Electrical conductivity spectra of hepatic tumors reflect hepatocellular carcinoma progression in mice

Shetty, Shricharith; Anushree, U; Kumar, Rajesh; Bharati, Sanjay

doi:10.1088/2057-1976/abbbd5

Cited by 7 publications

(1 citation statement)

References 37 publications

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“…[75], [109]. In tissue analyses, like those performed by O'Rourke et al and Shetty et al, it was observed that tumors had higher conductivity than the surrounding liver, but supportive data is limited [66], [110]. Ultimately, extensive patient sampling is necessary to better understand presentations, particularly in the context of emerging pathophysiologies such as NAFLD.…”

Section: E Limitationsmentioning

confidence: 99%

Simulation of Image-Guided Microwave Ablation Therapy Using a Digital Twin Computational Model

Servin,

Collins,

Heiselman

et al. 2024

IEEE Open J. Eng. Med. Biol.

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Emerging computational tools such as healthcare digital twin modeling are enabling the creation of patient-specific surgical planning, including microwave ablation to treat primary and secondary liver cancers. Healthcare digital twins (DTs) are anatomically one-to-one biophysical models constructed from structural, functional, and biomarker-based imaging data to simulate patient-specific therapies and guide clinical decision-making. In microwave ablation (MWA), tissue-specific factors including tissue perfusion, hepatic steatosis, and fibrosis affect therapeutic extent, but current thermal dosing guidelines do not account for these parameters. This study establishes an MR imaging framework to construct three-dimensional biophysical digital twins to predict ablation delivery in livers with 5 levels of fat content in the presence of a tumor. Four microwave antenna placement strategies were considered, and simulated microwave ablations were then performed using 915 MHz and 2450 MHz antennae in Tumor Naïve DTs (control), and Tumor Informed DTs at five grades of steatosis. Across the range of fatty liver steatosis grades, fat content was found to significantly increase ablation volumes by approximately 29–l42% in the Tumor Naïve and 55–60% in the Tumor Informed DTs in 915 MHz and 2450 MHz antenna simulations. The presence of tumor did not significantly affect ablation volumes within the same steatosis grade in 915 MHz simulations, but did significantly increase ablation volumes within mild-, moderate-, and high-fat steatosis grades in 2450 MHz simulations. An analysis of signed distance to agreement for placement strategies suggests that accounting for patient-specific tumor tissue properties significantly impacts ablation forecasting for the preoperative evaluation of ablation zone coverage.

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Section: E Limitationsmentioning

confidence: 99%

Simulation of Image-Guided Microwave Ablation Therapy Using a Digital Twin Computational Model

Servin,

Collins,

Heiselman

et al. 2024

IEEE Open J. Eng. Med. Biol.

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Anticancer therapeutic potential of multimodal targeting agent- “phosphorylated galactosylated chitosan coated magnetic nanoparticles” against N-nitrosodiethylamine-induced hepatocellular carcinoma

Udupi,

Shetty,

Aranjani

et al. 2024

Drug Deliv. and Transl. Res.

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Superparamagnetic iron oxide nanoparticles (SPIONs) are extensively used as carriers in targeted drug delivery and has several advantages in the field of magnetic hyperthermia, chemodynamic therapy and magnet assisted radionuclide therapy. The characteristics of SPIONs can be tailored to deliver drugs into tumor via “passive targeting” and they can also be coated with tissue-specific agents to enhance tumor uptake via “active targeting”. In our earlier studies, we developed HCC specific targeting agent- “phosphorylated galactosylated chitosan”(PGC) for targeting asialoglycoprotein receptors. Considering their encouraging results, in this study we developed a multifunctional targeting system- “phosphorylated galactosylated chitosan-coated magnetic nanoparticles”(PGCMNPs) for targeting HCC. PGCMNPs were synthesized by co-precipitation method and characterized by DLS, XRD, TEM, VSM, elemental analysis and FT-IR spectroscopy. PGCMNPs were evaluated for in vitro antioxidant properties, uptake in HepG2 cells, biodistribution, in vivo toxicity and were also evaluated for anticancer therapeutic potential against NDEA-induced HCC in mice model in terms of tumor status, electrical properties, antioxidant defense status and apoptosis. The characterization studies confirmed successful formation of PGCMNPs with superparamagnetic properties. The internalization studies demonstrated (99–100)% uptake of PGCMNPs in HepG2 cells. These results were also supported by biodistribution studies in which increased iron content (296%) was noted inside the hepatocytes. Further, PGCMNPs exhibited no in vivo toxicity. The anticancer therapeutic potential was evident from observation that PGCMNPs treatment decreased tumor bearing animals (41.6%) and significantly (p ≤ 0.05) lowered tumor multiplicity. Overall, this study indicated that PGCMNPs with improved properties are efficiently taken-up by hepatoma cells and has therapeutic potential against HCC. Further, this agent can be tagged with 32P and hence can offer multimodal cancer treatment options via radiation ablation as well as magnetic hyperthermia. Graphical Abstract Schematic illustration of PGCMNPs synthesis, characterization and its anticancer potential: PGCMNPs were synthesized by co-precipitation method. The successful synthesis of PGCMNPs was confirmed by physical and chemical characterizations. PGCMNPs were biocompatible and exhibited no toxicity at tested parameters. PGCMNPs demonstrated higher uptake in HepG2 cells. The anticancer therapeutic potential of PGCMNPs in HCC mouse model, was evident from improved tumor statistics, increased low conductivity tumors and increased apoptosis mediated cell death.

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Anticancer therapeutic potential of phosphorylated galactosylated chitosan against N-nitrosodiethyl amine-induced hepatocarcinogenesis

Anushree

Shetty²,

Kulkarni³

et al. 2022

Archives of Biochemistry and Biophysics

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Electrical conductivity spectra of hepatic tumors reflect hepatocellular carcinoma progression in mice

Cited by 7 publications

References 37 publications

Simulation of Image-Guided Microwave Ablation Therapy Using a Digital Twin Computational Model

Simulation of Image-Guided Microwave Ablation Therapy Using a Digital Twin Computational Model

Anticancer therapeutic potential of multimodal targeting agent- “phosphorylated galactosylated chitosan coated magnetic nanoparticles” against N-nitrosodiethylamine-induced hepatocellular carcinoma

Anticancer therapeutic potential of phosphorylated galactosylated chitosan against N-nitrosodiethyl amine-induced hepatocarcinogenesis

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