Electrical Field Effect on Peri-Implant Osteogenesis: A Histologic and Histomorphometric Study

Giannunzio, G.; Speerli, Rodolfo Carlos; Guglielmotti, M.B.

doi:10.1097/id.0b013e318166d800

Cited by 18 publications

(10 citation statements)

References 10 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A growing number of studies have demonstrated that external electric fields and applied currents could induce oste ogenesis, promote the consolidation of fracture callus and accelerate skeletal attachment. [17][18][19][20][21] Especially in orthopedics, electric stimulation devices have been used to promote healing of bony nonunion. [22,23] These studies strongly suggest the pre dominant role of the physiologically relevant electrical signals in directing wound healing.…”

mentioning

confidence: 99%

Built‐In Electric Fields Dramatically Induce Enhancement of Osseointegration

Liu

Zhang

Cao

et al. 2017

Adv Funct Materials

View full text Add to dashboard Cite

Rapid and effective osseointegration is a great challenge in clinical practice. Endogenous electronegative potentials spontaneously appear on bone defect sites and mediate healing. Thus, bone healing can potentially be stimulated using physiologically relevant electrical signals in implants. However, it is difficult to directly introduce physiologically relevant electric fields in bone tissue. In this study, built-in electric fields are established between electropositive ferroelectric BiFeO 3 (BFO) nanofilms and electronegative bone defect walls to trigger implant osseointegration and biological healing. Epitaxial growth technique is used to organize the crystal panel at an atomic scale, and ferroelectric polarization of BFO nanofilms matching the amplitude and direction of endogenous electric potentials on bone defect walls is achieved. In the presence of built-in electric fields, implants with BFO nanofilms with downward polarization (BFO+) show rapid and superior osseointegration in the rat femur. The mechanism of this phenotypic osteogenic behavior is further studied by protein adsorption and stem cell behavior in different time points. BFO+ promotes protein adsorption and mesenchymal stem cell (MSC) attachment, spreading, and osteogenic differentiation. Custom-designed PCR array examination shows sequentially initiated Ca 2+ signaling, cell adhesion and spreading, and PI3K-AKT signaling in MSCs. The results of this study provide a novel strategy for the development of implant surface modification technology.

show abstract

mentioning

confidence: 99%

Built‐In Electric Fields Dramatically Induce Enhancement of Osseointegration

Liu

Zhang

Cao

et al. 2017

Adv Funct Materials

View full text Add to dashboard Cite

show abstract

“…Although many attempts, such as hyperbaric oxygenation, electrical stimulation, low‐intensity pulsed ultrasound stimulation, and growth factors, have been made to accelerate bone formation, these approaches are associated with technical difficulties, speculative efficacy, and controversial safety profile . Fortunately, recent advances in regenerative medicine have enabled stem cell therapy to be a promising strategy to shorten the consolidation period and decrease potential complications in DO .…”

mentioning

confidence: 99%

Locally injection of cell sheet fragments enhances new bone formation in mandibular distraction osteogenesis: A rabbit model

Liu

Yao

et al. 2013

Journal Orthopaedic Research

View full text Add to dashboard Cite

Effective methods to shorten the treatment period of distraction osteogenesis (DO) are needed. To investigate whether injections of osteogenic bone marrow stromal cell (BMSC) sheet fragments could be used to facilitate new bone formation during DO, 30 rabbits underwent bilateral mandibular osteotomy and their mandibles were lengthened at a rate of 0.75 mm/12 h for 6 days after a 5-day latency period. There were three treatment groups (n ¼ 10 for each group): Serum-free medium, dissociated BMSCs, and BMSC sheet fragments. A local injection was conducted with a needle directly into the distracted areas immediately after distraction. Rabbits were sacrificed for examination at 3 and 6 weeks after injection. Gross examination, radiographic evaluation, and micro-CT scanning indicated a significant increase in bony union in the BMSC sheet fragment group, compared with the medium group and the dissociated cell group. The histomorphometric analysis showed more intensive bone formation in the sheet fragment group than the other two groups at each time point. Additionally, the peak load was significantly higher in the fragment group than those in the others. The results show that injection of BMSC sheet fragments promotes bone formation in DO and indicate a promising approach to shorten the treatment period of osteodistraction. #

show abstract

“…Various materials were examined for treating implants, including resorbed polymers (e.g., chitozan) in combination with proteins (BMP), which facilitated the cells’ interactions with the substrate (Minamide et al, 2005; Lopez-Lacomba et al, 2006; Middleton et al, 2007); polyglactin mesh with growing fibroblasts (Sparks et al, 2007); positively-charged phosphorylcholine, which that is beneficial for cells and, therefore, improves the biocompatibility of medical devices with tissue in the organism (Susin et al, 2008); and RGD peptides (Secchi et al, 2007). We applied extracellular proteins (collagen and fibrin) for the preliminary treatment of titanium implants, which was more adequate for the organism and, consequently, more effective for the migration of cells from tissue than chemical, physical, or polymer treatments (Cho, Jung, 2003; Reclaru et al, 2003; Serro and Saramago, 2003; Stentport and Johansson, 2003; Fujibayashi et al, 2004; Park et al, 2007; Shibli et al, 2007; Giannunzio et al, 2008). …”

Section: Resultsmentioning

confidence: 99%

“…Along with the application of various biological materials for improving the implant surface, studies were undertaken to evaluate physical factors that may facilitate the regeneration of bone tissue, e.g., various sterilization methods (Serro and Saramago, 2003), laser-treatment (Cho and Jung, 2003), electrical stimulation (Giannunzio et al, 2008), and titanium oxidation (Shibli et al, 2006).…”

mentioning

confidence: 99%

Cell cultivation on porous titanium implants with various structures

et al. 2010

View full text Add to dashboard Cite

The paper presents data on the cultivation of human dermal fibroblasts and rabbit mesenchymal stromal cells on two types of porous titanium implants, i.e., those with irregular pores formed by pressed titanium particles and those with regular pores formed by the cohesion of one-size titanium particles inside the implant. The goal of this study was to determine what type of titanium implant porosity ensured its strongest interaction with cells. Cells were cultivated on implants for 7 days. During this period, they formed a confluent monolayer on the implant surface. Cells grown on titanium implants were monitored by scanning electron microscopy. Fibroblasts interaction with implants depended on the implant porosity structure. On implants with irregular pores cells were more spread. On implants with regular pores fibroblasts enveloped particles and were only occasionally bound with neighboring particles by small outgrowths. There was no tight interaction of particles inside the implant. In implants formed by pressed particles, cells grow not only on surface, but also in the depth of the implant. Thus, we suppose that a tighter interaction of cells with the titanium implant and, supposedly, tissues with the implant in the organism will take place in the variant when the implant structure is formed by pressed titanium particles, i.e., cellular interaction was observed inside the implant. In implants with irregular pores, cells grew both on the surface and in the depth. Thus, cells exhibited more adequate interactions with irregular pore titanium implants in vitro and hopefully the same interaction will be true in tissues after the implantation of the prosthesis into the organism.

show abstract

Electrical Field Effect on Peri-Implant Osteogenesis: A Histologic and Histomorphometric Study

Abstract: The use of a device generating a positive/negative electrical field resulted in the presence of woven bone.

Cited by 18 publications

References 10 publications

Built‐In Electric Fields Dramatically Induce Enhancement of Osseointegration

Built‐In Electric Fields Dramatically Induce Enhancement of Osseointegration

Locally injection of cell sheet fragments enhances new bone formation in mandibular distraction osteogenesis: A rabbit model

Cell cultivation on porous titanium implants with various structures

Contact Info

Product

Resources

About