Electrical characterization of DNA molecules in solution using impedance measurements

Liu, Yi Shao; Banada, Padmapriya P.; Bhattacharya, Shantanu; Bhunia, Arun K.; Bashir, Rashid

doi:10.1063/1.2908203

Cited by 49 publications

(25 citation statements)

References 12 publications

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“…Changes in wall capacitance result in change in the imaginary component of the measured impedance. 32 Similar mechanisms are at play for proteins resulting in a decrease in impedance as protein concentration increases at the electrode surface.…”

Section: A Capacitance Modulationmentioning

confidence: 99%

“…32 The je sol is the relative dielectric permittivity of the solution and is explicitly affected by DNA dipole moment (DNA length and charge) and concentration of the DNA. The changes in the extracted wall capacitance C w , which represents the DNA capacitance due to the movement of the counterioncharges around the DNA backbone (dielectric relaxation of DNA dipoles), varied with changes in concentration of our DNA solutions.…”

Section: A Capacitance Modulationmentioning

confidence: 99%

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Label-free electronic probing of nucleic acids and proteins at the nanoscale using the nanoneedle biosensor

Esfandyarpour

Javanmard²,

Koochak

et al. 2013

Biomicrofluidics

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Detection of proteins and nucleic acids is dominantly performed using optical fluorescence based techniques, which are more costly and timely than electrical detection due to the need for expensive and bulky optical equipment and the process of fluorescent tagging. In this paper, we discuss our study of the electrical properties of nucleic acids and proteins at the nanoscale using a nanoelectronic probe we have developed, which we refer to as the Nanoneedle biosensor. The nanoneedle consists of four thin film layers: a conductive layer at the bottom acting as an electrode, an oxide layer on top, and another conductive layer on top of that, with a protective oxide above. The presence of proteins and nucleic acids near the tip results in a decrease in impedance across the sensing electrodes. There are three basic mechanisms behind the electrical response of DNA and protein molecules in solution under an applied alternating electrical field. The first change stems from modulation of the relative permittivity at the interface. The second mechanism is the formation and relaxation of the induced dipole moment. The third mechanism is the tunneling of electrons through the biomolecules. The results presented in this paper can be extended to develop low cost point-of-care diagnostic assays for the clinical setting. V C 2013 AIP Publishing LLC. [http://dx

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Section: A Capacitance Modulationmentioning

confidence: 99%

Section: A Capacitance Modulationmentioning

confidence: 99%

Label-free electronic probing of nucleic acids and proteins at the nanoscale using the nanoneedle biosensor

Esfandyarpour

Javanmard²,

Koochak

et al. 2013

Biomicrofluidics

View full text Add to dashboard Cite

show abstract

“…The strategy relies on capacitance changes of electrodes patterned on a touch panel when they are placed in contact with DNA solutions, which exhibit capacitances that depend on their concentrations. [12][13][14] Because this projected capacitive touchscreen detects contact points of the capacitive materials with the electrode layer patterned on the touch panel, it can be activated by simply applying test samples to the touchscreen panel surface without the need for a human touching event. Furthermore, the projected capacitive touchscreen method should enable multiplexed detection because it simultaneously recognizes multiple touchdown events.…”

mentioning

confidence: 99%

A Touchscreen as a Biomolecule Detection Platform

Won

Park

2011

Angew Chem Int Ed

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“…Some groups have demonstrated detection and counting of cells by using a microfluidic integrated with electrode for various electrical measurement method in an application of food safety [10] and real-time monitoring bio-threat [11]. This measurement technique is based on the alteration of impedance across a measurement electrodes due to the deceasing of ionic current passing between electrodes when a presence of the cells.…”

Section: Introductionmentioning

confidence: 99%

A novel integrated dual microneedle-microfluidic impedance flow cytometry for cells detection in suspensions

Mansor

Takeuchi

Nakajima

et al. 2017

IJECE

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In this study, a new, simple and cost-effective impedance detection of yeast cell concentration by using a novel integrated dual microneedle-microfluidic impedance flow cytometry was introduced. The reported method for impedance flow cytometry detection utilizes embedded electrode and probe in the microfluidic device to perform measurement of electrical impedance when a presence of cells at sensing area. Nonetheless, this method requires costly and complicatedly fabrication process of electrode. Furthermore, to reuse the fabricated electrode, it also requires intensive and tedious cleaning process. Due to that, a dual microneedle integrated at the half height of the microchannel for cell detection as well as for electrical measurement was demonstrated. A commercial available Tungsten needle was utilized as a dual microneedle. The microneedle was easy to be removed from the disposable PDMS microchannel and can be reused with the simple cleaning process, such as washed by using ultrasonic cleaning. Although this device was low cost, it preserves the core functionality of the sensor, which is capable of detecting the passing cells at sensing area. Therefore, this device is suitable for low cost medical and food safety screening and testing process in developing countries.

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Electrical characterization of DNA molecules in solution using impedance measurements

Cited by 49 publications

References 12 publications

Label-free electronic probing of nucleic acids and proteins at the nanoscale using the nanoneedle biosensor

Label-free electronic probing of nucleic acids and proteins at the nanoscale using the nanoneedle biosensor

A Touchscreen as a Biomolecule Detection Platform

A novel integrated dual microneedle-microfluidic impedance flow cytometry for cells detection in suspensions

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