Charge transport properties of organic semiconductors critically depend on their molecular packing structures. Controlling the charge transport in varying the molecular packing and understanding their structure-property correlations are essential for developing high-performance organic electronic devices. Here we demonstrate that the charge carrier mobility in organic single-crystal nanowires can be modulated with respect to the intermolecular center-to-center distance by applying uniaxial strain to the cofacially stacked crystals. Monotonic changes in charge carrier mobility (from 0.0196 to 19.6 cm 2 V -1 s -1 for 6,13-bis(triisopropylsilylethylnyl) pentacene (TIPS-PEN)) were observed under a wide range of strains from -16.7% (compressive) to 16.7% (tensile). Furthermore, the measured values of charge carrier mobility were in good agreement with theoretical calculations based on charge localized hopping theory. These results provide a definitive relationship between intermolecular packing arrangement and charge transports, which enables a huge improvement in charge carrier mobility for organic single-crystal materials.