Electrical detection of DNA hybridization: Three extraction techniques based on interdigitated Al/Al2O3 capacitors

Moreno-Hagelsieb, L.; Foultier, Boris; Laurent, G.; Pampin, Rémi; Remacle, José; Raskin, J.-P.; Flandre, Denis

doi:10.1016/j.bios.2006.10.024

Cited by 66 publications

(20 citation statements)

References 12 publications

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“…And they have been used to enzyme analysis [10 -12], immune analysis [13 -15], nucleic acid analysis [16,17], drug analysis [18,19] and environment analysis [20].…”

Section: Introductionmentioning

confidence: 99%

Simultaneous Voltammetry Determination of Dihydroxybenzene Isomers by Nanogold Modified Electrode

Han

Zhang

2009

Electroanalysis

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A convenient electrochemical deposition method to prepare nanogold/glassy carbon modified electrode (nano-Au/ GCE) is adopted. In 0.1 mol/L HAc-NaAc buffer solution (pH 4.61), the nano-Au/GCE shows an excellent electrocatalytical behavior for the redox of dihydroxybenzene. A simple, rapid and highly selective voltammetry for simultaneous determination of dihydroxybenzene isomers, hydroquinone, catechol, and resorcinol, is developed using the nano-Au/GCE. This method has been applied to the direct determination of the three dihydroxybenzene isomers in artificial wastewater.

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“…And they have been used to enzyme analysis [10 -12], immune analysis [13 -15], nucleic acid analysis [16,17], drug analysis [18,19] and environment analysis [20].…”

Section: Introductionmentioning

confidence: 99%

Simultaneous Voltammetry Determination of Dihydroxybenzene Isomers by Nanogold Modified Electrode

Han

Zhang

2009

Electroanalysis

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“…Thus the suggested nanoparticle signal amplification was very efficient for ultrasensitive electrochemical detection of DNA hybridization. Compared to other electrochemical methods [42][43][44][45] without the signal amplification, this proposed nanoparticle-based method showed a much-lower limit of detection, and was competitive with the highly sensitive detection of DNA by amplifying the sample with PCR technique. [46] In addition, this method was superior to some amplification techniques, such as liposome (0.75 amol), [28] magnetic particles (5.7 fmol), [47] bio-barcodes (2.5 fmol L À1 ) [48] and Au-NP-based amplification (10 zmol).…”

Section: Stripping Voltammetric Analysis Of Target Dnamentioning

confidence: 99%

Quantum‐Dot‐Functionalized Poly(styrene‐co‐acrylic acid) Microbeads: Step‐Wise Self‐Assembly, Characterization, and Applications for Sub‐femtomolar Electrochemical Detection of DNA Hybridization

Dong

Yan

et al. 2010

Adv Funct Materials

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A novel nanoparticle label capable of amplifying the electrochemical signal of DNA hybridization is fabricated by functionalizing poly(styrene‐co‐acrylic acid) microbeads with CdTe quantum dots. CdTe‐tagged polybeads are prepared by a layer‐by‐layer self‐assembly of the CdTe quantum dots (diameter = 3.07 nm) and polyelectrolyte on the polybeads (diameter = 323 nm). The self‐assembly procedure is characterized using scanning and transmission electron microscopy, and X‐ray photoelectron, infrared and photoluminescence spectroscopy. The mean quantum‐dot coverage is (9.54 ± 1.2) × 103 per polybead. The enormous coverage and the unique properties of the quantum dots make the polybeads an effective candidate as a functionalized amplification platform for labelling of DNA or protein. Herein, as an example, the CdTe‐tagged polybeads are attached to DNA probes specific to breast cancer by streptavidin–biotin binding to construct a DNA biosensor. The detection of the DNA hybridization process is achieved by the square‐wave voltammetry of Cd2+ after the dissolution of the CdTe tags with HNO3. The efficient carrier‐bead amplification platform, coupled with the highly sensitive stripping voltammetric measurement, gives rise to a detection limit of 0.52 fmol L−1 and a dynamic range spanning 5 orders of magnitude. This proposed nanoparticle label is promising, exhibits an efficient amplification performance, and opens new opportunities for ultrasensitive detection of other biorecognition events.

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“…While this advance into the nano-regime could be viewed as incremental in the field of semiconductor electronics, its potential impact in biomolecular sciences and medicine could be revolutionary as it opens for the first time direct physical access to the elemental units of biosystems -individual biomolecules such as proteins, which are also on the nanometer size scale. This access allows unprecedented explorations of fascinating possibilities, one of which being the direct electronic interfacing of individual proteins with the man-made world [5][6][7][8][9] . Nano-featured platforms such as carbon nanotube (CNT) arrays, offering chemical-and size-compatibility with proteins, have been directly electrically contacted to redox enzymes and have been shown to promote direct electron transfer and form efficient bio-nanoelectronic transduction system [10][11][12][13] .…”

Section: Introductionmentioning

confidence: 99%

DNA-programmed protein-nanoelectronic transducer array

Withey

Kim

2008

SPIE Proceedings

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By incorporating DNA as addressable linkers, we can direct and coordinate the simultaneous, parallel self-assembling and binding of multiple different redox proteins to designated nanoelectrodes. As a result, we have formed a nanoelectronic-protein transducer array which is capable of real-time, multiplexed detection of several analytes in parallel. The sequence-specificity of DNA hybridization provides the means of encoding spatial address instruction to the otherwise random self-assembling process and enables the desired programmability, scalability, and renewability. Results of this study, under an AFOSR MURI program, demonstrate the feasibility of a new paradigm of biosensing: detection of not only the presence of target substances but also the realtime activities of multiple biomolecules. In this system, the conjugated biomolecules and nanoelectronic components provide the active monitoring and mediating functions in real time, and can be integrated en masse into large arrays in a silicon-based integrated circuit.

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Electrical detection of DNA hybridization: Three extraction techniques based on interdigitated Al/Al2O3 capacitors

Cited by 66 publications

References 12 publications

Simultaneous Voltammetry Determination of Dihydroxybenzene Isomers by Nanogold Modified Electrode

Simultaneous Voltammetry Determination of Dihydroxybenzene Isomers by Nanogold Modified Electrode

Quantum‐Dot‐Functionalized Poly(styrene‐co‐acrylic acid) Microbeads: Step‐Wise Self‐Assembly, Characterization, and Applications for Sub‐femtomolar Electrochemical Detection of DNA Hybridization

DNA-programmed protein-nanoelectronic transducer array

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