Electrical properties of subsynaptic and nonsynaptic membranes of horizontal cells in fish retina

Trifonov, Yu. A.; Byzov, A. L.; Chailahian, L.M.

doi:10.1016/0042-6989(74)90085-6

Cited by 66 publications

(27 citation statements)

References 16 publications

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“…It has been shown that horizontal cells in situ have a non-linear f-V relation with a negative conductance region after the synaptic input from photoreceptors is suppressed by bright light [74]. Therefore, it seems reasonable to suppose that the voltage-dependent membrane conductances found in solitary horizontal cells are present in normal horizontal cells.…”

Section: Membrane Conductance Of the Axon Terminalmentioning

confidence: 99%

“…The situation seems to hold in in situ cones as well: bath application of 5 mM GABA produced a 5 mV hyperpolarization in the dark membrane potential (-30 mV) of a green-sensitive cone in the carp retina [42]. 4 [74]. Today membrane currents can be fully analyzed with single horizontal cells isolated from the retina by enzymatic dissociation and primary tissue culture [17,54,63,64] (Fig.…”

Section: Feedback Hypothesismentioning

confidence: 99%

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The functional role of retinal horizontal cells.

Kaneko

1987

Jpn.J.Physiol

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Section: Membrane Conductance Of the Axon Terminalmentioning

confidence: 99%

Section: Feedback Hypothesismentioning

confidence: 99%

The functional role of retinal horizontal cells.

Kaneko

1987

Jpn.J.Physiol

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“…The photoreceptors of the vertebrate retina are so regularly arranged that a transretinal current pulse, flowing from the receptor side to the vitreal side, effectively depolarizes the cone terminals, activates transmitter release, and thereby generates a transient depolarization (EPSP) in the horizontal cells (TRIFONOV, 1968;BYZOV and TRIFONOV, 1968;TRIFONOV et al, 1974;KANEKO and SHIMAZAKI, 1976;WATANABE et al, 1978). The transient EPSP's thus evoked in the horizontal cells are shown in Fig.…”

Section: Methodsmentioning

confidence: 99%

GABA-mediated negative feedback from horizontal cells to cones in carp retina.

et al. 1982

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In the isolated, perfused retina of the carp, properties of the feedback pathway from horizontal cells to cones were investigated by means of electrophysiological and neuropharmacological methods. When horizontal cells were hyperpolarized by illumination with an annulus, a depolarizing synaptic potential was produced in cones at the center of the annulus, suggesting that horizontal cells receive inputs from cones and exert a negative feedback to cones. On the other hand, a hyperpolarizing response (IPSP) was detected in cones, when a transient depolarization (EPSP) was induced in horizontal cells by application of a transretinal current pulse, which activated the release of transmitter from the photoreceptor terminals. The IPSP was abolished when the retina was perfused with a GABA-containing Ringer solution, because of desensitization of the feedback synapse. GABA also hyperpolarized the cone membrane, indicating the presence of a GABA-sensitive site in the cone. These results suggest that the GABA-mediated negative feedback operates from horizontal cells to cones in the dark, and ceases its function in the light.

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“…However, using a special method of uniform polarization of electrically coupled horizontal cells, Trifonov and his colleagues have demonstrated that horizontal cells in vivo have a non-linear I-V relationship with a negative conductance region after the synaptic transmission between photoreceptors and horizontal cells is suppressed by bright light (Trifonov, Byzov & Chailahian, 1974;Byzov, Trifonov, Chailahian & Golubtzov, 1977). Therefore, it seems reasonable to suppose that the active properties shown in solitary horizontal cells are present in normal horizontal cells, but suppressed in the presence of transmitters by some mechanism(s): for example, transmitters released from photoreceptors and/or interplexiform cells may modulate the ionic conductance(s) and suppress the active properties; intracellular and extracellular ionic composition may be changed under the influence of transmitters, resulting in the partial inactivation of the ionic conductance(s); a feed-back system from horizontal cells to photoreceptors may prevent potential jumps.…”

Section: Function Of the Ca Currentmentioning

confidence: 99%

Ionic currents of solitary horizontal cells isolated from goldfish retina.

Tachibana¹

1983

The Journal of Physiology

139

123

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SUMMARY1. Solitary horizontal cells, dissociated from papain-treated goldfish retinas, produce action potentials and show a non-linear current-voltage relationship. Underlying ion-conductance mechanisms were analysed by a single-micro-electrode voltage-clamp technique. Pharmacological and ion-substitution experiments revealed that ionic currents could be separated into at least four voltage-dependent currents: a Ca current and three types of K currents.2. The Ca current was activated by membrane depolarization beyond -45 mV, reached a maximal value near 0 mV, and became smaller at more positive potentials. By extrapolation, the reversal potential was estimated to be approximately + 50 mV. The Ca current was inactivated by accumulation of intracellular Ca ions but not by membrane depolarization. Co ions (4 mM) blocked this current. 3. The first type of K current showed anomalous (inward-going) rectification near the resting potential (--60 mV). Hyperpolarization from the resting level produced a large, almost steady inward current, while depolarization evoked only a small, steady outward current. The current-voltage relationship revealed a shallow negative resistance region at membrane potentials beyond -50 mV. The current was blocked by Cs (10 mM) or Ba (1 mM) ions.4. The second type of K current (the transient outward current) was activated by membrane depolarization beyond -25 mV. The peak amplitude increased almost exponentially as the membrane was depolarized. During steady depolarization this current decayed exponentially (time constant -500 ms at +20 mV). The current was inactivated by conditioning depolarization (>10 s) beyond -30 mV and blocked by 4-aminopyridine (10 mM).5. The third type of K current was the maintained outward current which was activated by membrane depolarization beyond -20 mV, increased to a steady level in a few hundred milliseconds, and showed little inactivation. The amplitude increased as the membrane was depolarized. The current was blocked by tetraethylammonium ions (20 mM).6. A Ca-mediated K current was not detected. 7. Action potentials and the non-linear current-voltage relationship of solitary

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Electrical properties of subsynaptic and nonsynaptic membranes of horizontal cells in fish retina

Cited by 66 publications

References 16 publications

The functional role of retinal horizontal cells.

The functional role of retinal horizontal cells.

GABA-mediated negative feedback from horizontal cells to cones in carp retina.

Ionic currents of solitary horizontal cells isolated from goldfish retina.

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