Electrical stimulation induces direct reprogramming of human dermal fibroblasts into hyaline chondrogenic cells

Lee, Gyu Seok; Kim, Min Gu; Kwon, Hyuck Joon

doi:10.1016/j.bbrc.2019.04.027

Cited by 20 publications

(13 citation statements)

References 33 publications

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“…In this context, recent studies have shown that human dermal fibroblasts can also be directly reprogrammed into hyaline chondrogenic cells by electrical stimulation. Lee et al were able to show that electrical stimulation with a frequency of 5.0 Hz and an electric field strength of 5 V/cm (applied with a commercial system) could enhance expression of chondrogenic markers, such as type II collagen, aggrecan, and Sox9 by a concomitant decrease of type I collagen without the addition of exogenous growth factors or gene transduction [43]. Considering this in further studies, it would be interesting to determine the release of specific growth factors following electrical stimulation to identify additional important pathways influenced by electric fields.…”

Section: Discussionmentioning

confidence: 99%

Re-Differentiation Capacity of Human Chondrocytes in Vitro Following Electrical Stimulation with Capacitively Coupled Fields

Krueger

Achilles

Zimmermann

et al. 2019

JCM

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Treatment of cartilage lesions remains a clinical challenge. Therefore, biophysical stimuli like electric fields seem to be a promising tool for chondrocytic differentiation and treatment of cartilage lesions. In this in vitro study, we evaluated the effects of low intensity capacitively coupled electric fields with an alternating voltage of 100 mVRMS (corresponds to 5.2 × 10−5 mV/cm) or 1 VRMS (corresponds to 5.2 × 10−4 mV/cm) with 1 kHz, on human chondrocytes derived from osteoarthritic (OA) and non-degenerative hyaline cartilage. A reduction of metabolic activity after electrical stimulation was more pronounced in non-degenerative cells. In contrast, DNA contents in OA cells were significantly decreased after electrical stimulation. A difference between 100 mVRMS and 1 VRMS was not detected. However, a voltage-dependent influence on gene and protein expression was observed. Both cell types showed increased synthesis rates of collagen (Col) II, glycosaminoglycans (GAG), and Col I protein following stimulation with 100 mVRMS, whereas this increase was clearly higher in OA cells. Our results demonstrated the sensitization of chondrocytes by alternating electric fields, especially at 100 mVRMS, which has an impact on chondrocytic differentiation capacity. However, analysis of further electrical stimulation parameters should be done to induce optimal hyaline characteristics of ex vivo expanded human chondrocytes.

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Section: Discussionmentioning

confidence: 99%

Re-Differentiation Capacity of Human Chondrocytes in Vitro Following Electrical Stimulation with Capacitively Coupled Fields

Krueger

Achilles

Zimmermann

et al. 2019

JCM

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“…These processes result in a flux of different ions across the plasma membrane [ 18 ]. Altered ion concentration leads to the activation of different gene expression [ 19 ], production and secretion of growth and transcription factors [ 16 , 20 ], cell adhesion [ 21 ] and cell-cell interaction molecules [ 22 ]. Typically, low electric fields are used in applying ES, which leads to moderate change in the membrane potential by a tenth of mV and initiates the movement of voltage-sensing domains, resulting in conformational changes and the opening of voltage-gated ion channels ( Figure 1 ).…”

Section: Electrical Stimulation Overviewmentioning

confidence: 99%

Electrical Stimulation in Cartilage Tissue Engineering

et al. 2023

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Electrical stimulation (ES) has been frequently used in different biomedical applications both in vitro and in vivo. Numerous studies have demonstrated positive effects of ES on cellular functions, including metabolism, proliferation, and differentiation. The application of ES to cartilage tissue for increasing extracellular matrix formation is of interest, as cartilage is not able to restore its lesions owing to its avascular nature and lack of cells. Various ES approaches have been used to stimulate chondrogenic differentiation in chondrocytes and stem cells; however, there is a huge gap in systematizing ES protocols used for chondrogenic differentiation of cells. This review focuses on the application of ES for chondrocyte and mesenchymal stem cell chondrogenesis for cartilage tissue regeneration. The effects of different types of ES on cellular functions and chondrogenic differentiation are reviewed, systematically providing ES protocols and their advantageous effects. Moreover, cartilage 3D modeling using cells in scaffolds/hydrogels under ES are observed, and recommendations on reporting about the use of ES in different studies are provided to ensure adequate consolidation of knowledge in the area of ES. This review brings novel insights into the further application of ES in in vitro studies, which are promising for further cartilage repair techniques.

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“…Lee et al found that human dermal fibroblasts (HDF) may be changed directly to hyaline chondrogenic cells under ES [24]. ES induces condensation of HDFs and rises levels of chondrogenic marker expression, such as type Ⅱ collagen, aggrecan, and Sox9, while simultaneously reducing expression levels of type I collagen when applied at 5 V/cm and 10 Hz of varied length.…”

Section: In Vitro Studiesmentioning

confidence: 99%

Some Electrical Stimulation Methods for Articular Cartilage Regeneration

Shi¹

2023

HSET

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Bioelectrical signals can regulate a wide range of cellular activities in living organisms, including division, differentiation, etc. The physiological properties of stem cell cells are regulated by artificial input of electrical stimuli, including electric and electromagnetic fields, to promote cartilage repair by inducing stem cell cellular differentiation toward cartilage. Electrical stimulation (ES) stimulates cartilage regeneration at the cellular level through three mechanisms: intricate interactions of the physical environment, growth factors (GFs), and signal transduction cascades. The relevant ways in which bioelectrical stimulation regulates cellular function are the subject of this review. The non-invasive nature of this and the fact that it is not dependent on exogenous growth factors offer great promise for the clinical application of ES. However, the precise mechanisms underlying how ES interacts with cells are not well understood and need a lot more investigation. Similarly, there is a certain variability in the means and parameters of ES in in vivo and in vitro experiments, which poses a great challenge for clinical applications. Here some feasible means of ES and specific ES parameters to provide ideas for subsequent clinical applications are reviewed.

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Electrical stimulation induces direct reprogramming of human dermal fibroblasts into hyaline chondrogenic cells

Cited by 20 publications

References 33 publications

Re-Differentiation Capacity of Human Chondrocytes in Vitro Following Electrical Stimulation with Capacitively Coupled Fields

Re-Differentiation Capacity of Human Chondrocytes in Vitro Following Electrical Stimulation with Capacitively Coupled Fields

Electrical Stimulation in Cartilage Tissue Engineering

Some Electrical Stimulation Methods for Articular Cartilage Regeneration

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