Electric field directed layer-by-layer assembly of horseradish peroxidase nanotubes via anodic aluminum oxide template

Wu, Fanghua; Hu, Zhichao; Wang, Liwei; Xu, Jingjing; Xian, Yuezhong; Tian, Yuan; Jin, Litong

doi:10.1016/j.elecom.2008.01.020

Cited by 15 publications

(13 citation statements)

References 19 publications

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“…So a well-biocompatible and negatively-charged HRP-PMB was obtained through oppositely charged adsorption [24]. The HRP-PMB-AAO/ITO electrode was prepared then based on the positively-charged AAO [11] and negatively-charged HRP-PMB.…”

Section: Resultsmentioning

confidence: 99%

“…Wang et al has reported a novel DNA biosensor based on singlestranded DNA (ssDNA) probe functionalized aluminum anodized oxide (AAO) nanopore membranes [10]. Wu et al has studied the synthesis and characterization of horseradish peroxidase (HRP) nanotubes with high density and well order using anodic aluminum oxide (AAO) as templates, in combination with EFDLA deposition technique [11]. Therefore, nanoporous anodic aluminum oxide template has been shown to be ideal for biosensor applications, as its unique ordered nano-arrays structure.…”

Section: Introductionmentioning

confidence: 99%

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Amperometric biosensor for hydrogen peroxide based on the co-electrodeposition of horseradish peroxidase and methylene blue on an ITO electrode modified with an anodic aluminum oxide template

Liu

Sun

et al. 2011

Microchim Acta

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We report on a nano-array sensor for hydrogen peroxide (H 2 O 2 ) that is based on a nanoporous anodic aluminum oxide template. This was used as a matrix for the co-immobilization of horseradish peroxidase (HRP) and methylene blue (MB) on the surface of an indium tin oxide electrode. The immobilized HRP retained its natural activity and MB is capable of efficiently shuttle electrons between HRP and the electrode. The new electrode was characterized by SEM and electrochemical methods. It exhibits fast response, long-term stability, high sensitivity and good selectivity to H 2 O 2 . Under optimized conditions, it linearly responds to H 2 O 2 in the concentration range from 1.0 μM to 26 mM, with a detection limit of 0.21 μM (at S/N03).

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Amperometric biosensor for hydrogen peroxide based on the co-electrodeposition of horseradish peroxidase and methylene blue on an ITO electrode modified with an anodic aluminum oxide template

Liu

Sun

et al. 2011

Microchim Acta

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“…The process of the electrophoretic deposition promoted the efficiency of DNA delivery, increased the duration of the release of DNA from polyelectrolyte multilayers, 19 enhanced the pervaporation performances of polyelectrolyte multilayers for separating isopropanol-water mixtures, 20,21 and rapidly fabricated functional protein nanoarrays. 22 The adsorption of polyelectrolytes was weakened at an intermediate electric field strength because of the electrolysis of the water at the electrode surface. However, under a strong electric field, reinforced electrophoretic deposition of the polyelectrolyte chains overwhelmed the effects of the electrolysis of water.…”

Section: Introductionmentioning

confidence: 99%

The fast deposition of antireflection coating

Wang

Peng

et al. 2017

J Coat Technol Res

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In this investigation, the effect of deposition time (5, 60, or 300 s) on a double-sided antireflection (AR) coating done by layer-by-layer deposition of polyelectrolyte was determined. The different structures and transmittance of an AR coating induced by electric-field-assisted layer-by-layer deposition (ELBLD) (with voltage of 0.5, 5, 50, or 500 V) and normal deposition (with voltage of 0 V) were compared. The electric field was perpendicular to the glass surface. After 5.5 bilayers of PAH/PAA were fabricated, the film was exposed to an acidic treatment of HCl solution (pH 2.4) for 65 s, followed by a brief rinse ($15 s) in deionized water, blown dry with air, and heat treated at 220°C for 2 h. Regardless of whether the electric field was applied, the average transmittance of visible light of the AR coating was more than 96% except for the reduced transmittance because of the excessive deposition of polyelectrolyte at an applied voltage of 500 V and a deposition time of 300 s. Average transmittance of 97.68 ± 0.31% was obtained at normal deposition of 5.5 bilayers with deposition time of 60 s. The polyelectrolytes had a propensity to form larger particles or accumulate into bundles under ELBLD, which was detrimental to the transmittance of an AR coating. Therefore, at the same deposition time, transmittance values of ELBLD samples were slightly lower than those of normal deposition. In other words, in the overall performance, the AR films of normal deposition are superior to those of ELBLD. The blowing pressure applied to dry the coating had significant impact on the quality of the film.

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“…In addition, because deposition primarily depends on electrostatic attractions, weak polyelectrolytes demonstrate low adsorption efficiency. Therefore, electrophoretic deposition has been developed to accelerate deposition and increase loading efficiency [4][5][6][7][8]. Compared with conventional LbL assemblies in which polyelectrolytes are suspended in solutions for passive adsorption, electric fields can be used to guide the movement of charged materials toward electrodes, leading to significant reductions in required deposition times.…”

Section: Introductionmentioning

confidence: 99%

“…applying positive and negative biases during the deposition of CdTe and PDDA, respectively. Although electric field assistance (EFA) has been broadly used to enhance LbL assembly of protein multilayers for biological application [5,6,12], applications to gene delivery are rare. Dieguez et al [13] deposited DNA with poly-l-lysine (PLL) as multilayers using constant positive electrical biases, and the ensuing electrochemical reactions on the electrodes altered PEMs structure and improved delivery efficiency.…”

Section: Introductionmentioning

confidence: 99%

Electrophoretic deposition to promote layer-by-layer assembly for in situ gene delivery application

Zheng

2015

Colloids and Surfaces B: Biointerfaces

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Electric field directed layer-by-layer assembly of horseradish peroxidase nanotubes via anodic aluminum oxide template

Cited by 15 publications

References 19 publications

Amperometric biosensor for hydrogen peroxide based on the co-electrodeposition of horseradish peroxidase and methylene blue on an ITO electrode modified with an anodic aluminum oxide template

Amperometric biosensor for hydrogen peroxide based on the co-electrodeposition of horseradish peroxidase and methylene blue on an ITO electrode modified with an anodic aluminum oxide template

The fast deposition of antireflection coating

Electrophoretic deposition to promote layer-by-layer assembly for in situ gene delivery application

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