Elastin Barrier Membranes for Guided Tissue Regeneration Technologies

Abstract

This article discusses the prospects for the use of new elastin barrier membranes manufactured using adapted technologies for the selective isolation of the elastin component from the extracellular xenogenic matrix of the pericardium ligamentous apparatus: (1) by high-temperature extraction under pressure; (2) cyanogen bromide method. A commercial material, Geistlich Bio–Gide® membrane (BG), was used as a control comparison group. It is shown that the materials of group (1) have a high degree of biocompatibility, exceeding the indicators of the control group BG. Based on the results of an study in a model of subcutaneous heterotopic implantation in rats, it was shown that elastin BM has a chemoattractant effect on the mesenchymal recipient cells and, unlike the control, is able to integrate to a high degree into the surrounding recipient tissues. At the same time, the materials of group (1) had a pronounced proangiogenic effect. Thus, it has been shown that elastin BM groups (1) have a medium-term barrier function and are able to induce full-fledged cellular repopulation and local neoangiogenesis, which can be useful in clinical practice, primarily in GTR technologies (with gingival flap augmentation) or when used together with other BM as an angiogenesis inducer to ensure formation of the vascular bed in GBR technologies of bone tissue.

A comprehensive in vitro characterization of non-crosslinked, diverse tissue-derived collagen-based membranes intended for assisting bone regeneration

“…Since enzymatically-catalysed hydrolytic degradation has been recognized as the main cause for premature membrane resorption, sample sensitivity to type I collagenase was evaluated [ 8 , 9 , 18 , 46 , 47 ]. Consistently with less hydration and porosity that are expected to reduce exposure to the enzyme, membrane 2 was the most resistant.…”

“…These latter features are key to preserve the membrane’s structural integrity and ability to maintain space separation as long as needed for the underlying bone tissue regeneration. In addition, membranes that can biochemically prompt bone regeneration would be highly attractive [ 3 – 11 , 16 – 18 ]. All these aspects of the clinical performance are directly related to membrane features such as chemical composition, swelling, mechanical properties, porosity, thickness, surface morphological features, sensitivity to chemical and enzymatically-catalyzed degradation, specific interaction with cells from the host tissue.…”

Viability of Collagen Matrix Grafts Associated with Nanohydroxyapatite and Elastin in Bone Repair in the Experimental Condition of Ovariectomy

“…It is established in the literature that mineralized collagen offers advantages to the cellular chemotaxis process [85,86], in addition to providing greater longevity to the scaffolds [25]. The inclusion of proteins and growth factors also represents a promising alternative, but errors in dosimetry are associated with osteolysis, ectopic bone formation and carcinogenesis.…”

“…Five of these parallel chains form a microfibril 1 cm long and 1 mm in diameter with 67 nm bands, where the deposition of hydroxyapatite, the main inorganic constituent of the osteoid matrix secreted by osteoblasts, subsequently occurs [23]. Despite significant advances in collagen synthesis and purification processes, there is still no scaffold that fully reproduces the properties of the native protein; there are some minimal differences remaining in its structure [24] and it is susceptible to hydrolytic and enzymatic degradation if it comes into contact with body fluid [25]. The proteins also have low osteoinductivity [26], which favors premature resorption and the loss of mechanical stability [27], and for these reasons, other components are often added to increase useful life and resistance and obtain greater osteogenic potential [25][26][27].…”

Fabrication of Periodontal Membrane From Nelumbo nucifera: A Novel Approach for Dental Applications

“…Current limitations of these membranes include weak biocompatibility of nonabsorbable and absorbable synthetic polymer membranes [9]. Furthermore, it should be noted that natural collagen membranes demonstrating rapid degradation rates possess restricted rigidity and may not effectively sustain barrier functionality [10,11]. To improve biocompatibility, possible solutions include using electrospinning techniques, nanofiber scaffolds, or developing functional gradient membranes [12].…”