests that the new device may allow electroporation for cells on various types of substrate, such as microstructured surface (Nikkhah et al, 2012), nanofibrous substrate (Tutak et al, 2013) and gels with different stiffness (Kawano and Kidoaki, 2011).…”

2D-DIGE Proteomic Analysis of Mesenchymal Stem Cell Cultured on the Elasticity-tunable Hydrogels

“…For preparation of the gel, photocurable styrenated gelatin (StG) synthesized in-house was employed (Kidoaki and Matsuda, 2008;Kawano and Kidoaki, 2011). A sol solution of phosphate buffer saline (PBS) including StG (30% w/w; degree of derivatization: 100%) and water-soluble carboxylated camphorquinone (Okino et al, 2002) (0.1% w/v of gelatin) was spread between the vinyl-silanized glass substrate and the poly(N-isopropylacrylamide) coated glass substrate, and was irradiated by visible light with intensity of 100 mW/cm 2 (measured at 488 nm) for 40-360 s. The detached gels were immersed overnight with gently rocking in PBS for complete swelling of the hydrogels.…”

“…A sol solution of phosphate buffer saline (PBS) including StG (30% w/w; degree of derivatization: 100%) and water-soluble carboxylated camphorquinone (Okino et al, 2002) (0.1% w/v of gelatin) was spread between the vinyl-silanized glass substrate and the poly(N-isopropylacrylamide) coated glass substrate, and was irradiated by visible light with intensity of 100 mW/cm 2 (measured at 488 nm) for 40-360 s. The detached gels were immersed overnight with gently rocking in PBS for complete swelling of the hydrogels. Surface elasticity on each hydrogel was determined by microindentation analysis as previously described (Kawano and Kidoaki, 2011). The force-indentation curves of the gel surface were measured using Atomic Force Microscope (AFM) (NVB100; Olympus Optical Co. Ltd., Tokyo, Japan; AFM controller and software, Nanoscope IIIa; Veeco Instruments, CA, USA) with a silicon-nitride cantilever with a half pyramidal tip and nominal spring constant of 0.02 N/m at 10 randomly selected points (n=10) of 3 different samples (n=3).…”

“…The differential expressions of MSCs cultured on the cell adhesive elasticitytunable gels (as we have previously developed (Kidoaki and Matsuda, 2008;Kawano and Kidoaki, 2011)) were studied using the 2D-DIGE. DIGE-based proteomics has been applied for the study of the relative differences in protein abundance of human osteoprogenitor in response to surface topographies (Kantawong et al, 2009a(Kantawong et al, , 2009b(Kantawong et al, , 2009c, demonstrating the advantage of DIGE as a powerful tool for quantitative proteomics study of scarce samples.…”