Electrical detection of dengue virus (DENV) DNA oligomer using silicon nanowire biosensor with novel molecular gate control

Hashim, U.; Arshad, M. K. Md; Kasjoo, Shahrir R.; Rahman, Shaharin Fadzli Abd; Ruslinda, A. Rahim; Fathil, M. F. M.; Adzhri, R.; Shahimin, M. M.

doi:10.1016/j.bios.2016.04.033

Cited by 117 publications

(47 citation statements)

References 59 publications

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“…The hybridization between probe and target DNA was detected through variation in current, conductance, and resistance on genosensor because of negative charges which increased with the binding of probe and target DNA. The genosensor developed in this study successfully detected DNA with a LOD of 2.0 fM [34]. Ariffin et al employed nickel(II) salphen complex as DNA hybridization indicator for developing optical genosensor to detect DENV DNA.…”

Section: Detection Of Dengue Genomementioning

confidence: 90%

New Tools for Dengue Diagnostics

Kumari¹,

Deva²,

Lal³

et al. 2019

Dengue Fever - A Resilient Threat in the Face of Innovation

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Dengue caused by four antigenically distinct serotype remains a serious health concern around the world, particularly in the tropical areas. Clinical signs and symptoms of this disease are indistinguishable from other infectious disease; therefore, laboratory diagnosis is very crucial for confirming the disease that will be useful for the patient's management. In laboratory, dengue can be confirmed using cell culture, RNA detection, and serological detection based on ELISA and immunochromatographic test. However, each of these methods has certain practical limitations. Therefore, researchers from all over the world have been working to address these limitations. In this chapter, we will highlight the current research toward the development of novel point-of-care test for the diagnosis of dengue in acute and convalescent phase.

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Section: Detection Of Dengue Genomementioning

confidence: 90%

New Tools for Dengue Diagnostics

Kumari¹,

Deva²,

Lal³

et al. 2019

Dengue Fever - A Resilient Threat in the Face of Innovation

View full text Add to dashboard Cite

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“…A C C E P T E D ACCEPTED MANUSCRIPT 9 extremely high detection sensitivity of DNA sequences [35], proteins [36], and viruses [37]. A small feature size with a high surface-to-volume ratio enables the high sensitivity of the SiNW.…”

Section: Silicon Nanowirementioning

confidence: 99%

Progression in sensing cardiac troponin biomarker charge transductions on semiconducting nanomaterials

Fathil

Arshad

Ruslinda

et al. 2016

Analytica Chimica Acta

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“…The molecular-based diagnostic assays is preferred as it is more sensitive and can provide reliable results in shorter assay time compared to serological techniques [9] [10]. The hybridization technique for detection of viral nucleic acid has attracted the enormous efforts in the development of DNA biosensor [11] [12]. DNA biosensors consist of an immobilized DNA strand to detect the complementary sequence by hybridization process.…”

Section: A N U S C R I P Tmentioning

confidence: 99%

Enhanced sensing of dengue virus DNA detection using O2 plasma treated-silicon nanowire based electrical biosensor

Rahman

Yusof

Hashim

et al. 2016

Analytica Chimica Acta

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Dengue Virus (DENV) has become one of the most serious arthropod-borne viral diseases, causing death globally. The existing methods for DENV detection suffer from the late stage treatment due to antibodies-based detection which is feasible only after five days following the onset of the illness. Here, we demonstrated the highly effective molecular electronic based detection utilizing silicon nanowire (SiNW) integrated with standard complementary metal-oxide-semiconductor (CMOS) process as a sensing device for detecting deoxyribonucleic acid (DNA) related to DENV in an early stage diagnosis. To transform the fabricated devices as a functional sensing element, three-step procedure consist of SiNW surface modification, DNA immobilization and DNA hybridization were employed. The detection principle works by detecting the changes in current of SiNW which bridge the source and drain terminal to sense the immobilization of probe DNA and their hybridization with target DNA. The oxygen (O) plasma was proposed as an effective strategy for increasing the binding amounts of target DNA by modified the SiNW surface. It was found that the detection limit of the optimized O plasma treated-SiNW device could be reduced to 1.985 × 10 M with a linear detection range of the sequence-specific DNA from 1.0 × 10 M to 1.0 × 10 M. In addition, the developed biosensor device was able to discriminate between complementary, single mismatch and non-complementary DNA sequences. This highly sensitive assay was then applied to the detection of reverse transcription-polymerase chain reaction (RT-PCR) product of DENV-DNA, making it as a potential method for disease diagnosis through electrical biosensor.

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Electrical detection of dengue virus (DENV) DNA oligomer using silicon nanowire biosensor with novel molecular gate control

Cited by 117 publications

References 59 publications

New Tools for Dengue Diagnostics

New Tools for Dengue Diagnostics

Progression in sensing cardiac troponin biomarker charge transductions on semiconducting nanomaterials

Enhanced sensing of dengue virus DNA detection using O2 plasma treated-silicon nanowire based electrical biosensor

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