Electrical impedance-based contractile stress measurement of human iPSC-Cardiomyocytes

Wang, Xian; Wang, Li; Dou, Wenkun; Huang, Zongjie; Zhao, Qili; Malhi, Manpreet; Maynes, Jason T.; Sun, Yu

doi:10.1016/j.bios.2020.112399

Cited by 18 publications

(12 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Verapamil also blocks human Ether-à-go-go-related gene (hERG) channel but shows a false-positive risk for QT prolongation . It is also known to cause a negative chronotropic and negative inotropic effect on cardiomyocytes. , The drug experiment is carried out on the 12th day of the maximum contraction of the cardiomyocytes to ensure the observability of the results of the drug toxicity test. The changes in the displacement of the cantilever array and the sensor signal output are recorded over a range of verapamil concentrations from 50 to 1000 nM.…”

Section: Resultsmentioning

confidence: 99%

Real-Time Monitoring of Changes in Cardiac Contractility Using Silicon Cantilever Arrays Integrated with Strain Sensors

et al. 2021

View full text Add to dashboard Cite

This paper proposes the use of sensor-integrated silicon cantilever arrays to measure drug-induced cardiac toxicity in real time. The proposed cantilever sensors, unlike the conventional electrophysiological methods, aim to evaluate cardiac toxicity by measuring the contraction force of the cardiomyocytes corresponding to the target drugs. The surface of the silicon cantilever consists of microgrooves to maximize the alignment and the contraction force of the cardiomyocytes. This type of surface pattern also helps in the maturation of the cardiomyocytes by increasing the sarcomere length. The preliminary characterization of the cantilever sensors was performed on the cantilever surface, with the cardiomyocytes seeded with a density of 1000 cells/mm 2 , and the cardiac contractility was measured as a function of the culture days. The change in the contraction force of the cardiomyocytes due to the drug concentration was successfully measured through the integrated strain sensor in the culture media. The reliability of the sensor-integrated cantilevers and the feasibility of their mass production ensure that they meet the practical requirements in the medical applications.

show abstract

Section: Resultsmentioning

confidence: 99%

Real-Time Monitoring of Changes in Cardiac Contractility Using Silicon Cantilever Arrays Integrated with Strain Sensors

et al. 2021

View full text Add to dashboard Cite

show abstract

“…Integrating electrical biosensors into in vitro cardiac models enables in situ and continuous characterization of contractile function. IDE biosensors have been used to achieve continuous measurement of cardiomyocyte contraction by detecting the contraction-induced impedance signal changes 203 , 204 . In addition, advances in flexible electronics and sensing materials have provided new opportunities to integrate flexible sensors onto heart-on-a-chip platforms for contractility measurement inside the incubator environment 52 , 120 , 126 , 205 .…”

Section: Discussionmentioning

confidence: 99%

Microengineered platforms for characterizing the contractile function of in vitro cardiac models

et al. 2022

Self Cite

View full text Add to dashboard Cite

Emerging heart-on-a-chip platforms are promising approaches to establish cardiac cell/tissue models in vitro for research on cardiac physiology, disease modeling and drug cardiotoxicity as well as for therapeutic discovery. Challenges still exist in obtaining the complete capability of in situ sensing to fully evaluate the complex functional properties of cardiac cell/tissue models. Changes to contractile strength (contractility) and beating regularity (rhythm) are particularly important to generate accurate, predictive models. Developing new platforms and technologies to assess the contractile functions of in vitro cardiac models is essential to provide information on cell/tissue physiologies, drug-induced inotropic responses, and the mechanisms of cardiac diseases. In this review, we discuss recent advances in biosensing platforms for the measurement of contractile functions of in vitro cardiac models, including single cardiomyocytes, 2D monolayers of cardiomyocytes, and 3D cardiac tissues. The characteristics and performance of current platforms are reviewed in terms of sensing principles, measured parameters, performance, cell sources, cell/tissue model configurations, advantages, and limitations. In addition, we highlight applications of these platforms and relevant discoveries in fundamental investigations, drug testing, and disease modeling. Furthermore, challenges and future outlooks of heart-on-a-chip platforms for in vitro measurement of cardiac functional properties are discussed.

show abstract

“…mitochondrial oxidative stress and arrhythmias [ 28 – 32 ]. Prior studies demonstrated power law behavior in investigations of heart (beat) rate variability, calcium load, and contractile stress in cardiomyocytes [ 33 – 37 ]. Studies have shown that aberrant cardiac systems exhibit a discernible change in the exponent of the power law measured for heart rate variability, which describes small variations in the interval between heart beats, in patients with myocardial infarction and coronary heart disease, and in heart transplant patients [ 29 , 38 – 41 ].…”

Section: Introductionmentioning

confidence: 99%

Pacemaker translocations and power laws in 2D stem cell-derived cardiomyocyte cultures

et al. 2022

View full text Add to dashboard Cite

Power laws are of interest to several scientific disciplines because they can provide important information about the underlying dynamics (e.g. scale invariance and self-similarity) of a given system. Because power laws are of increasing interest to the cardiac sciences as potential indicators of cardiac dysfunction, it is essential that rigorous, standardized analytical methods are employed in the evaluation of power laws. This study compares the methods currently used in the fields of condensed matter physics, geoscience, neuroscience, and cardiology in order to provide a robust analytical framework for evaluating power laws in stem cell-derived cardiomyocyte cultures. One potential power law-obeying phenomenon observed in these cultures is pacemaker translocations, or the spatial and temporal instability of the pacemaker region, in a 2D cell culture. Power law analysis of translocation data was performed using increasingly rigorous methods in order to illustrate how differences in analytical robustness can result in misleading power law interpretations. Non-robust methods concluded that pacemaker translocations adhere to a power law while robust methods convincingly demonstrated that they obey a doubly truncated power law. The results of this study highlight the importance of employing comprehensive methods during power law analysis of cardiomyocyte cultures.

show abstract

Electrical impedance-based contractile stress measurement of human iPSC-Cardiomyocytes

Cited by 18 publications

References 31 publications

Real-Time Monitoring of Changes in Cardiac Contractility Using Silicon Cantilever Arrays Integrated with Strain Sensors

Real-Time Monitoring of Changes in Cardiac Contractility Using Silicon Cantilever Arrays Integrated with Strain Sensors

Microengineered platforms for characterizing the contractile function of in vitro cardiac models

Pacemaker translocations and power laws in 2D stem cell-derived cardiomyocyte cultures

Contact Info

Product

Resources

About