Electrical field induce mBMSCs differentiation to osteoblast via protein adsorption enhancement

Xian, Mengyue; Fang, Liming; Liu, Yili; Wei, Qiang; Li, Hao; Yu, Zhonghang; He, Xin; Wu, Gang

doi:10.1016/j.colsurfb.2021.112158

Cited by 8 publications

(6 citation statements)

References 35 publications

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“…It is well-known that amine groups can increase protein concentration per surface area in a short time, and therefore cells show a tendency to attach to the surface [8]. There are different assumptions for protein adsorption on the hydroxyl group decorated surfaces, but it seems that the existence of the groups does not affect cell viability results [13,14]. The cell viability results indicate that chitosan can provide the desired environment for fibroblast growth, but the difference is insignificant compared to a control group (Figure 2).…”

Section: Resultsmentioning

confidence: 99%

Proliferative and Antimicrobial Evaluation of the Benzalkonium Chloride Loaded Walnut Shell-Rich Chitosan Gels

Katı

Altuntaş

2022

Cumhuriyet Science Journal

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Tissue engineering studies combine cells, biomaterials, and biomolecules to mimic native tissue. The selection of appropriate materials for tissue engineering applications encourages best practices from the lab to clinical trials, and natural biomaterials have the potential to offer desired features for these applications. Material abundance, ease of the process, and biocompatibility are the first milestones to choosing a suitable material. Lignocellulose is one of the most promising biomaterials for its biocompatible, antioxidant, and biodegradable features and is the most abundant material in nature. A walnut shell-added chitosan gel was developed in this study by exploiting chitosan's desired properties, such as biocompatibility, biodegradability, and mechanical capabilities, which boosted cell proliferation. Furthermore, the gel system was reinforced with benzalkonium chloride (BAC), a well-known eye drop sterilizing agent. The hydrogels were subjected to Fourier-transform infrared spectroscopy (FTIR) analyses, and BAC-related signals were observed. The results of BAC-loaded hydrogels revealed that the viability of the primary fibroblasts was enhanced on the BAC-loaded gels compared to tissue culture polystyrene, but the difference was not found statistically significant. Yet, antibacterial activity results demonstrated that only BAC-loaded gel systems have solid antibacterial activity. Additionally, the fibroblasts had the strongest proliferation profile on the walnut shell-added chitosan hydrogels compared to other test groups, but the films' bactericidal activity of the hydrogels was not apparent. After revising the BAC and walnut shell concentrations in the hydrogels, the findings demonstrated that the injectable gel system could be used for cell transplantation in vitro and in vivo.

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Section: Resultsmentioning

confidence: 99%

Proliferative and Antimicrobial Evaluation of the Benzalkonium Chloride Loaded Walnut Shell-Rich Chitosan Gels

Katı

Altuntaş

2022

Cumhuriyet Science Journal

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“…MG-63 cells present the adhesion receptors for fibronectin, the α5β1 integrin subunit, which finds its prime target of fibronectin on the glass bottom [38,39]. It was found that BSA adsorption was higher during electrical stimulation with 1 V/cm for 1 h on composite hydrogels [40]. This could be correlated with higher cell adhesion and spreading and mBMSC cell genotype and phenotype changes in the direction of differentiated osteoblasts [40].…”

Section: Discussionmentioning

confidence: 98%

“…It was found that BSA adsorption was higher during electrical stimulation with 1 V/cm for 1 h on composite hydrogels [40]. This could be correlated with higher cell adhesion and spreading and mBMSC cell genotype and phenotype changes in the direction of differentiated osteoblasts [40]. The research group of Garcia found that the adsorption of complex (e.g., lysozyme) and soft proteins (e.g., BSA or IgG) on conductive substrates can be modulated by the application of an external electric potential at 800 mV [41].…”

Section: Discussionmentioning

confidence: 99%

Short-Time Alternating Current Electrical Stimulation and Cell Membrane-Related Components

Buenning,

Bielfeldt,

Nebe

et al. 2024

Applied Sciences

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Electrical stimulation (ES) and its effects on biological systems is an area of research in regenerative medicine. The focus here is on the mechanism of action of ES on cell membrane-related components. A short alternating current (AC) stimulation (10 min) was applied on suspended human MG-63 osteoblasts via a commercially available multi-channel system (IonOptix). The pulsed ES with 1 V or 5 V and frequencies of 20 Hz on cells was performed immediately after cell seeding. The in vitro investigations were conducted by microscopy, flow cytometry, and particle analysis via a Litesizer within 24 h. The short-time ES with the parameter 1 V and 20 Hz was beneficial for the process of cell attachment, which could be related to an enhanced deposition of fibronectin on the glass bottom from the protein-containing medium (10% FBS). The MG-63 cells’ spherical coat hyaluronan remained constant and did not contribute to this AC-triggered adhesion. In this context, the cells’ zeta potential also did not play a role. The membrane potential analyzed via DiBAC4(3) was unchanged. Only the aquaporin channel AQP 8 in the cell membrane was significantly enhanced. Suspended cells in an AC electric field were activated during their settlement, and the fibronectin adsorption on the bottom contributed to this effect but not the membrane-related components.

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“…PC12 cells exhibited improved adhesion under electrostimulation [ 129 ]. Electrostimulation increases production of vinculin, which forms focal sites and recruits other proteins to form adhesive plaques, and upregulates the genes encoding FAK, integrin, NCAM, and N-cadherin; while levels of FAK and integrin, which mediate cell-ECM interactions, were positively correlated, those of NCAM and N-cadherin, which respond to cell–cell conjunction, were negatively correlated [ 122 , 130 ].…”

Section: Effect Of Electrostimulation On Cellsmentioning

confidence: 99%

Advances in electroactive biomaterials: Through the lens of electrical stimulation promoting bone regeneration strategy

Luo,

Zhang,

Xiong

et al. 2024

Journal of Orthopaedic Translation

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Electrical field induce mBMSCs differentiation to osteoblast via protein adsorption enhancement

Cited by 8 publications

References 35 publications

Proliferative and Antimicrobial Evaluation of the Benzalkonium Chloride Loaded Walnut Shell-Rich Chitosan Gels

Proliferative and Antimicrobial Evaluation of the Benzalkonium Chloride Loaded Walnut Shell-Rich Chitosan Gels

Short-Time Alternating Current Electrical Stimulation and Cell Membrane-Related Components

Advances in electroactive biomaterials: Through the lens of electrical stimulation promoting bone regeneration strategy

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