Electrical stimulation of adipose-derived mesenchymal stem cells in conductive scaffolds and the roles of voltage-gated ion channels

Zhang, Jieyu; Li, Min; Kang, En‐Tang; Neoh, K. G.

doi:10.1016/j.actbio.2015.12.024

Cited by 156 publications

(166 citation statements)

References 56 publications

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“…12,[58][59][60] In this work, calcium deposition and ALP activity were enhanced by exploiting HA, whereas electrical stimulation caused slightly more calcium deposition and ALP activity when applied in combination with HA (Fig. 12,[58][59][60] In this work, calcium deposition and ALP activity were enhanced by exploiting HA, whereas electrical stimulation caused slightly more calcium deposition and ALP activity when applied in combination with HA (Fig.…”

Section: Discussionmentioning

confidence: 68%

“…Previously, direct stimulation, capacitive-coupling, and inductive-coupling methods were utilized to deliver electrical stimulus with dissimilar effects on osteoblasts and mesenchymal stem cells. [7][8][9][10][11][12][13] Among conductive polymers, polypyrrole, polyaniline, and polythiophenes have been extensively utilized in biomedical engineering as biosensors, neural probes, tissue engineering scaffolds, drug delivery carriers, and so on. On the other hand, inductive-coupling inhibited cell growth and enhanced mineralization and expression of BMP-2 and transforming growth factor beta 1 (TGFb-1).…”

Section: Introductionmentioning

confidence: 99%

“…[7][8][9][10][11][12][13] Among conductive polymers, polypyrrole, polyaniline, and polythiophenes have been extensively utilized in biomedical engineering as biosensors, neural probes, tissue engineering scaffolds, drug delivery carriers, and so on. 12,[22][23][24] Chitosan (a natural polymer) has been widely used in bone engineering because of its biocompatibility, biodegradability, similarity to glycosaminoglycans (the main components of bone ECM) and the capability to enhance osteogenesis. [16][17][18] On the other hand, several disadvantages such as nonbiodegradability, low flexibility, and difficulty of processing limit its application.…”

Section: Introductionmentioning

confidence: 99%

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Sequential application of mineralized electroconductive scaffold and electrical stimulation for efficient osteogenesis

Oftadeh

Bakhshandeh

Dehghan

et al. 2018

J Biomedical Materials Res

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Osteogenic differentiation is enhanced by many inductive factors including biochemical agents, biomechanical stresses, and electrical stimulation. Regularly studies have focused on one factor at a time, while synergies can promote more effective and functional osteogenesis. Herein, for the first time, functional synergism between application of electrical stimulation and HA nanoparticles was evaluated in osteogenic differentiation. Prepared electrospun biocompatible conductive scaffold by amalgamating chitosan, aniline-pentamer, and hydroxyapatite incorporation was seeded by human bone-marrow-derived mesenchymal stem cells. The cells seeded on the scaffolds with and without hydroxyapatite were exposed to electrical stimulation and subsequently, osteogenic molecular markers and related signaling pathways were investigated. In general, all investigated osteogenic markers (osteocalcin, alkaline phosphatase, osteonectin, and Runx2) were upregulated transcriptionally in the cells seeded on the chitosan-embedded scaffolds. Separate utilization of electrical stimulation or hydroxyapatite-enhanced osteogenesis, while the cells exposed to both stimulators simultaneously, expressed higher levels of some of osteogenic genes significantly. Considering the functions and the positions of the markers in osteogenic signaling pathways, it can be concluded that HA might cooperate in the allocation of stem cells to osteoprogenitors in the early phase of osteogenesis while electrical stimulation helps committed cells with maturation and acquiring functional phenotypes. Altogether investigation of the synergism between different stimulators and exploiting the interactions in an optimized manner could lead to more efficient osteogenesis protocol for effective bone regeneration and tissue engineering. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 106A: 1200-1210, 2018.

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Section: Discussionmentioning

confidence: 68%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Sequential application of mineralized electroconductive scaffold and electrical stimulation for efficient osteogenesis

Oftadeh

Bakhshandeh

Dehghan

et al. 2018

J Biomedical Materials Res

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“…Previous investigators have shown that electrical fields can induce ion channel activation and elevate intracellular Ca 2+ concentrations (Bergh, Xu, & Farach-Carson, 2004;Zhang, Li, Kang, & Neoh, 2016). One reasonable explanation is EMF-induced cellular calcium oscillations.…”

Section: The Primary Cilium As An Electromagnetic Field Sensormentioning

confidence: 99%

Novel functions of the primary cilium in bone disease and cancer

Shi

Zhang

et al. 2019

Cytoskeleton

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The primary cilium, a sensory organelle that emanates from the cell surface of most mammalian cell types during growth arrest, has attracted the attention of many researchers over the past decade. Recently, a large number of new findings have assigned novel functions and roles to the primary cilium in signal transduction and related diseases, which has greatly augmented the importance of the cilium in human health and development. Here, we review emerging evidence supporting the primary cilium as a sensory organelle in signal transduction in microgravity, electromagnetic field sensing, chemosensation and tumorigenesis. We also present an overview of signal transduction crosstalk associated with the primary cilium in bone disease and cancer, including primary cilium-related Ca 2+ signaling, parathyroid hormone signaling, cAMP signaling, BMP/Smad1/5/8 signaling and Wnt signaling. We anticipate that emerging discoveries about the function of the primary cilium will provide novel insight into the molecular mechanisms of stimulus sensation, signal transduction and pathogenesis. K E Y W O R D Sbone disease, cancer, cytoskeleton, primary cilium, signal transduction

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“…(26) It can thus be beneficial to modulate growth factor release post-implantation using external stimuli. The usage of external physical stimuli to modulate behavior of tissue engineering scaffolds has gained popularity in recent years,(44–46) though few approaches have applied this methodology so far to 3DP scaffolds. One very interesting approach by Gupta et al utilized 3D-printed PLGA shells doped with plasmonic gold nanorods (AuNRs) to achieve photothermally mediated rupture of the shells and subsequent release of a payload from the aqueous core (Figure 1).…”

Section: Fabricating Spatiotemporal Growth Factor Patternsmentioning

confidence: 99%

Spatiotemporal control of growth factors in three-dimensional printed scaffolds

Bittner

Guo

2018

Bioprinting

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Three-dimensional printing (3DP) has enabled the fabrication of tissue engineering scaffolds that recapitulate the physical, architectural, and biochemical cues of native tissue matrix more effectively than ever before. One key component of biomimetic scaffold fabrication is the patterning of growth factors, whose spatial distribution and temporal release profile should ideally match that seen in native tissue development. Tissue engineers have made significant progress in improving the degree of spatiotemporal control over which growth factors are presented within 3DP scaffolds. However, significant limitations remain in terms in pattern resolution, the fabrication of true gradients, temporal control of growth factor release, the maintenance of growth factor distributions against diffusion, and more. This review summarizes several key areas for advancement of the field in terms of improving spatiotemporal control over growth factor presentation, and additionally highlights several major tissues of interest that have been targeted by 3DP growth factor patterning strategies.

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Electrical stimulation of adipose-derived mesenchymal stem cells in conductive scaffolds and the roles of voltage-gated ion channels

Cited by 156 publications

References 56 publications

Sequential application of mineralized electroconductive scaffold and electrical stimulation for efficient osteogenesis

Sequential application of mineralized electroconductive scaffold and electrical stimulation for efficient osteogenesis

Novel functions of the primary cilium in bone disease and cancer

Spatiotemporal control of growth factors in three-dimensional printed scaffolds

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