Electric field-directed migration of mesenchymal stem cells enhances their therapeutic potential on cisplatin-induced acute nephrotoxicity in rats

Abstract

Cisplatin is widely used as an anti-neoplastic agent but is limited by its nephrotoxicity. The use of mesenchymal stem cells (MSCs) for the management of acute kidney injury (AKI) represents a new era in treatment but effective homing of administered cells is needed. This study aimed to investigate the effect of bone marrow-derived mesenchymal stem cells (BM-MSCs) on cisplatin-induced AKI in rats after directed migration by electric field (EF). Forty-eight adult male albino rats were equally classified into four groups: control, cisplatin-treated, cisplatin plus BM-MSCs, and cisplatin plus BM-MSCs exposed to EF. Serum levels of IL-10 and TNF-α were measured by ELISA. Quantitative real-time PCR analysis for gene expression of Bcl2, Bax, caspase-3, and caspase-8 was measured. Hematoxylin and eosin (H&E) staining, periodic acid Schiff staining, and immunohistochemical analysis were also done. MSC-treated groups showed improvement of kidney function; increased serum levels of IL-10 and decreased levels of TNF-α; and increased mRNA expression of Bcl2 and decreased expression of Bax, caspase-3, and caspase-8 proteins comparable to the cisplatin-injured group. EF application increased MSCs homing with significant decrease in serum urea level and caspase-3 gene expression together with significant increase in Bcl2 expression than occurred in the MSCs group. Restoration of normal kidney histomorphology with significant decrease in immunohistochemical expression of caspase-3 protein was observed in the BM-MSCs plus EF group compared to the BM-MSCs group. EF stimulation enhanced the MSCs homing and improved their therapeutic potential on acute cisplatin nephrotoxicity.

Ameliorative Effect of Taurine on Nandrolone Decanoate Induced Toxicity on Brain, Heart and Testis in Adult Male Albino Rats

“…At the end of the experiment (24 hours from the last dose) and under complete anesthesia, all animals were euthanized with 25 mg/kg of sodium thiopental IP injection (Abdelrahman et al, 2023). Venous blood samples were sampled from the retro-orbital plexus of animals using micro-capillary glass tubes (Abdelaal et al, 2018), the samples were centrifuged and the supernatant fluid was kept in -20 °C till used for biochemical analysis.…”

The remodeling of ovarian function: targeted delivery strategies for mesenchymal stem cells and their derived extracellular vesicles

“…For example, low-intensity pulsed ultrasound (LIPUS) stimulation promotes the expression of hAD-MSCs chemokine receptors, especially CXCR4, and to some extent increases the number of hAD-MSCs homing to CTX-injured ovaries [ 68 ]. In other disease areas, the homing ability to cisplatin-induced damaged kidneys was enhanced in BMSCs exposed to electric field [ 69 ].…”