Electric Field Stimulation of Precision-Cut Lung Slices.

Abstract

Tag der mündlichen Prüfung: 06. Oktober 2011 Diese Dissertation ist auf den Internetseiten der Hochschulbibliothek online verfügbar. AcknowledgementThe

Airway Contractility in the Precision-Cut Lung Slice after Cryopreservation

“…An attractive new preparation for studying reactivity of the small airway is the precision-cut lung slice (PCLS) (15)(16)(17)(18)(19)(20)(21)(22)(23)(24).…”

Comparison Of Airway Responses In Sheep Of Different Age In Precision-cut Lung Slices (PCLS)

“…In this context of note, the current experiments were exclusively performed on small airways, since they were less than 2 mm in diameter. Therefore, our results reflect the situation only for the peripheral lung reducing interslice variations since airway responses can differ between large and small airways [23,29].…”

“…airways and vessels embedded in the parenchyma, remains intact and permit physiological measurements [23][24][25][26]. Even nerves remain functional and can be specifically activated [29]. In the past, PCLS from rat, mouse, guinea pig, non-human primate and human lung have been investigated [14,26].…”

“…This is an important difference compared to rodent airways that do not or at best very weakly respond to LTD 4 [15]. Leukotrienes contribute to airway obstruction in many human asthmatics [29]. In this regard sheep PCLS, in particular newborn, may appear as a suitable animal model to study bronchoconstriction.…”

“…As described before [29], EFS of PCLS was carried out in standard 12-well plates at a reaction volume of 1 mL standard MEM. The PCLS were placed in between two platinum electrodes of 12 mm distance and were mounted by a Teflon ring.…”