Electrical communication of cytochrome enriched Escherichia coli JM109 cells with graphite electrodes

Alferov, S. V.; Coman, Virginia; Gustavsson, Tobias; Reshetilov, A. N.; Wachenfeldt, Claes von; Hägerhäll, Cecilia; Gorton, Lo

doi:10.1016/j.electacta.2009.03.090

Cited by 29 publications

(24 citation statements)

References 32 publications

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“…63 Later examples by the same group showed that introduction of a longer side chain to the OsRP (Os-II, Chart 1) increased the flexibility of the polymer, allowing for improved contact between the redox active polymer and respiratory enzymes present in Pseudomonas species 64 and cytochromeenriched E. coli. 66 Electronic communication can also be established with Gram-positive microorganisms (e.g. Bacillus subtilis), despite their thicker cell walls.…”

Section: Semiconducting and Redox-active Polymersmentioning

confidence: 99%

Modification of Abiotic–Biotic Interfaces with Small Molecules and Nanomaterials for Improved Bioelectronics

Catania

Bazan

2013

Chem. Mater.

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Low efficiency of charge extraction (and conversely charge injection) across biotic-abiotic interfaces constitutes an obstacle to the integration of biological and electronic systems in high-performance bioelectronic devices. Advances in the promotion of charge transport across these typically non-conductive interfaces will have far-reaching implications in important applications such as alternative energy generation, bioelectrosynthesis, diagnostics, and environmental monitoring. This review highlights the use of synthetic materials to improve electrical interfacing between biological systems and electrodes, focusing specifically on whole cell bioelectrochemical systems. By taking advantage of a rich variety of materials chemistry and synthetic methodologies, significant improvements to the facilitation of charge transport across abiotic-biotic interfaces have been realized. The modifications of the bioelectronic interfaces presented herein include the use of organic small molecules, semiconducting and redox active polymers, inorganic nanoparticles, carbon nanotubes, graphene, hybrid organic-inorganic systems, and micro-/nanoelectrodes. However, design rules to guide material selection and choices regarding device architecture remain ambiguous. Establishment of a clearer understanding of bioelectronic charge transfer phenomena, their constituent pathways, and means of stimulating or selecting for different pathways is still work in progress. As such, great opportunities exist for materials scientists to contribute to these topics through design and implementation.

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Section: Semiconducting and Redox-active Polymersmentioning

confidence: 99%

Modification of Abiotic–Biotic Interfaces with Small Molecules and Nanomaterials for Improved Bioelectronics

Catania

Bazan

2013

Chem. Mater.

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“…Electronic interrogation of biological systems with redox molecules has allowed for detection of changes in cell metabolic activity171819, redox state202122, toxicity23 and other parameters4. Cells have been engineered for enhanced electron flow2425 and to allow for electronic detection of engineered cell activity2627. Electronic signals translated through redox molecules also show controlled glucose consumption28 and regulation of enzymatic activity29.…”

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confidence: 99%

Electronic control of gene expression and cell behaviour in Escherichia coli through redox signalling

et al. 2017

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The ability to interconvert information between electronic and ionic modalities has transformed our ability to record and actuate biological function. Synthetic biology offers the potential to expand communication ‘bandwidth' by using biomolecules and providing electrochemical access to redox-based cell signals and behaviours. While engineered cells have transmitted molecular information to electronic devices, the potential for bidirectional communication stands largely untapped. Here we present a simple electrogenetic device that uses redox biomolecules to carry electronic information to engineered bacterial cells in order to control transcription from a simple synthetic gene circuit. Electronic actuation of the native transcriptional regulator SoxR and transcription from the PsoxS promoter allows cell response that is quick, reversible and dependent on the amplitude and frequency of the imposed electronic signals. Further, induction of bacterial motility and population based cell-to-cell communication demonstrates the versatility of our approach and potential to drive intricate biological behaviours.

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“…To make electrical connections to cells, most approaches rely on introduction of noncellular redox species (10,11) or physical means to abrogate the inherently electrically insulating character of cellular membranes (12). Here we explore a radically different, biologically focused approach: to use synthetic biology to introduce an electron transfer pathway that routes electrons along a well-defined path from the cell interior to an extracellular inorganic material.…”

mentioning

confidence: 99%

Engineering of a synthetic electron conduit in living cells

Jensen¹,

Albers

Malley

et al. 2010

Proc. Natl. Acad. Sci. U.S.A.

261

246

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Engineering efficient, directional electronic communication between living and nonliving systems has the potential to combine the unique characteristics of both materials for advanced biotechnological applications. However, the cell membrane is designed by nature to be an insulator, restricting the flow of charged species; therefore, introducing a biocompatible pathway for transferring electrons across the membrane without disrupting the cell is a significant challenge. Here we describe a genetic strategy to move intracellular electrons to an inorganic extracellular acceptor along a molecularly defined route. To do so, we reconstitute a portion of the extracellular electron transfer chain of Shewanella oneidensis MR-1 into the model microbe Escherichia coli. This engineered E. coli can reduce metal ions and solid metal oxides ∼8× and ∼4× faster than its parental strain. We also find that metal oxide reduction is more efficient when the extracellular electron acceptor has nanoscale dimensions. This work demonstrates that a genetic cassette can create a conduit for electronic communication from living cells to inorganic materials, and it highlights the importance of matching the size scale of the protein donors to inorganic acceptors.cytochrome c | nanobioelectronics | synthetic biology | iron reduction | living-nonliving interfaces

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Electrical communication of cytochrome enriched Escherichia coli JM109 cells with graphite electrodes

Cited by 29 publications

References 32 publications

Modification of Abiotic–Biotic Interfaces with Small Molecules and Nanomaterials for Improved Bioelectronics

Modification of Abiotic–Biotic Interfaces with Small Molecules and Nanomaterials for Improved Bioelectronics

Electronic control of gene expression and cell behaviour in Escherichia coli through redox signalling

Engineering of a synthetic electron conduit in living cells

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