Elastomechanical properties of bovine veins

Rossmann, Jenn Stroud

doi:10.1016/j.jmbbm.2009.06.003

Cited by 25 publications

(37 citation statements)

References 27 publications

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“…The longitudinal E1 of native bovine veins (0.1±0.02 MPa) reported by Rossmann (40) is comparable to our E1 values of decellularized (0.1±0.06 MPa) and cross-linked (0.98±0.1 MPa) bovine veins. Moreover, the longitudinal TS of decellularized (1.2±0.7 MPa) and crosslinked (3.9±0.6 MPa) bovine veins produced higher results than the native veins value (0.5±0.1 MPa) reported by Rossmann (40), indicating an increased collagen fiber strength. Therefore, decellularized and mostly cross-linked veins showed significantly increased mechanical characteristics with respect to the non-cross-linked ones.…”

Section: Discussionmentioning

confidence: 74%

Decellularized bovine reinforced vessels for small-diameter tissue-engineered vascular grafts

Grandi

Baiguera²,

Martorina³

et al. 2011

Int J Mol Med

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Abstract. The aim of the present study was to investigate the influence of a decellularization protocol on the structure and the mechanical behavior of small-diameter (<6 mm) tibial calf arteries and veins. Calf vessels were decellularized by a detergent-enzymatic method (DEM), partially hydrolyzed with trypsin and subsequently cross-linked using poly(ethylene glycol) diglycidyl ether. Our results showed that i) the DEM can be considered a simple and valuable procedure for the preparation of complete acellular arteries and veins able to preserve a high degree of collagen and elastic fibers, and ii) poly(ethylene glycol) diglycidyl ether cross-linking treatment provides appropriate mechanical reinforcement of blood vessels. Histologically, the decellularized vessels were obtained employing the detergent-enzymatic procedure and their native extracellular matrix histoarchitecture and components remained well preserved. Moreover, the decellularization protocol can be considered an effective method to remove HLA class I antigen expression from small-diameter tibial calf arteries and veins. Cytocompatibility of decellularized cross-linked vessels was evaluated by endothelial and smooth muscle cell seeding on luminal and adventitial vessel surfaces, respectively.

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Section: Discussionmentioning

confidence: 74%

Decellularized bovine reinforced vessels for small-diameter tissue-engineered vascular grafts

Grandi

Baiguera²,

Martorina³

et al. 2011

Int J Mol Med

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“…Generally, in a tensile test, a sample is attached to the force transducer by a clamping [8,9,10], pin or staple loading [10,11,12]. However, it is difficult to attach cell sheet by conventional fixtures from two reasons.…”

Section: B Design Of Self-attachable Fixturementioning

confidence: 99%

“…The ranges of the maximum tension applied to the cell sheets were 51.5 ± 55.9 N (control) and 67.7 ± 102.0 N (FBS-). We found that the maximum strain of cell sheets (strain: 0.50 -0.78) was smaller than that of single cells (strain: 0.5 -3) [7,8] and we considered that the difference was the contribution of initial strain of the cell sheets. In previous studies, single cells detached from the substrate were contracting and so cells were free from initial strain.…”

Section: F Protocol Of Tensile Testmentioning

confidence: 99%

“…First, it is difficult to attach the cell sheet in the test system by conventional fixtures due to the mechanical fragility of the sheet. In the tensile test of tissues or biomaterials, specimens are attached to the test system by clamping [8,9,10], or using pins or staples [11,12,13]. These methods cannot be used for cell sheets; for the clamp type fixture, the cell sheet may be squashed by the stress of clamping and for pin or staple type fixtures, the cell sheet will tear at the through holes used for the hooking pins or staples.…”

Section: Introductionmentioning

confidence: 99%

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Measurement system for biomechanical properties of cell sheet

Uesugi

Akiyama

Hoshino

et al. 2013

2013 IEEE/RSJ International Conference on Intelligent Robots and Systems

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Abstract² In this study, we present a new fixture (self-attachable fixture) and tensile test system for measuring mechanical properties of cell sheet. To evaluate strength of cell sheet, it is the most important to measure mechanical properties of tensile mode. However, there has been no study which measured the tensile mechanical properties of cell sheet, since it has been difficult to attach a cell sheet in the tensile test system owing to the structure of the conventional fixture, and there has been no tensile test system which had a measurement range that covered the tension force range of the cell sheets. Therefore, we have addressed these problems by developing a self-attachable fixture and a tensile test system. By using developed system, we measured mechanical properties (tension, stress and initial stiffness) C2C12 of cell sheet cultured in different recipe of culture medium. The initial stiffness of cell sheet cultured in culture medium without FBS had a tendency to become stiffer. This indicates that our new fixture and test system are applicable for evaluating mechanical properties of cell sheets.

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“…In the second method, tensiometers (universal testing machine) are employed to determine blood vessel's biomechanical properties. For instance, Balaz and Rossman utilized this method for lumbar and jugular veins for porcine and bovine coronary arteries, respectively [7,8].…”

Section: Introductionmentioning

confidence: 99%

Measurement of mechanical properties of human saphenous vein using an inflation experiment

Forouzandeh

Bozorgi

Meshkat

et al. 2013

2013 20th Iranian Conference on Biomedical Engineering (ICBME)

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Coronary Artery Diseases are one of the main reasons of mortality. When these arteries occlude, usually a Coronary Artery Bypass Graft (CABG) surgery is performed. Sine human Saphenous Veins (SV) are used for CABG, they are of interest for researchers. In this study human SV samples undergo inflation test, using an inflation test device. Displacements of the samples for different pressures are analyzed, and average values are used as input of a computational method. In the numerical simulation the tissue is assumed as an elastic, isotropic, and homogenous solid material, and its output is Young's Modulus (E) of the tissue. Results show that E of the SV increases linearly with the distension pressure. Although simplifications were applied in this study, it can be helpful for giving a basic insight about mechanical properties of human Saphenous Vein, which can be followed by more realistic studies in the future.

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Elastomechanical properties of bovine veins

Cited by 25 publications

References 27 publications

Decellularized bovine reinforced vessels for small-diameter tissue-engineered vascular grafts

Decellularized bovine reinforced vessels for small-diameter tissue-engineered vascular grafts

Measurement system for biomechanical properties of cell sheet

Measurement of mechanical properties of human saphenous vein using an inflation experiment

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