In this paper, we present a novel sensitive ion-sensitive field-effect transistor (ISFET) membrane based on Ba 0:7 Sr 0:3 TiO 3 (BST)/SiO 2 fabricated by sputtering deposition. The proposed device exhibits a linear shift in acidic solutions in the pH range from 1 to 10. The device sensitivity was about 50 -55 mV/pH for different deposition times. We also examined the trapping behavior of the surface hydrated layer using the metal-insulator-semiconductor (MIS) structure. Results show that the hydration layer gives rise to stress polarity dependence of electron injection when immersed in pH buffer solutions. Injection from the gate electrode produces larger positive charges and interface state densities in contrast to the substrate injection, which causes simultaneous positive and negative charge trapping. A physical model that quantitatively describes the asymmetry associated with the hydrated diffusion layer is presented, and the temperature effects of BST/ SiO 2 ISFET devices in the range from 25 to 65 C were examined. We observed that pH sensitivity increases with increasing temperature. The temperature coefficient of sensitivity (TCS) can be divided into two different ranges: 0.08 mV/pH C between 25 and 45 C, and 0.57 mV/pH C between 45 and 65 C. A better thermal stability is produced in the 25 and 45 C range in comparison with other sensitive layers.