Elasticity of Purified Gelatin Jellies as a Function of Hydrogen-Ion Concentration

Sheppard, S. E.; Sweet, S. S.; Benedict, Anber J.

doi:10.1021/ja01430a003

Cited by 10 publications

(8 citation statements)

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“…Poisson’s ratio ν was assumed to be 0.5 because the gelatin gel was considered incompressible, as has been previously reported. 34 The cantilever properties were defined by the opening angle α (=34°) and cantilever spring constant κ. A thermal vibration based program installed in the AFM instrument was used to calculate κ.…”

Section: Materials and Methodsmentioning

confidence: 99%

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Increasing Elasticity through Changes in the Secondary Structure of Gelatin by Gelation in a Microsized Lipid Space

et al. 2018

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Even though microgels are used in a wide variety of applications, determining their mechanical properties has been elusive because of the difficulties in analysis. In this study, we investigated the surface elasticity of a spherical microgel of gelatin prepared inside a lipid droplet by using micropipet aspiration. We found that gelation inside a microdroplet covered with lipid membranes increased Young’s modulus E toward a plateau value E* along with a decrease in gel size. In the case of 5.0 wt % gelatin gelled inside a microsized lipid space, the E* for small microgels with R ≤ 50 μm was 10-fold higher (35–39 kPa) than that for the bulk gel (∼3 kPa). Structural analysis using circular dichroism spectroscopy and a fluorescence indicator for ordered beta sheets demonstrated that the smaller microgels contained more beta sheets in the structure than the bulk gel. Our finding indicates that the confinement size of gelling polymers becomes a factor in the variation of elasticity of protein-based microgels via secondary structure changes.

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Section: Materials and Methodsmentioning

confidence: 99%

“…The elastic modulus ( E ) and Poisson’s ratio (ν) together define the material properties. Poisson’s ratio ν was assumed to be 0.5 because the gelatin gel was considered incompressible, as has been previously reported . The cantilever properties were defined by the opening angle α (=34°) and cantilever spring constant κ.…”

Section: Materials and Methodsmentioning

confidence: 99%

Increasing Elasticity through Changes in the Secondary Structure of Gelatin by Gelation in a Microsized Lipid Space

et al. 2018

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“…Gelatin is well-known to be quite elastic and incompressible, and its Poisson's ratio was assumed to be 0.5. 33 Similarly, the Poisson's ratio for alginate was assumed to be 0.5, based on work done by Wang et al 34 When glass is used as the substrate, the loading curve in the indentation tests can give an estimation of the elastic modulus of the hydrogels. Glass is rigid and has a high elastic modulus (generally on the order of tens of GPa), which is much greater than that of a hydrogel.…”

Section: ■ Results and Discussionmentioning

confidence: 99%

“…Gelatin is well-known to be quite elastic and incompressible, and its Poisson’s ratio was assumed to be 0.5 . Similarly, the Poisson’s ratio for alginate was assumed to be 0.5, based on work done by Wang et al…”

Section: Resultsmentioning

confidence: 99%

Underwater Contact Behavior of Alginate and Catechol-Conjugated Alginate Hydrogel Beads

2017

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Modifying hydrogels with catechol functionality is a promising approach for improving their mechanical and interfacial properties in water, particularly in biological environments. However, the effects of this modification on hydrogels' contact behavior with soft tissues are not well-studied due to the complexity of hydrogels and lack of suitable techniques to probe this behavior. In addition, modification can alter the mechanical properties of hydrogels, resulting in consequences for adhesive strength as well. In this work, we report an investigation of the contact behavior of alginate hydrogels with and without conjugation of catechol functionality, aiming to elucidate the role of catechol modification on wet adhesion of alginates to a model tissue-like material, gelatin. To directly characterize this soft-on-soft contact, which has commonly been a challenge, we developed an indentation-based contact adhesion measurement using alginate hydrogel beads as the testing probe. We found that <3% conjugation of catechol can significantly improve the adhesion of alginate to gelatin by half an order of magnitude, with this adhesion depending heavily on contact time and pH. In contrast, the reduced elastic modulus from modification resulted in lower adhesive strength on rigid substrates. These findings provide valuable insight into the effects of catechol modification of hydrogels, especially in their interaction with tissue-like soft substrates, as well as a simple method for the direct measurement of time-and pH-dependent hydrogel adhesion behavior underwater.

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“…Lisbonne and Vielquin (rS) and Friche, et al ( r r) employed electrodialysis to separate and study enzymes. Shepard, et al (23) purified gelatin by electrodialysis and Bechhold and Rosenberg (2) followed the same method to purify both gelatin and glue. Foster and Schmidt (9) used electrodialysis to separate hexone bases from certain proteins.…”

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confidence: 99%

Electrodialysis of Soil: 1. A Study of the Method¹

Humfeld¹,

Alben

1927

Agronomy Journal

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A preliminary report on the study of base exchange m soil by electrodialysis, using a modification of the Mattson cell has been s·ubmitted for publication (6) 3 and should appear at an early date. It is the purpose of this paper to present the experimental data secured in the work referred to and to give more detailed information regarding the technic of the method.Reference has been made previously to the work of Hiss1nk ( r 5) and Gedroiz (r2) on base exchange and to that of Cameron and Bell (4), Konig and co-workers (q ), and Mattson (2o) on the electrodialysis of minerals and soils.The principle of electrodialysis has found application in the past along various lines. Dhere and Gorgeleuski (7), Adolf and Pauli (r), Berilara and Beaver (3), and others (8, IO,22) have used electrodialysis on blood serums with various objects in view. Lisbonne and Vielquin (rS) and Friche, et al ( r r) employed electrodialysis to separate and study enzymes. Shepard, et al (23) purified gelatin by electrodialysis and Bechhold and Rosenberg (2) followed the same method to purify both gelatin and glue. Foster and Schmidt (9) used electrodialysis to separate hexone bases from certain proteins. Taylor, et al (24) studied the effect of electrodialysis on insulin. Harvey (r3) in 1925 and Hoffman and Gortner (r6) in the same year purified agar by electrodialysis. Heyman (r4) in 1925 found that electro-. dialysis was about roo times as efficient as either dialysis or electroendosmosis in removing electrolytes from colloidal solutions. Moore, et al (2r) employed electrodialysis as a means of studying the biochemical differences in abnormal apple tissue. Mattson (2o) recently applied electrodialysis to the determination of the replaceable bases in colloids obtained from soils and found a very close agreement between the results secured and those obtained by the use of norma\ ammonium chloride.The cell used by Mattson consisted of three chambers, the center one being separated from the two outside ones by parchment paper.

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Elasticity of Purified Gelatin Jellies as a Function of Hydrogen-Ion Concentration

Cited by 10 publications

References 0 publications

Increasing Elasticity through Changes in the Secondary Structure of Gelatin by Gelation in a Microsized Lipid Space

Increasing Elasticity through Changes in the Secondary Structure of Gelatin by Gelation in a Microsized Lipid Space

Underwater Contact Behavior of Alginate and Catechol-Conjugated Alginate Hydrogel Beads

Electrodialysis of Soil: 1. A Study of the Method¹

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Elasticity of Purified Gelatin Jellies as a Function of Hydrogen-Ion Concentration

Cited by 10 publications

References 0 publications

Increasing Elasticity through Changes in the Secondary Structure of Gelatin by Gelation in a Microsized Lipid Space

Increasing Elasticity through Changes in the Secondary Structure of Gelatin by Gelation in a Microsized Lipid Space

Underwater Contact Behavior of Alginate and Catechol-Conjugated Alginate Hydrogel Beads

Electrodialysis of Soil: 1. A Study of the Method1

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Electrodialysis of Soil: 1. A Study of the Method¹